Thymol Edible Coating Controls Postharvest Anthracnose by Regulating the Synthesis Pathway of Okra Lignin
Okra has received extensive attention due to its high nutritional value and remarkable functional characteristics, but postharvest diseases have severely limited its application. It is important to further explore the methods and potential methods to control the postharvest diseases of okra. In this...
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MDPI AG
2023-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/2/395 |
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| author | Qinqiu Zhang Zhuwei Wang Yinglu Li Xinzhi Liu Lang Liu Jing Yan Xinjie Hu Wen Qin |
| author_facet | Qinqiu Zhang Zhuwei Wang Yinglu Li Xinzhi Liu Lang Liu Jing Yan Xinjie Hu Wen Qin |
| author_sort | Qinqiu Zhang |
| collection | DOAJ |
| description | Okra has received extensive attention due to its high nutritional value and remarkable functional characteristics, but postharvest diseases have severely limited its application. It is important to further explore the methods and potential methods to control the postharvest diseases of okra. In this study, <i>Colletotrichum fioriniae</i> is the major pathogen that causes okra anthracnose, which can be isolated from naturally decaying okra. The pathogenicity of <i>C. fioriniae</i> against okra was preliminarily verified, and the related biological characteristics were explored. At the same time, an observational study was conducted to investigate the in vitro antifungal effect of thymol edible coating (TKL) on <i>C. fioriniae</i>. After culturing at 28 °C for 5 days, it was found that TKL showed an obvious growth inhibition effect on <i>C. fioriniae</i>. The concentration for 50% of the maximal effect was 95.10 mg/L, and the minimum inhibitory concentration was 1000 mg/L. In addition, it was found that thymol edible coating with a thymol concentration of 100 mg/L (TKL100) may cause different degrees of damage to the cell membrane, cell wall, and metabolism of <i>C. fioriniae</i>, thereby inhibiting the growth of hyphae and causing hyphal rupture. Refer to the results of the in vitro bacteriostatic experiment. Furthermore, the okra was sprayed with TKL100. It was found that the TKL100 coating could significantly inhibit the infection of <i>C. fioriniae</i> to okra, reduce the rate of brown spots and fold on the okra surface, and inhibit mycelium growth. In addition, the contents of total phenols and flavonoids of okra treated with TKL100 were higher than those of the control group. Meanwhile, the activities of phenylalaninammo-nialyase, cinnamic acid-4-hydroxylase, and 4-coumarate-CoA ligase in the lignin synthesis pathway were generally increased, especially after 6 days in a 28 °C incubator. The lignin content of TKL-W was the highest, reaching 65.62 ± 0.68 mg/g, which was 2.24 times of that of CK-W. Therefore, TKL may promote the synthesis of total phenols and flavonoids in okra, then stimulate the activity of key enzymes in the lignin synthesis pathway, and finally regulate the synthesis of lignin in okra. Thus, TKL could have a certain controlling effect on okra anthracnose. |
| first_indexed | 2024-03-09T12:42:39Z |
| format | Article |
| id | doaj.art-3c4ca572b4294c3ba265505d9f0f5072 |
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| language | English |
| last_indexed | 2024-03-09T12:42:39Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-3c4ca572b4294c3ba265505d9f0f50722023-11-30T22:15:55ZengMDPI AGFoods2304-81582023-01-0112239510.3390/foods12020395Thymol Edible Coating Controls Postharvest Anthracnose by Regulating the Synthesis Pathway of Okra LigninQinqiu Zhang0Zhuwei Wang1Yinglu Li2Xinzhi Liu3Lang Liu4Jing Yan5Xinjie Hu6Wen Qin7College of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, ChinaOkra has received extensive attention due to its high nutritional value and remarkable functional characteristics, but postharvest diseases have severely limited its application. It is important to further explore the methods and potential methods to control the postharvest diseases of okra. In this study, <i>Colletotrichum fioriniae</i> is the major pathogen that causes okra anthracnose, which can be isolated from naturally decaying okra. The pathogenicity of <i>C. fioriniae</i> against okra was preliminarily verified, and the related biological characteristics were explored. At the same time, an observational study was conducted to investigate the in vitro antifungal effect of thymol edible coating (TKL) on <i>C. fioriniae</i>. After culturing at 28 °C for 5 days, it was found that TKL showed an obvious growth inhibition effect on <i>C. fioriniae</i>. The concentration for 50% of the maximal effect was 95.10 mg/L, and the minimum inhibitory concentration was 1000 mg/L. In addition, it was found that thymol edible coating with a thymol concentration of 100 mg/L (TKL100) may cause different degrees of damage to the cell membrane, cell wall, and metabolism of <i>C. fioriniae</i>, thereby inhibiting the growth of hyphae and causing hyphal rupture. Refer to the results of the in vitro bacteriostatic experiment. Furthermore, the okra was sprayed with TKL100. It was found that the TKL100 coating could significantly inhibit the infection of <i>C. fioriniae</i> to okra, reduce the rate of brown spots and fold on the okra surface, and inhibit mycelium growth. In addition, the contents of total phenols and flavonoids of okra treated with TKL100 were higher than those of the control group. Meanwhile, the activities of phenylalaninammo-nialyase, cinnamic acid-4-hydroxylase, and 4-coumarate-CoA ligase in the lignin synthesis pathway were generally increased, especially after 6 days in a 28 °C incubator. The lignin content of TKL-W was the highest, reaching 65.62 ± 0.68 mg/g, which was 2.24 times of that of CK-W. Therefore, TKL may promote the synthesis of total phenols and flavonoids in okra, then stimulate the activity of key enzymes in the lignin synthesis pathway, and finally regulate the synthesis of lignin in okra. Thus, TKL could have a certain controlling effect on okra anthracnose.https://www.mdpi.com/2304-8158/12/2/395postharvest okra anthracnose<i>C. fioriniae</i>thymol edible coatinglignin synthesis pathway |
| spellingShingle | Qinqiu Zhang Zhuwei Wang Yinglu Li Xinzhi Liu Lang Liu Jing Yan Xinjie Hu Wen Qin Thymol Edible Coating Controls Postharvest Anthracnose by Regulating the Synthesis Pathway of Okra Lignin Foods postharvest okra anthracnose <i>C. fioriniae</i> thymol edible coating lignin synthesis pathway |
| title | Thymol Edible Coating Controls Postharvest Anthracnose by Regulating the Synthesis Pathway of Okra Lignin |
| title_full | Thymol Edible Coating Controls Postharvest Anthracnose by Regulating the Synthesis Pathway of Okra Lignin |
| title_fullStr | Thymol Edible Coating Controls Postharvest Anthracnose by Regulating the Synthesis Pathway of Okra Lignin |
| title_full_unstemmed | Thymol Edible Coating Controls Postharvest Anthracnose by Regulating the Synthesis Pathway of Okra Lignin |
| title_short | Thymol Edible Coating Controls Postharvest Anthracnose by Regulating the Synthesis Pathway of Okra Lignin |
| title_sort | thymol edible coating controls postharvest anthracnose by regulating the synthesis pathway of okra lignin |
| topic | postharvest okra anthracnose <i>C. fioriniae</i> thymol edible coating lignin synthesis pathway |
| url | https://www.mdpi.com/2304-8158/12/2/395 |
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