Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip
Hepatitis B virus infection is not only a huge burden in the field of social health but also a major public health problem that affects the lives and health of the people. Simple, rapid, feasible detection of HBV is critical for its prevention and spread, especially in the developing countries with...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.763079/full |
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| author | Mao-Ling Sun Hai-Yun Lai Na-Yu Chong Dong-Fan Liu Zhen-Yi Zhang Bo Pang Jun Yao |
| author_facet | Mao-Ling Sun Hai-Yun Lai Na-Yu Chong Dong-Fan Liu Zhen-Yi Zhang Bo Pang Jun Yao |
| author_sort | Mao-Ling Sun |
| collection | DOAJ |
| description | Hepatitis B virus infection is not only a huge burden in the field of social health but also a major public health problem that affects the lives and health of the people. Simple, rapid, feasible detection of HBV is critical for its prevention and spread, especially in the developing countries with low-resource laboratories. To this end, we combined multienzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) strip to detect HBV. A pair of primers targeting the conserved region of HBV genome was designed and used in MIRA-LFD assay. Our results found that the entire amplification of MIRA-LFD only takes 10 min at 37°C and the dilution of the amplification products was added in the LFD strip and observed by the naked eye after 10 min. The detection sensitivity of this method can reach 10 pg. The 45 clinical samples were detected by MIRA-LFD and real-time PCR. The accuracy rate of MIRA-LFD was 100%. Therefore, these characteristics of our newly developed MIRA-LFD assay make it particularly useful and suitable for detecting HBV in the resource-limited condition. |
| first_indexed | 2024-12-21T02:20:28Z |
| format | Article |
| id | doaj.art-3c4947f6c42a4e6bbc3fd0ca3ebc7934 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-21T02:20:28Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-3c4947f6c42a4e6bbc3fd0ca3ebc79342022-12-21T19:19:10ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-12-01810.3389/fmolb.2021.763079763079Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick StripMao-Ling SunHai-Yun LaiNa-Yu ChongDong-Fan LiuZhen-Yi ZhangBo PangJun YaoHepatitis B virus infection is not only a huge burden in the field of social health but also a major public health problem that affects the lives and health of the people. Simple, rapid, feasible detection of HBV is critical for its prevention and spread, especially in the developing countries with low-resource laboratories. To this end, we combined multienzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) strip to detect HBV. A pair of primers targeting the conserved region of HBV genome was designed and used in MIRA-LFD assay. Our results found that the entire amplification of MIRA-LFD only takes 10 min at 37°C and the dilution of the amplification products was added in the LFD strip and observed by the naked eye after 10 min. The detection sensitivity of this method can reach 10 pg. The 45 clinical samples were detected by MIRA-LFD and real-time PCR. The accuracy rate of MIRA-LFD was 100%. Therefore, these characteristics of our newly developed MIRA-LFD assay make it particularly useful and suitable for detecting HBV in the resource-limited condition.https://www.frontiersin.org/articles/10.3389/fmolb.2021.763079/fullhepatitis B virusmultienzyme isothermal rapid amplificationlateral flow stripdetectionnucleic acid |
| spellingShingle | Mao-Ling Sun Hai-Yun Lai Na-Yu Chong Dong-Fan Liu Zhen-Yi Zhang Bo Pang Jun Yao Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip Frontiers in Molecular Biosciences hepatitis B virus multienzyme isothermal rapid amplification lateral flow strip detection nucleic acid |
| title | Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip |
| title_full | Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip |
| title_fullStr | Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip |
| title_full_unstemmed | Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip |
| title_short | Simple and Feasible Detection of Hepatitis B Virus via Combination of Multienzyme Isothermal Rapid Amplification and Lateral Flow Dipstick Strip |
| title_sort | simple and feasible detection of hepatitis b virus via combination of multienzyme isothermal rapid amplification and lateral flow dipstick strip |
| topic | hepatitis B virus multienzyme isothermal rapid amplification lateral flow strip detection nucleic acid |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.763079/full |
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