Plant regeneration from callus culture of vetiver (Vetiveria zizanioides Nash)
The present research aimed to establish cell suspension culture of vetiver (Vetiveria zizanioides Nash) from Surat Thani germplasm source and efficient plant regeneration from callus derived from such cultures. Cell suspension cultures were established from calli derived from inflorescence of vetive...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2003-09-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
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| Online Access: | http://www.sjst.psu.ac.th/journal/25-5-pdf/10callus.pdf |
| _version_ | 1818328702508859392 |
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| author | Somporn Prasertsongskun |
| author_facet | Somporn Prasertsongskun |
| author_sort | Somporn Prasertsongskun |
| collection | DOAJ |
| description | The present research aimed to establish cell suspension culture of vetiver (Vetiveria zizanioides Nash) from Surat Thani germplasm source and efficient plant regeneration from callus derived from such cultures. Cell suspension cultures were established from calli derived from inflorescence of vetiver. Optimum cell proliferation occurred in liquid N6 medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 10 mM proline. The cell suspension formed the highest small colonies when plated on solid MS medium containing 0.45 μM 2,4-D. After subsequent transfer to regeneration medium (MS free medium) 65% of plantlets were obtained. |
| first_indexed | 2024-12-13T12:36:22Z |
| format | Article |
| id | doaj.art-3c54a722c6e748469a036212d8cfdeab |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-13T12:36:22Z |
| publishDate | 2003-09-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-3c54a722c6e748469a036212d8cfdeab2022-12-21T23:45:51ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952003-09-01255637642Plant regeneration from callus culture of vetiver (Vetiveria zizanioides Nash)Somporn PrasertsongskunThe present research aimed to establish cell suspension culture of vetiver (Vetiveria zizanioides Nash) from Surat Thani germplasm source and efficient plant regeneration from callus derived from such cultures. Cell suspension cultures were established from calli derived from inflorescence of vetiver. Optimum cell proliferation occurred in liquid N6 medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 10 mM proline. The cell suspension formed the highest small colonies when plated on solid MS medium containing 0.45 μM 2,4-D. After subsequent transfer to regeneration medium (MS free medium) 65% of plantlets were obtained.http://www.sjst.psu.ac.th/journal/25-5-pdf/10callus.pdfregenerationcallusvetiverVetiveria zizanioides |
| spellingShingle | Somporn Prasertsongskun Plant regeneration from callus culture of vetiver (Vetiveria zizanioides Nash) Songklanakarin Journal of Science and Technology (SJST) regeneration callus vetiver Vetiveria zizanioides |
| title | Plant regeneration from callus culture of vetiver (Vetiveria zizanioides Nash) |
| title_full | Plant regeneration from callus culture of vetiver (Vetiveria zizanioides Nash) |
| title_fullStr | Plant regeneration from callus culture of vetiver (Vetiveria zizanioides Nash) |
| title_full_unstemmed | Plant regeneration from callus culture of vetiver (Vetiveria zizanioides Nash) |
| title_short | Plant regeneration from callus culture of vetiver (Vetiveria zizanioides Nash) |
| title_sort | plant regeneration from callus culture of vetiver vetiveria zizanioides nash |
| topic | regeneration callus vetiver Vetiveria zizanioides |
| url | http://www.sjst.psu.ac.th/journal/25-5-pdf/10callus.pdf |
| work_keys_str_mv | AT sompornprasertsongskun plantregenerationfromcalluscultureofvetivervetiveriazizanioidesnash |