Interleukin mRNA changes in mast cells stimulated by TSL-1 antigens
In this work we analyzed by RT-PCR, the mRNA changes for IL-4, IL-10, TNF and IFN ( induced by TSL-1 antigens in a rat mast cell line (HRMC) with mucosal characteristics. The data obtained showed an increase of 65 and 52 % in mRNA expression for IL-4 and TNF respectively and a decrease of 59 and 55...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2001-06-01
|
| Series: | Parasite |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1051/parasite/200108s2114 |
| _version_ | 1797427412421574656 |
|---|---|
| author | Arizmendi N. Yépez-Mulia L. Cedillo-Rivera R. Ortega-Pierres M.G. Muñoz O. Befus D. Enciso-Moreno J.A |
| author_facet | Arizmendi N. Yépez-Mulia L. Cedillo-Rivera R. Ortega-Pierres M.G. Muñoz O. Befus D. Enciso-Moreno J.A |
| author_sort | Arizmendi N. |
| collection | DOAJ |
| description | In this work we analyzed by RT-PCR, the mRNA changes for IL-4, IL-10, TNF and IFN ( induced by TSL-1 antigens in a rat mast cell line (HRMC) with mucosal characteristics. The data obtained showed an increase of 65 and 52 % in mRNA expression for IL-4 and TNF respectively and a decrease of 59 and 55 % in mRNAs for IFNγ and IL-10. Our results suggest that TSL-1 antigens induce the release from MC of regulatory molecules, such as IL-4 by an IgE independent mechanism. Our data also provides important information related to the ability of MC to participate not only in the effector phase against the infectious agents, but also in the orchestration of the immune response by the host against parasites. |
| first_indexed | 2024-03-09T08:43:45Z |
| format | Article |
| id | doaj.art-3c40b5d259d74185bb516849b36d75d8 |
| institution | Directory Open Access Journal |
| issn | 1252-607X 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T08:43:45Z |
| publishDate | 2001-06-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-3c40b5d259d74185bb516849b36d75d82023-12-02T15:51:54ZengEDP SciencesParasite1252-607X1776-10422001-06-018S114S11610.1051/parasite/200108s2114parasite200108s2p114Interleukin mRNA changes in mast cells stimulated by TSL-1 antigensArizmendi N.Yépez-Mulia L.Cedillo-Rivera R.Ortega-Pierres M.G.Muñoz O.Befus D.Enciso-Moreno J.AIn this work we analyzed by RT-PCR, the mRNA changes for IL-4, IL-10, TNF and IFN ( induced by TSL-1 antigens in a rat mast cell line (HRMC) with mucosal characteristics. The data obtained showed an increase of 65 and 52 % in mRNA expression for IL-4 and TNF respectively and a decrease of 59 and 55 % in mRNAs for IFNγ and IL-10. Our results suggest that TSL-1 antigens induce the release from MC of regulatory molecules, such as IL-4 by an IgE independent mechanism. Our data also provides important information related to the ability of MC to participate not only in the effector phase against the infectious agents, but also in the orchestration of the immune response by the host against parasites.http://dx.doi.org/10.1051/parasite/200108s2114mast cellactivationTSL-1 antigensRT-PCRTrichinella spiralis |
| spellingShingle | Arizmendi N. Yépez-Mulia L. Cedillo-Rivera R. Ortega-Pierres M.G. Muñoz O. Befus D. Enciso-Moreno J.A Interleukin mRNA changes in mast cells stimulated by TSL-1 antigens Parasite mast cell activation TSL-1 antigens RT-PCR Trichinella spiralis |
| title | Interleukin mRNA changes in mast cells stimulated by TSL-1 antigens |
| title_full | Interleukin mRNA changes in mast cells stimulated by TSL-1 antigens |
| title_fullStr | Interleukin mRNA changes in mast cells stimulated by TSL-1 antigens |
| title_full_unstemmed | Interleukin mRNA changes in mast cells stimulated by TSL-1 antigens |
| title_short | Interleukin mRNA changes in mast cells stimulated by TSL-1 antigens |
| title_sort | interleukin mrna changes in mast cells stimulated by tsl 1 antigens |
| topic | mast cell activation TSL-1 antigens RT-PCR Trichinella spiralis |
| url | http://dx.doi.org/10.1051/parasite/200108s2114 |
| work_keys_str_mv | AT arizmendin interleukinmrnachangesinmastcellsstimulatedbytsl1antigens AT yepezmulial interleukinmrnachangesinmastcellsstimulatedbytsl1antigens AT cedilloriverar interleukinmrnachangesinmastcellsstimulatedbytsl1antigens AT ortegapierresmg interleukinmrnachangesinmastcellsstimulatedbytsl1antigens AT munozo interleukinmrnachangesinmastcellsstimulatedbytsl1antigens AT befusd interleukinmrnachangesinmastcellsstimulatedbytsl1antigens AT encisomorenoja interleukinmrnachangesinmastcellsstimulatedbytsl1antigens |