Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls
This is a broad overview and critical review of a particular group of closely related ex vivo and in vitro metabolic NMR spectroscopic methods. The scope of interest comprises studies of cultured cells and excised tissue, either intact or after physicochemical extraction of metabolites. Our detailed...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/13/4214 |
| _version_ | 1797408562915311616 |
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| author | Norbert W. Lutz Monique Bernard |
| author_facet | Norbert W. Lutz Monique Bernard |
| author_sort | Norbert W. Lutz |
| collection | DOAJ |
| description | This is a broad overview and critical review of a particular group of closely related ex vivo and in vitro metabolic NMR spectroscopic methods. The scope of interest comprises studies of cultured cells and excised tissue, either intact or after physicochemical extraction of metabolites. Our detailed discussion includes pitfalls that have led to erroneous statements in the published literature, some of which may cause serious problems in metabolic and biological interpretation of results. To cover a wide range of work from relevant research areas, we consider not only the most recent achievements in the field, but also techniques that proved to be valid and successful in the past, although they may not have generated a very significant number of papers more recently. Thus, this comparative review also aims at providing background information useful for judiciously choosing between the metabolic ex vivo/in vitro NMR methods presented. Finally, the methods of interest are discussed in the context of, and in relation to, other metabolic analysis protocols such as HR-MAS and cell perfusion NMR, as well as the mass spectrometry approach. |
| first_indexed | 2024-03-09T04:00:15Z |
| format | Article |
| id | doaj.art-3c4ad608cbd849b9ad827d53a8ab38cf |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:00:15Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3c4ad608cbd849b9ad827d53a8ab38cf2023-12-03T14:13:51ZengMDPI AGMolecules1420-30492022-06-012713421410.3390/molecules27134214Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and PitfallsNorbert W. Lutz0Monique Bernard1CRMBM, CNRS, Aix-Marseille University, 13005 Marseille, FranceCRMBM, CNRS, Aix-Marseille University, 13005 Marseille, FranceThis is a broad overview and critical review of a particular group of closely related ex vivo and in vitro metabolic NMR spectroscopic methods. The scope of interest comprises studies of cultured cells and excised tissue, either intact or after physicochemical extraction of metabolites. Our detailed discussion includes pitfalls that have led to erroneous statements in the published literature, some of which may cause serious problems in metabolic and biological interpretation of results. To cover a wide range of work from relevant research areas, we consider not only the most recent achievements in the field, but also techniques that proved to be valid and successful in the past, although they may not have generated a very significant number of papers more recently. Thus, this comparative review also aims at providing background information useful for judiciously choosing between the metabolic ex vivo/in vitro NMR methods presented. Finally, the methods of interest are discussed in the context of, and in relation to, other metabolic analysis protocols such as HR-MAS and cell perfusion NMR, as well as the mass spectrometry approach.https://www.mdpi.com/1420-3049/27/13/4214in vitro NMR spectroscopytissuecellsmetabolismextractsheteronuclei |
| spellingShingle | Norbert W. Lutz Monique Bernard Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls Molecules in vitro NMR spectroscopy tissue cells metabolism extracts heteronuclei |
| title | Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls |
| title_full | Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls |
| title_fullStr | Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls |
| title_full_unstemmed | Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls |
| title_short | Methodological Developments for Metabolic NMR Spectroscopy from Cultured Cells to Tissue Extracts: Achievements, Progress and Pitfalls |
| title_sort | methodological developments for metabolic nmr spectroscopy from cultured cells to tissue extracts achievements progress and pitfalls |
| topic | in vitro NMR spectroscopy tissue cells metabolism extracts heteronuclei |
| url | https://www.mdpi.com/1420-3049/27/13/4214 |
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