Active and passive immunization with rHyr1p-N protects mice against hematogenously disseminated candidiasis.
We previously reported that Candida albicans cell surface protein Hyr1 encodes a phagocyte killing resistance factor and active vaccination with a recombinant N-terminus of Hyr1 protein (rHyr1p-N), significantly protects immunocompetent mice from disseminated candidiasis. Here we report the marked e...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3189951?pdf=render |
| _version_ | 1818153475651928064 |
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| author | Guanpingsheng Luo Ashraf S Ibrahim Samuel W French John E Edwards Yue Fu |
| author_facet | Guanpingsheng Luo Ashraf S Ibrahim Samuel W French John E Edwards Yue Fu |
| author_sort | Guanpingsheng Luo |
| collection | DOAJ |
| description | We previously reported that Candida albicans cell surface protein Hyr1 encodes a phagocyte killing resistance factor and active vaccination with a recombinant N-terminus of Hyr1 protein (rHyr1p-N), significantly protects immunocompetent mice from disseminated candidiasis. Here we report the marked efficacy of rHyr1p-N vaccine on improving the survival and reducing the fungal burden of disseminated candidiasis in both immunocompetent and immunocompromised mice using the FDA-approved adjuvant, alum. Importantly, we also show that pooled rabbit anti-Hyr1p polyclonal antibodies raised against 8 different peptide regions of rHyr1p-N protected mice in a hematogenously disseminated candidiasis model, raising the possibility of developing a successful passive immunotherapy strategy to treat this disease. Our data suggest that the rabbit anti-Hyr1p antibodies directly neutralized the Hyr1p virulence function, rather than enhanced opsonophagocytosis for subsequent killing by neutrophil in vitro. Finally, the rHyr1p-N vaccine was protective against non-albicans Candida spp. These preclinical data demonstrate that rHyr1p-N is likely to be a novel target for developing both active and passive immunization strategies against Candida infections. |
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| format | Article |
| id | doaj.art-3c53ec7d8e7f4063930a62b4f2681f38 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T14:11:12Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-3c53ec7d8e7f4063930a62b4f2681f382022-12-22T01:03:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2590910.1371/journal.pone.0025909Active and passive immunization with rHyr1p-N protects mice against hematogenously disseminated candidiasis.Guanpingsheng LuoAshraf S IbrahimSamuel W FrenchJohn E EdwardsYue FuWe previously reported that Candida albicans cell surface protein Hyr1 encodes a phagocyte killing resistance factor and active vaccination with a recombinant N-terminus of Hyr1 protein (rHyr1p-N), significantly protects immunocompetent mice from disseminated candidiasis. Here we report the marked efficacy of rHyr1p-N vaccine on improving the survival and reducing the fungal burden of disseminated candidiasis in both immunocompetent and immunocompromised mice using the FDA-approved adjuvant, alum. Importantly, we also show that pooled rabbit anti-Hyr1p polyclonal antibodies raised against 8 different peptide regions of rHyr1p-N protected mice in a hematogenously disseminated candidiasis model, raising the possibility of developing a successful passive immunotherapy strategy to treat this disease. Our data suggest that the rabbit anti-Hyr1p antibodies directly neutralized the Hyr1p virulence function, rather than enhanced opsonophagocytosis for subsequent killing by neutrophil in vitro. Finally, the rHyr1p-N vaccine was protective against non-albicans Candida spp. These preclinical data demonstrate that rHyr1p-N is likely to be a novel target for developing both active and passive immunization strategies against Candida infections.http://europepmc.org/articles/PMC3189951?pdf=render |
| spellingShingle | Guanpingsheng Luo Ashraf S Ibrahim Samuel W French John E Edwards Yue Fu Active and passive immunization with rHyr1p-N protects mice against hematogenously disseminated candidiasis. PLoS ONE |
| title | Active and passive immunization with rHyr1p-N protects mice against hematogenously disseminated candidiasis. |
| title_full | Active and passive immunization with rHyr1p-N protects mice against hematogenously disseminated candidiasis. |
| title_fullStr | Active and passive immunization with rHyr1p-N protects mice against hematogenously disseminated candidiasis. |
| title_full_unstemmed | Active and passive immunization with rHyr1p-N protects mice against hematogenously disseminated candidiasis. |
| title_short | Active and passive immunization with rHyr1p-N protects mice against hematogenously disseminated candidiasis. |
| title_sort | active and passive immunization with rhyr1p n protects mice against hematogenously disseminated candidiasis |
| url | http://europepmc.org/articles/PMC3189951?pdf=render |
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