Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.)
Rheum ribes L. is a perennial wild medicinal plant. In this study, the different explants types (hypocotyl and cotyledon) taken from 7-days-old plantlets were cultured in MS (Murashige and Skoog) media supplemented with 0.0, 1.0 and 2.0 mg L-1 of 6-Benzylaminopurine (BAP) with 0.0 and 1.0 mg L-1 o...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siirt University
2017-10-01
|
| Series: | Türkiye Tarımsal Araştırmalar Dergisi |
| Subjects: | |
| Online Access: | http://dergipark.gov.tr/tutad/issue/31029/323431 |
| _version_ | 1797923744039043072 |
|---|---|
| author | Burcu TUNCER Büşra GÜNSAN |
| author_facet | Burcu TUNCER Büşra GÜNSAN |
| author_sort | Burcu TUNCER |
| collection | DOAJ |
| description | Rheum ribes L. is a perennial wild medicinal plant. In this study, the different explants types (hypocotyl and
cotyledon) taken from 7-days-old plantlets were cultured in MS (Murashige and Skoog) media supplemented with 0.0, 1.0
and 2.0 mg L-1 of 6-Benzylaminopurine (BAP) with 0.0 and 1.0 mg L-1 of Indole-3-butyric acid (IBA). Hypocotyl explants
were found to be more successful than cotyledon explants in terms of shoot and callus formation. The highest number of
shoots per explant (4.0 shoot explant-1) was obtained from MS medium supplemented with 2 mg L-1 BAP and 1 mg L-1 IBA
(MS6) from the hypocotyl explants. In terms of the rate of callus formation (%), MS4 (MS + 1 mg L-1 BAP + 1 mg L-1 IBA)
(88.8%) and MS6 medium (MS + 2 mg L-1 BAP + 1 mg L-1 IBA) (83.3%) were found to be more successful than the
hypocotyl explants. |
| first_indexed | 2024-04-10T14:51:00Z |
| format | Article |
| id | doaj.art-3c57acc43cc24ac89c39749d7f597d80 |
| institution | Directory Open Access Journal |
| issn | 2148-2306 2528-858X |
| language | English |
| last_indexed | 2024-04-10T14:51:00Z |
| publishDate | 2017-10-01 |
| publisher | Siirt University |
| record_format | Article |
| series | Türkiye Tarımsal Araştırmalar Dergisi |
| spelling | doaj.art-3c57acc43cc24ac89c39749d7f597d802023-02-15T16:07:36ZengSiirt UniversityTürkiye Tarımsal Araştırmalar Dergisi2148-23062528-858X2017-10-014329630110.19159/tutad.323431Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.)Burcu TUNCER0Büşra GÜNSAN1Yüzüncü Yıl Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, Van, TÜRKİYEVan Yüzüncü Yıl Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, Van, TÜRKİYERheum ribes L. is a perennial wild medicinal plant. In this study, the different explants types (hypocotyl and cotyledon) taken from 7-days-old plantlets were cultured in MS (Murashige and Skoog) media supplemented with 0.0, 1.0 and 2.0 mg L-1 of 6-Benzylaminopurine (BAP) with 0.0 and 1.0 mg L-1 of Indole-3-butyric acid (IBA). Hypocotyl explants were found to be more successful than cotyledon explants in terms of shoot and callus formation. The highest number of shoots per explant (4.0 shoot explant-1) was obtained from MS medium supplemented with 2 mg L-1 BAP and 1 mg L-1 IBA (MS6) from the hypocotyl explants. In terms of the rate of callus formation (%), MS4 (MS + 1 mg L-1 BAP + 1 mg L-1 IBA) (88.8%) and MS6 medium (MS + 2 mg L-1 BAP + 1 mg L-1 IBA) (83.3%) were found to be more successful than the hypocotyl explants.http://dergipark.gov.tr/tutad/issue/31029/323431Rheum ribes L.in vitro regenerationcallusshoot |
| spellingShingle | Burcu TUNCER Büşra GÜNSAN Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.) Türkiye Tarımsal Araştırmalar Dergisi Rheum ribes L. in vitro regeneration callus shoot |
| title | Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.) |
| title_full | Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.) |
| title_fullStr | Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.) |
| title_full_unstemmed | Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.) |
| title_short | Research on Regeneration Via Tissue Culture on Wild Rhubarb (Rheum ribes L.) |
| title_sort | research on regeneration via tissue culture on wild rhubarb rheum ribes l |
| topic | Rheum ribes L. in vitro regeneration callus shoot |
| url | http://dergipark.gov.tr/tutad/issue/31029/323431 |
| work_keys_str_mv | AT burcutuncer researchonregenerationviatissuecultureonwildrhubarbrheumribesl AT busragunsan researchonregenerationviatissuecultureonwildrhubarbrheumribesl |