DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment
Pandemics require a fast and immediate response to contain potential infectious carriers. In the recent 2020 Covid-19 worldwide pandemic, authorities all around the world have failed to identify potential carriers and contain it on time. Hence, a rapid and very sensitive testing method is required....
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/16/4648 |
| _version_ | 1797557288802713600 |
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| author | Manikandan Santhanam Itay Algov Lital Alfonta |
| author_facet | Manikandan Santhanam Itay Algov Lital Alfonta |
| author_sort | Manikandan Santhanam |
| collection | DOAJ |
| description | Pandemics require a fast and immediate response to contain potential infectious carriers. In the recent 2020 Covid-19 worldwide pandemic, authorities all around the world have failed to identify potential carriers and contain it on time. Hence, a rapid and very sensitive testing method is required. Current diagnostic tools, reverse transcription PCR (RT-PCR) and real-time PCR (qPCR), have its pitfalls for quick pandemic containment such as the requirement for specialized professionals and instrumentation. Versatile electrochemical DNA/RNA sensors are a promising technological alternative for PCR based diagnosis. In an electrochemical DNA sensor, a nucleic acid hybridization event is converted into a quantifiable electrochemical signal. A critical challenge of electrochemical DNA sensors is sensitive detection of a low copy number of DNA/RNA in samples such as is the case for early onset of a disease. Signal amplification approaches are an important tool to overcome this sensitivity issue. In this review, the authors discuss the most recent signal amplification strategies employed in the electrochemical DNA/RNA diagnosis of pathogens. |
| first_indexed | 2024-03-10T17:15:01Z |
| format | Article |
| id | doaj.art-3c427c8d98f7455db08160ba945991ef |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T17:15:01Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-3c427c8d98f7455db08160ba945991ef2023-11-20T10:31:33ZengMDPI AGSensors1424-82202020-08-012016464810.3390/s20164648DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic ContainmentManikandan Santhanam0Itay Algov1Lital Alfonta2Departments of Life Sciences, Chemistry and Ilse Katz Institute for Nanoscale Science and Technology, PO Box 653, Ben-Gurion University of the Negev, Beer-Sheva 8410501, IsraelDepartments of Life Sciences, Chemistry and Ilse Katz Institute for Nanoscale Science and Technology, PO Box 653, Ben-Gurion University of the Negev, Beer-Sheva 8410501, IsraelDepartments of Life Sciences, Chemistry and Ilse Katz Institute for Nanoscale Science and Technology, PO Box 653, Ben-Gurion University of the Negev, Beer-Sheva 8410501, IsraelPandemics require a fast and immediate response to contain potential infectious carriers. In the recent 2020 Covid-19 worldwide pandemic, authorities all around the world have failed to identify potential carriers and contain it on time. Hence, a rapid and very sensitive testing method is required. Current diagnostic tools, reverse transcription PCR (RT-PCR) and real-time PCR (qPCR), have its pitfalls for quick pandemic containment such as the requirement for specialized professionals and instrumentation. Versatile electrochemical DNA/RNA sensors are a promising technological alternative for PCR based diagnosis. In an electrochemical DNA sensor, a nucleic acid hybridization event is converted into a quantifiable electrochemical signal. A critical challenge of electrochemical DNA sensors is sensitive detection of a low copy number of DNA/RNA in samples such as is the case for early onset of a disease. Signal amplification approaches are an important tool to overcome this sensitivity issue. In this review, the authors discuss the most recent signal amplification strategies employed in the electrochemical DNA/RNA diagnosis of pathogens.https://www.mdpi.com/1424-8220/20/16/4648electrochemical DNA sensornucleic acid sensorsignal amplificationDNARNApathogen sensing |
| spellingShingle | Manikandan Santhanam Itay Algov Lital Alfonta DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment Sensors electrochemical DNA sensor nucleic acid sensor signal amplification DNA RNA pathogen sensing |
| title | DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment |
| title_full | DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment |
| title_fullStr | DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment |
| title_full_unstemmed | DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment |
| title_short | DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment |
| title_sort | dna rna electrochemical biosensing devices a future replacement of pcr methods for a fast epidemic containment |
| topic | electrochemical DNA sensor nucleic acid sensor signal amplification DNA RNA pathogen sensing |
| url | https://www.mdpi.com/1424-8220/20/16/4648 |
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