Location and functions of Inebriated in the Drosophila eye
Histamine (HA) is a neurotransmitter in arthropod photoreceptors. It is recycled via conjugation to β-alanine to form β-alanylhistamine (carcinine). Conjugation occurs in epithelial glia that surround photoreceptor terminals in the first optic neuropil, and carcinine (CA) is then transported back to...
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| Format: | Article |
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The Company of Biologists
2018-07-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/7/7/bio034926 |
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| author | Janusz Borycz Anna Ziegler Jolanta A. Borycz Guido Uhlenbrock Daniel Tapken Lucia Caceres Michael Hollmann Bernhard T. Hovemann Ian A. Meinertzhagen |
| author_facet | Janusz Borycz Anna Ziegler Jolanta A. Borycz Guido Uhlenbrock Daniel Tapken Lucia Caceres Michael Hollmann Bernhard T. Hovemann Ian A. Meinertzhagen |
| author_sort | Janusz Borycz |
| collection | DOAJ |
| description | Histamine (HA) is a neurotransmitter in arthropod photoreceptors. It is recycled via conjugation to β-alanine to form β-alanylhistamine (carcinine). Conjugation occurs in epithelial glia that surround photoreceptor terminals in the first optic neuropil, and carcinine (CA) is then transported back to photoreceptors and cleaved to liberate HA and β-alanine. The gene Inebriated (Ine) encodes an Na+/Cl−-dependent SLC6 family transporter translated as two protein isoforms, long (P1) and short (P2). Photoreceptors specifically express Ine-P2 whereas Ine-P1 is expressed in non-neuronal cells. Both ine1 and ine3 have significantly reduced head HA contents compared with wild type, and a smaller increase in head HA after drinking 1% CA. Similarly, uptake of 0.1% CA was reduced in ine1 and ine3 mutant synaptosomes, but increased by 90% and 84% respectively for fractions incubated in 0.05% β-Ala, compared with wild type. Screening potential substrates in Ine expressing Xenopus oocytes revealed very little response to carcinine and β-Ala but increased conductance with glycine. Both ine1 and ine3 mutant responses in light-dark phototaxis did not differ from wild-type. Collectively our results suggest that Inebriated functions in an adjunct role as a transporter to the previously reported carcinine transporter CarT. |
| first_indexed | 2024-12-18T00:03:47Z |
| format | Article |
| id | doaj.art-3c4513eac93f43e9974bd1521ea27238 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-18T00:03:47Z |
| publishDate | 2018-07-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-3c4513eac93f43e9974bd1521ea272382022-12-21T21:27:53ZengThe Company of BiologistsBiology Open2046-63902018-07-017710.1242/bio.034926034926Location and functions of Inebriated in the Drosophila eyeJanusz Borycz0Anna Ziegler1Jolanta A. Borycz2Guido Uhlenbrock3Daniel Tapken4Lucia Caceres5Michael Hollmann6Bernhard T. Hovemann7Ian A. Meinertzhagen8 Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada Receptor Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada Receptor Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany Receptor Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada Molecular Cell Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany Receptor Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University of Bochum, 44780 Bochum, Germany Department of Psychology and Neuroscience, Dalhousie University, Halifax, NS B3H 4R2, Canada Histamine (HA) is a neurotransmitter in arthropod photoreceptors. It is recycled via conjugation to β-alanine to form β-alanylhistamine (carcinine). Conjugation occurs in epithelial glia that surround photoreceptor terminals in the first optic neuropil, and carcinine (CA) is then transported back to photoreceptors and cleaved to liberate HA and β-alanine. The gene Inebriated (Ine) encodes an Na+/Cl−-dependent SLC6 family transporter translated as two protein isoforms, long (P1) and short (P2). Photoreceptors specifically express Ine-P2 whereas Ine-P1 is expressed in non-neuronal cells. Both ine1 and ine3 have significantly reduced head HA contents compared with wild type, and a smaller increase in head HA after drinking 1% CA. Similarly, uptake of 0.1% CA was reduced in ine1 and ine3 mutant synaptosomes, but increased by 90% and 84% respectively for fractions incubated in 0.05% β-Ala, compared with wild type. Screening potential substrates in Ine expressing Xenopus oocytes revealed very little response to carcinine and β-Ala but increased conductance with glycine. Both ine1 and ine3 mutant responses in light-dark phototaxis did not differ from wild-type. Collectively our results suggest that Inebriated functions in an adjunct role as a transporter to the previously reported carcinine transporter CarT.http://bio.biologists.org/content/7/7/bio034926DrosophilaHistamineCarcinineGlycineTransportElectroretinogramPhototaxisImmunocytochemistry |
| spellingShingle | Janusz Borycz Anna Ziegler Jolanta A. Borycz Guido Uhlenbrock Daniel Tapken Lucia Caceres Michael Hollmann Bernhard T. Hovemann Ian A. Meinertzhagen Location and functions of Inebriated in the Drosophila eye Biology Open Drosophila Histamine Carcinine Glycine Transport Electroretinogram Phototaxis Immunocytochemistry |
| title | Location and functions of Inebriated in the Drosophila eye |
| title_full | Location and functions of Inebriated in the Drosophila eye |
| title_fullStr | Location and functions of Inebriated in the Drosophila eye |
| title_full_unstemmed | Location and functions of Inebriated in the Drosophila eye |
| title_short | Location and functions of Inebriated in the Drosophila eye |
| title_sort | location and functions of inebriated in the drosophila eye |
| topic | Drosophila Histamine Carcinine Glycine Transport Electroretinogram Phototaxis Immunocytochemistry |
| url | http://bio.biologists.org/content/7/7/bio034926 |
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