Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells
<i>Trigonella foenum-graecum</i> L. (fenugreek), a member of the legume family (Fabaceae), is a promising source of bioactive phytochemicals, which explains its traditional use for a variety of metabolic disorders including cancer. The current study aimed to evaluate extracts of fenugree...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/14/4/784 |
| _version_ | 1797477441564835840 |
|---|---|
| author | Kholoud K. Khoja Melanie-Jayne R. Howes Robert Hider Paul A. Sharp Iain W. Farrell Gladys O. Latunde-Dada |
| author_facet | Kholoud K. Khoja Melanie-Jayne R. Howes Robert Hider Paul A. Sharp Iain W. Farrell Gladys O. Latunde-Dada |
| author_sort | Kholoud K. Khoja |
| collection | DOAJ |
| description | <i>Trigonella foenum-graecum</i> L. (fenugreek), a member of the legume family (Fabaceae), is a promising source of bioactive phytochemicals, which explains its traditional use for a variety of metabolic disorders including cancer. The current study aimed to evaluate extracts of fenugreek seeds and sprouts, and some of their constituents, to compare their cytotoxic and antiproliferative activities in MCF-7 breast cancer cells. The extracts were chemically characterised using high-resolution accurate mass liquid chromatography-mass spectrometry to reveal the detection of compounds assigned as flavone <i>C</i>-glycosides including those derived from apigenin and luteolin, in addition to isoflavones. Five different flavones or their glycosides (apigenin, vicenin-2, vitexin, luteolin and orientin) and two isoflavones (daidzein and formononetin) were quantified in the fenugreek extracts. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay using MCF-7 cells treated with fenugreek methanolic extracts showed dose- and time-dependent effects on cell viability. The MCF-7 cancer cells treated with the fenugreek methanolic extracts also displayed increased relative mitochondrial DNA damage as well as suppressed metastasis and proliferation. This study demonstrates the potential anti-cancer effects of fenugreek seeds and sprouts and reveals fenugreek sprouts as an untapped resource for bioactive compounds. |
| first_indexed | 2024-03-09T21:17:42Z |
| format | Article |
| id | doaj.art-3c41c4283d42492ea10c1cd841b1adb2 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T21:17:42Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-3c41c4283d42492ea10c1cd841b1adb22023-11-23T21:28:20ZengMDPI AGNutrients2072-66432022-02-0114478410.3390/nu14040784Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer CellsKholoud K. Khoja0Melanie-Jayne R. Howes1Robert Hider2Paul A. Sharp3Iain W. Farrell4Gladys O. Latunde-Dada5Department of Nutritional Sciences, School of Life Course Sciences, King’s College London, Franklin-Wilkins-Building, 150 Stamford Street, London SE1 9NH, UKRoyal Botanic Gardens Kew, Richmond TW9 3DS, UKInstitute of Pharmaceutical Science, Faculty of Life Sciences and Medicine, King’s College London, Franklin-Wilkins-Building, 150 Stamford Street, London SE1 9NH, UKDepartment of Nutritional Sciences, School of Life Course Sciences, King’s College London, Franklin-Wilkins-Building, 150 Stamford Street, London SE1 9NH, UKRoyal Botanic Gardens Kew, Richmond TW9 3DS, UKDepartment of Nutritional Sciences, School of Life Course Sciences, King’s College London, Franklin-Wilkins-Building, 150 Stamford Street, London SE1 9NH, UK<i>Trigonella foenum-graecum</i> L. (fenugreek), a member of the legume family (Fabaceae), is a promising source of bioactive phytochemicals, which explains its traditional use for a variety of metabolic disorders including cancer. The current study aimed to evaluate extracts of fenugreek seeds and sprouts, and some of their constituents, to compare their cytotoxic and antiproliferative activities in MCF-7 breast cancer cells. The extracts were chemically characterised using high-resolution accurate mass liquid chromatography-mass spectrometry to reveal the detection of compounds assigned as flavone <i>C</i>-glycosides including those derived from apigenin and luteolin, in addition to isoflavones. Five different flavones or their glycosides (apigenin, vicenin-2, vitexin, luteolin and orientin) and two isoflavones (daidzein and formononetin) were quantified in the fenugreek extracts. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay using MCF-7 cells treated with fenugreek methanolic extracts showed dose- and time-dependent effects on cell viability. The MCF-7 cancer cells treated with the fenugreek methanolic extracts also displayed increased relative mitochondrial DNA damage as well as suppressed metastasis and proliferation. This study demonstrates the potential anti-cancer effects of fenugreek seeds and sprouts and reveals fenugreek sprouts as an untapped resource for bioactive compounds.https://www.mdpi.com/2072-6643/14/4/784fenugreekcytotoxicityMCF-7ironcancer |
| spellingShingle | Kholoud K. Khoja Melanie-Jayne R. Howes Robert Hider Paul A. Sharp Iain W. Farrell Gladys O. Latunde-Dada Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells Nutrients fenugreek cytotoxicity MCF-7 iron cancer |
| title | Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells |
| title_full | Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells |
| title_fullStr | Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells |
| title_full_unstemmed | Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells |
| title_short | Cytotoxicity of Fenugreek Sprout and Seed Extracts and Their Bioactive Constituents on MCF-7 Breast Cancer Cells |
| title_sort | cytotoxicity of fenugreek sprout and seed extracts and their bioactive constituents on mcf 7 breast cancer cells |
| topic | fenugreek cytotoxicity MCF-7 iron cancer |
| url | https://www.mdpi.com/2072-6643/14/4/784 |
| work_keys_str_mv | AT kholoudkkhoja cytotoxicityoffenugreeksproutandseedextractsandtheirbioactiveconstituentsonmcf7breastcancercells AT melaniejaynerhowes cytotoxicityoffenugreeksproutandseedextractsandtheirbioactiveconstituentsonmcf7breastcancercells AT roberthider cytotoxicityoffenugreeksproutandseedextractsandtheirbioactiveconstituentsonmcf7breastcancercells AT paulasharp cytotoxicityoffenugreeksproutandseedextractsandtheirbioactiveconstituentsonmcf7breastcancercells AT iainwfarrell cytotoxicityoffenugreeksproutandseedextractsandtheirbioactiveconstituentsonmcf7breastcancercells AT gladysolatundedada cytotoxicityoffenugreeksproutandseedextractsandtheirbioactiveconstituentsonmcf7breastcancercells |