Metagenomic study of the communities of bacterial endophytes in the desert plant Senna Italica and their role in abiotic stress resistance in the plant
Abstract Plant leaves and roots are home to diverse communities of bacteria, which play a significant role in plant health and growth. Although one of the most unfriendly environments for plant growth is deserts, desert plants can influence their surrounding microbial population and choose favorable...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto Internacional de Ecologia
2022-11-01
|
| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100767&tlng=en |
| _version_ | 1811215424583368704 |
|---|---|
| author | Z. S. Alsaedi R. A. Ashy A. Y. Shami M. A. Majeed A. M. Alswat L. Baz M. N. Baeshen R. S. Jalal |
| author_facet | Z. S. Alsaedi R. A. Ashy A. Y. Shami M. A. Majeed A. M. Alswat L. Baz M. N. Baeshen R. S. Jalal |
| author_sort | Z. S. Alsaedi |
| collection | DOAJ |
| description | Abstract Plant leaves and roots are home to diverse communities of bacteria, which play a significant role in plant health and growth. Although one of the most unfriendly environments for plant growth is deserts, desert plants can influence their surrounding microbial population and choose favorable bacteria that encourage their growth under these severe circumstances. Senna italica is known for its excellent medicinal values as a traditional medical plant, but little is known about its associated endophytic bacterial community under extreme conditions. In the present study, metagenomic sequencing of 16S rRNA was used to report the diversity of endophytic bacterial communities associated with the leaves and roots of the desert medicinal plant Senna italica that was collected from the Asfan region in northeast Jeddah, Saudi Arabia. Analyses of the 16S rRNA sequences at the taxonomic phylum level revealed that bacterial communities in the roots and leaves samples belonged to five phyla, including Cyanobacteria, Proteobacteria, Actinobacteria, Firmicutes, and unclassified phyla. Results indicated that the most common phyla were Cyanobacteria/Chloroplast and Actinobacteria. Analysis of the 16S rRNA sequences at the taxonomic phylum level revealed that bacterial communities in the roots and leaves samples belonged to twelve genera at the taxonomic genus level. The most abundant ones were highlighted for further analysis, including Okibacterium and Streptomyces found in Actinobacteria, which were the dominant genus in roots samples. However, Streptophyta found in Cyanobacteria/Chloroplast was the dominant genus in leaf samples. Metagenomic analysis of medicinal plants leads to identifying novel organisms or genes that may have a role in abiotic stress resistance in the plant. The study of endophytic microbiome taxonomic, phylogenetic, and functional diversity will better know innovative candidates that may be selected as biological agents to enhance agricultural and industrial processes, especially for crop desert agricultural improvement. |
| first_indexed | 2024-04-12T06:21:11Z |
| format | Article |
| id | doaj.art-3c40b43fe9684fa3820ead28f89d240e |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-12T06:21:11Z |
| publishDate | 2022-11-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-3c40b43fe9684fa3820ead28f89d240e2022-12-22T03:44:17ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752022-11-018210.1590/1519-6984.267584Metagenomic study of the communities of bacterial endophytes in the desert plant Senna Italica and their role in abiotic stress resistance in the plantZ. S. Alsaedihttps://orcid.org/0000-0002-3398-6369R. A. Ashyhttps://orcid.org/0000-0003-3287-2332A. Y. Shamihttps://orcid.org/0000-0002-5336-038XM. A. Majeedhttps://orcid.org/0000-0001-6358-4144A. M. Alswathttps://orcid.org/0000-0002-8359-4558L. Bazhttps://orcid.org/0000-0003-4128-4196M. N. Baeshenhttps://orcid.org/0000-0002-0235-5222R. S. Jalalhttps://orcid.org/0000-0002-8372-9117Abstract Plant leaves and roots are home to diverse communities of bacteria, which play a significant role in plant health and growth. Although one of the most unfriendly environments for plant growth is deserts, desert plants can influence their surrounding microbial population and choose favorable bacteria that encourage their growth under these severe circumstances. Senna italica is known for its excellent medicinal values as a traditional medical plant, but little is known about its associated endophytic bacterial community under extreme conditions. In the present study, metagenomic sequencing of 16S rRNA was used to report the diversity of endophytic bacterial communities associated with the leaves and roots of the desert medicinal plant Senna italica that was collected from the Asfan region in northeast Jeddah, Saudi Arabia. Analyses of the 16S rRNA sequences at the taxonomic phylum level revealed that bacterial communities in the roots and leaves samples belonged to five phyla, including Cyanobacteria, Proteobacteria, Actinobacteria, Firmicutes, and unclassified phyla. Results indicated that the most common phyla were Cyanobacteria/Chloroplast and Actinobacteria. Analysis of the 16S rRNA sequences at the taxonomic phylum level revealed that bacterial communities in the roots and leaves samples belonged to twelve genera at the taxonomic genus level. The most abundant ones were highlighted for further analysis, including Okibacterium and Streptomyces found in Actinobacteria, which were the dominant genus in roots samples. However, Streptophyta found in Cyanobacteria/Chloroplast was the dominant genus in leaf samples. Metagenomic analysis of medicinal plants leads to identifying novel organisms or genes that may have a role in abiotic stress resistance in the plant. The study of endophytic microbiome taxonomic, phylogenetic, and functional diversity will better know innovative candidates that may be selected as biological agents to enhance agricultural and industrial processes, especially for crop desert agricultural improvement.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100767&tlng=enmicrobiomeendophytesPGPEBmetagenomicsSenna italica |
| spellingShingle | Z. S. Alsaedi R. A. Ashy A. Y. Shami M. A. Majeed A. M. Alswat L. Baz M. N. Baeshen R. S. Jalal Metagenomic study of the communities of bacterial endophytes in the desert plant Senna Italica and their role in abiotic stress resistance in the plant Brazilian Journal of Biology microbiome endophytes PGPEB metagenomics Senna italica |
| title | Metagenomic study of the communities of bacterial endophytes in the desert plant Senna Italica and their role in abiotic stress resistance in the plant |
| title_full | Metagenomic study of the communities of bacterial endophytes in the desert plant Senna Italica and their role in abiotic stress resistance in the plant |
| title_fullStr | Metagenomic study of the communities of bacterial endophytes in the desert plant Senna Italica and their role in abiotic stress resistance in the plant |
| title_full_unstemmed | Metagenomic study of the communities of bacterial endophytes in the desert plant Senna Italica and their role in abiotic stress resistance in the plant |
| title_short | Metagenomic study of the communities of bacterial endophytes in the desert plant Senna Italica and their role in abiotic stress resistance in the plant |
| title_sort | metagenomic study of the communities of bacterial endophytes in the desert plant senna italica and their role in abiotic stress resistance in the plant |
| topic | microbiome endophytes PGPEB metagenomics Senna italica |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100767&tlng=en |
| work_keys_str_mv | AT zsalsaedi metagenomicstudyofthecommunitiesofbacterialendophytesinthedesertplantsennaitalicaandtheirroleinabioticstressresistanceintheplant AT raashy metagenomicstudyofthecommunitiesofbacterialendophytesinthedesertplantsennaitalicaandtheirroleinabioticstressresistanceintheplant AT ayshami metagenomicstudyofthecommunitiesofbacterialendophytesinthedesertplantsennaitalicaandtheirroleinabioticstressresistanceintheplant AT mamajeed metagenomicstudyofthecommunitiesofbacterialendophytesinthedesertplantsennaitalicaandtheirroleinabioticstressresistanceintheplant AT amalswat metagenomicstudyofthecommunitiesofbacterialendophytesinthedesertplantsennaitalicaandtheirroleinabioticstressresistanceintheplant AT lbaz metagenomicstudyofthecommunitiesofbacterialendophytesinthedesertplantsennaitalicaandtheirroleinabioticstressresistanceintheplant AT mnbaeshen metagenomicstudyofthecommunitiesofbacterialendophytesinthedesertplantsennaitalicaandtheirroleinabioticstressresistanceintheplant AT rsjalal metagenomicstudyofthecommunitiesofbacterialendophytesinthedesertplantsennaitalicaandtheirroleinabioticstressresistanceintheplant |