FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE
Aim. To characterize pool of Crohn’s disease-associated E.coli isolated from patients with Crohn’s disease. Materials and methods. 28 clinical stains were selected. Clinical isolates, as well as control samples, were tested for their possible belonging to a certain phylogenetic group, the ability to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2017-12-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/229 |
| _version_ | 1828435007664816128 |
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| author | R. B. Gorodnichev D. V. Rakitina A. I. Manolov Yu. P. Baykova P. L. Scherbakov G. B. Smirnov E. N. Ilina |
| author_facet | R. B. Gorodnichev D. V. Rakitina A. I. Manolov Yu. P. Baykova P. L. Scherbakov G. B. Smirnov E. N. Ilina |
| author_sort | R. B. Gorodnichev |
| collection | DOAJ |
| description | Aim. To characterize pool of Crohn’s disease-associated E.coli isolated from patients with Crohn’s disease. Materials and methods. 28 clinical stains were selected. Clinical isolates, as well as control samples, were tested for their possible belonging to a certain phylogenetic group, the ability to adhere and invade on the model of a monolayer of CaCo2 epithelial cells, the ability to form biofilms and their mobility. Results. We have shown that E.coli, isolated from a different biomaterial, belonged to different phylogenetic groups and differed in their ability to form biofilms. Adhesive-invasive E.coli were found mainly in the material of biopsy of the intestinal mucosa of patients with Crohn’s disease. Conclusion. There are reasons to suppose that adhesive-invasive E.coli formed at the junction of ecological niches of the lumen and the intestinal wall. |
| first_indexed | 2024-12-10T19:00:19Z |
| format | Article |
| id | doaj.art-3c45dd4dfee04a4cbf1afceb5eadebb5 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-12-10T19:00:19Z |
| publishDate | 2017-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-3c45dd4dfee04a4cbf1afceb5eadebb52022-12-22T01:37:02ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132017-12-0106424910.36233/0372-9311-2017-6-42-49229FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASER. B. Gorodnichev0D. V. Rakitina1A. I. Manolov2Yu. P. Baykova3P. L. Scherbakov4G. B. Smirnov5E. N. Ilina6Federal Research and Clinical Center of Physical-Chemical MedicineFederal Research and Clinical Center of Physical-Chemical MedicineFederal Research and Clinical Center of Physical-Chemical MedicineFederal Research and Clinical Center of Physical-Chemical MedicineFederal Research and Clinical Center of Physical-Chemical MedicineFederal Research and Clinical Center of Physical-Chemical MedicineFederal Research and Clinical Center of Physical-Chemical MedicineAim. To characterize pool of Crohn’s disease-associated E.coli isolated from patients with Crohn’s disease. Materials and methods. 28 clinical stains were selected. Clinical isolates, as well as control samples, were tested for their possible belonging to a certain phylogenetic group, the ability to adhere and invade on the model of a monolayer of CaCo2 epithelial cells, the ability to form biofilms and their mobility. Results. We have shown that E.coli, isolated from a different biomaterial, belonged to different phylogenetic groups and differed in their ability to form biofilms. Adhesive-invasive E.coli were found mainly in the material of biopsy of the intestinal mucosa of patients with Crohn’s disease. Conclusion. There are reasons to suppose that adhesive-invasive E.coli formed at the junction of ecological niches of the lumen and the intestinal wall.https://microbiol.elpub.ru/jour/article/view/229crohn’s diseaseadhesive-invasive e.coliheterological modelsbiofilmsphylogenetic diversity of e.coli |
| spellingShingle | R. B. Gorodnichev D. V. Rakitina A. I. Manolov Yu. P. Baykova P. L. Scherbakov G. B. Smirnov E. N. Ilina FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE Журнал микробиологии, эпидемиологии и иммунобиологии crohn’s disease adhesive-invasive e.coli heterological models biofilms phylogenetic diversity of e.coli |
| title | FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE |
| title_full | FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE |
| title_fullStr | FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE |
| title_full_unstemmed | FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE |
| title_short | FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE |
| title_sort | features of escherichia coli clinical strains isolated from the patients with crohn s disease |
| topic | crohn’s disease adhesive-invasive e.coli heterological models biofilms phylogenetic diversity of e.coli |
| url | https://microbiol.elpub.ru/jour/article/view/229 |
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