The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance
D-532 fertilization solution is generally used to replace the water or ovarian fluid during artificial reproductive practices in salmonids due to its ability to boost sperm motility and increase fertilization rates compared with natural activation media. However, the maintenance of ovarian fluid in...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/3/219 |
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| author | Giusy Rusco Michele Di Iorio Stefano Esposito Emanuele Antenucci Alessandra Roncarati Nicolaia Iaffaldano |
| author_facet | Giusy Rusco Michele Di Iorio Stefano Esposito Emanuele Antenucci Alessandra Roncarati Nicolaia Iaffaldano |
| author_sort | Giusy Rusco |
| collection | DOAJ |
| description | D-532 fertilization solution is generally used to replace the water or ovarian fluid during artificial reproductive practices in salmonids due to its ability to boost sperm motility and increase fertilization rates compared with natural activation media. However, the maintenance of ovarian fluid in a reproductive microenvironment gives it the advantage of protecting the eggs from potential harmful factors from the external environment and simplifying the field operations related to its removal when D-532 is used alone. In light of this, the aim of the present study was to investigate in vitro, for the first time, the effect of ovarian fluid (OF 100%) on post-thaw sperm swimming performance of Mediterranean trout, comparing it with D-532 and a mixed solution of 50% D-532 and 50% ovarian fluid (OF 50%). The percentage of motile spermatozoa and movement duration was significantly increased in OF 100% and OF 50% compared with D-532. Sperm velocity was higher in D-532, but significant differences were recorded only with OF 100%. In conclusion, these results suggest that the presence of ovarian fluid alone or in combination with D-532 in an artificial microenvironment of reproduction represents a key factor in potentially increasing fertilization success when the frozen semen of Mediterranean brown trout is used. |
| first_indexed | 2024-03-11T05:46:53Z |
| format | Article |
| id | doaj.art-3c4d1a132c604c03baa60db3952ae1a8 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T05:46:53Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-3c4d1a132c604c03baa60db3952ae1a82023-11-17T14:20:57ZengMDPI AGVeterinary Sciences2306-73812023-03-0110321910.3390/vetsci10030219The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming PerformanceGiusy Rusco0Michele Di Iorio1Stefano Esposito2Emanuele Antenucci3Alessandra Roncarati4Nicolaia Iaffaldano5Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, ItalyDepartment of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, ItalyMediterranean Trout Research Group “MTRG”, 42032 Ventasso (RE), ItalyDepartment of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62024 Matelica, ItalyDepartment of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, ItalyD-532 fertilization solution is generally used to replace the water or ovarian fluid during artificial reproductive practices in salmonids due to its ability to boost sperm motility and increase fertilization rates compared with natural activation media. However, the maintenance of ovarian fluid in a reproductive microenvironment gives it the advantage of protecting the eggs from potential harmful factors from the external environment and simplifying the field operations related to its removal when D-532 is used alone. In light of this, the aim of the present study was to investigate in vitro, for the first time, the effect of ovarian fluid (OF 100%) on post-thaw sperm swimming performance of Mediterranean trout, comparing it with D-532 and a mixed solution of 50% D-532 and 50% ovarian fluid (OF 50%). The percentage of motile spermatozoa and movement duration was significantly increased in OF 100% and OF 50% compared with D-532. Sperm velocity was higher in D-532, but significant differences were recorded only with OF 100%. In conclusion, these results suggest that the presence of ovarian fluid alone or in combination with D-532 in an artificial microenvironment of reproduction represents a key factor in potentially increasing fertilization success when the frozen semen of Mediterranean brown trout is used.https://www.mdpi.com/2306-7381/10/3/219fertilization mediumfrozen semenartificial reproductive microenvironmentconservation project |
| spellingShingle | Giusy Rusco Michele Di Iorio Stefano Esposito Emanuele Antenucci Alessandra Roncarati Nicolaia Iaffaldano The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance Veterinary Sciences fertilization medium frozen semen artificial reproductive microenvironment conservation project |
| title | The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance |
| title_full | The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance |
| title_fullStr | The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance |
| title_full_unstemmed | The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance |
| title_short | The Use of Ovarian Fluid as Natural Fertilization Medium for Cryopreserved Semen in Mediterranean Brown Trout: The Effects on Sperm Swimming Performance |
| title_sort | use of ovarian fluid as natural fertilization medium for cryopreserved semen in mediterranean brown trout the effects on sperm swimming performance |
| topic | fertilization medium frozen semen artificial reproductive microenvironment conservation project |
| url | https://www.mdpi.com/2306-7381/10/3/219 |
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