An Evaluation of the Efficacy and Safety of TAMIXAM<sup>®</sup>, Based on Hyaluronic Acid and Tamarind Seed Extract, for Esophageal Mucosal Protection from Acid Insult
TAMIXAM<sup>®</sup> is a novel technology that combines hyaluronic acid and tamarind seed extract in its formulation. It is designed to protect the esophageal mucosa by creating a barrier through its filmogenic properties. The aim of this study is to evaluate the safety and efficacy of t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Gastrointestinal Disorders |
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| Online Access: | https://www.mdpi.com/2624-5647/6/1/15 |
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| author | Marisa Francesca Motta Giuseppe Pulitano Antonino Bagnulo Giampaolo Buriani Umberto Di Maio Fabio Amone Vincenzo Nobile Rocco Malivindi |
| author_facet | Marisa Francesca Motta Giuseppe Pulitano Antonino Bagnulo Giampaolo Buriani Umberto Di Maio Fabio Amone Vincenzo Nobile Rocco Malivindi |
| author_sort | Marisa Francesca Motta |
| collection | DOAJ |
| description | TAMIXAM<sup>®</sup> is a novel technology that combines hyaluronic acid and tamarind seed extract in its formulation. It is designed to protect the esophageal mucosa by creating a barrier through its filmogenic properties. The aim of this study is to evaluate the safety and efficacy of this technology through mucoadhesion tests, a cell viability assay, TEER measurements, and morphological analysis on reconstructed esophageal mucosa exposed to 10% hydrochloric acid before and after treatment. The mucoadhesion test highlighted the synergistic bioadhesive effect of the technology’s components. Cell viability assays revealed the substantial mucoprotective and barrier effects of the technology, preserving tissue viability when applied before exposure to acid insult. A morphological analysis illustrated TAMIXAM<sup>®</sup>’s efficacy in countering acid-induced damage, reducing erosion, necrosis, and tissue degeneration compared to the positive control, both pre- and post-acid insult. An evaluation of epithelial integrity through TEER measurements indicated a minimal reduction in tissues treated with the invention before acid exposure, demonstrating its ability to maintain epithelial integrity in the presence of an acid insult. However, this effect was less pronounced in tissues treated with the technology after the acid insult, implying a potential partial recovery of epithelial integrity. Furthermore, comprehensive in vitro and in vivo studies supported the safety profile of the invention. In conclusion, TAMIXAM<sup>®</sup> emerged as a compelling solution, providing enhanced mechanical action to maintain epithelial balance and shield the esophageal mucosa from acid-induced damage. |
| first_indexed | 2024-04-24T18:14:26Z |
| format | Article |
| id | doaj.art-3c4342115ec64f9bb980d7935ec19977 |
| institution | Directory Open Access Journal |
| issn | 2624-5647 |
| language | English |
| last_indexed | 2024-04-24T18:14:26Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gastrointestinal Disorders |
| spelling | doaj.art-3c4342115ec64f9bb980d7935ec199772024-03-27T13:42:32ZengMDPI AGGastrointestinal Disorders2624-56472024-02-016120221310.3390/gidisord6010015An Evaluation of the Efficacy and Safety of TAMIXAM<sup>®</sup>, Based on Hyaluronic Acid and Tamarind Seed Extract, for Esophageal Mucosal Protection from Acid InsultMarisa Francesca Motta0Giuseppe Pulitano1Antonino Bagnulo2Giampaolo Buriani3Umberto Di Maio4Fabio Amone5Vincenzo Nobile6Rocco Malivindi7Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, CS, ItalyNutratech S.r.l., c/o Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, CS, ItalyNeilos S.r.l., Via Bagnulo 95, 80063 Piano di Sorrento, NA, ItalyBiochem S.r.l., Via G.Benini 13, 40069 Zola Predosa, BO, ItalyShedir Pharma Group S.p.A., Via Bagnulo 95, 80063 Piano di Sorrento, NA, ItalyNutratech S.r.l., c/o Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, CS, ItalyComplife Italia S.r.l., 27028 San Martino Siccomario, PV, ItalyDepartment of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende, CS, ItalyTAMIXAM<sup>®</sup> is a novel technology that combines hyaluronic acid and tamarind seed extract in its formulation. It is designed to protect the esophageal mucosa by creating a barrier through its filmogenic properties. The aim of this study is to evaluate the safety and efficacy of this technology through mucoadhesion tests, a cell viability assay, TEER measurements, and morphological analysis on reconstructed esophageal mucosa exposed to 10% hydrochloric acid before and after treatment. The mucoadhesion test highlighted the synergistic bioadhesive effect of the technology’s components. Cell viability assays revealed the substantial mucoprotective and barrier effects of the technology, preserving tissue viability when applied before exposure to acid insult. A morphological analysis illustrated TAMIXAM<sup>®</sup>’s efficacy in countering acid-induced damage, reducing erosion, necrosis, and tissue degeneration compared to the positive control, both pre- and post-acid insult. An evaluation of epithelial integrity through TEER measurements indicated a minimal reduction in tissues treated with the invention before acid exposure, demonstrating its ability to maintain epithelial integrity in the presence of an acid insult. However, this effect was less pronounced in tissues treated with the technology after the acid insult, implying a potential partial recovery of epithelial integrity. Furthermore, comprehensive in vitro and in vivo studies supported the safety profile of the invention. In conclusion, TAMIXAM<sup>®</sup> emerged as a compelling solution, providing enhanced mechanical action to maintain epithelial balance and shield the esophageal mucosa from acid-induced damage.https://www.mdpi.com/2624-5647/6/1/15gastroesophageal reflux diseasehyaluronic acidbarrier effect |
| spellingShingle | Marisa Francesca Motta Giuseppe Pulitano Antonino Bagnulo Giampaolo Buriani Umberto Di Maio Fabio Amone Vincenzo Nobile Rocco Malivindi An Evaluation of the Efficacy and Safety of TAMIXAM<sup>®</sup>, Based on Hyaluronic Acid and Tamarind Seed Extract, for Esophageal Mucosal Protection from Acid Insult Gastrointestinal Disorders gastroesophageal reflux disease hyaluronic acid barrier effect |
| title | An Evaluation of the Efficacy and Safety of TAMIXAM<sup>®</sup>, Based on Hyaluronic Acid and Tamarind Seed Extract, for Esophageal Mucosal Protection from Acid Insult |
| title_full | An Evaluation of the Efficacy and Safety of TAMIXAM<sup>®</sup>, Based on Hyaluronic Acid and Tamarind Seed Extract, for Esophageal Mucosal Protection from Acid Insult |
| title_fullStr | An Evaluation of the Efficacy and Safety of TAMIXAM<sup>®</sup>, Based on Hyaluronic Acid and Tamarind Seed Extract, for Esophageal Mucosal Protection from Acid Insult |
| title_full_unstemmed | An Evaluation of the Efficacy and Safety of TAMIXAM<sup>®</sup>, Based on Hyaluronic Acid and Tamarind Seed Extract, for Esophageal Mucosal Protection from Acid Insult |
| title_short | An Evaluation of the Efficacy and Safety of TAMIXAM<sup>®</sup>, Based on Hyaluronic Acid and Tamarind Seed Extract, for Esophageal Mucosal Protection from Acid Insult |
| title_sort | evaluation of the efficacy and safety of tamixam sup r sup based on hyaluronic acid and tamarind seed extract for esophageal mucosal protection from acid insult |
| topic | gastroesophageal reflux disease hyaluronic acid barrier effect |
| url | https://www.mdpi.com/2624-5647/6/1/15 |
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