Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing
Abstract Background Rats (Rattus spp.) invaded most of the world as stowaways including some that carried the rat lungworm, Angiostrongylus cantonensis, the cause of eosinophilic meningoencephalitis in humans and other warm-blooded animals. A high genetic diversity of A. cantonensis based on short m...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3491-y |
| _version_ | 1818527555588718592 |
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| author | Barbora Červená David Modrý Barbora Fecková Kristýna Hrazdilová Pilar Foronda Aron Martin Alonso Rogan Lee John Walker Chris N. Niebuhr Richard Malik Jan Šlapeta |
| author_facet | Barbora Červená David Modrý Barbora Fecková Kristýna Hrazdilová Pilar Foronda Aron Martin Alonso Rogan Lee John Walker Chris N. Niebuhr Richard Malik Jan Šlapeta |
| author_sort | Barbora Červená |
| collection | DOAJ |
| description | Abstract Background Rats (Rattus spp.) invaded most of the world as stowaways including some that carried the rat lungworm, Angiostrongylus cantonensis, the cause of eosinophilic meningoencephalitis in humans and other warm-blooded animals. A high genetic diversity of A. cantonensis based on short mitochondrial DNA regions is reported from Southeast Asia. However, the identity of invasive A. cantonensis is known for only a minority of countries. The affordability of next-generation sequencing for characterisation of A. cantonensis genomes should enable new insights into rat lung worm invasion and parasite identification in experimental studies. Methods Genomic DNA from morphologically verified A. cantonensis (two laboratory-maintained strains and two field isolates) was sequenced using low coverage whole genome sequencing. The complete mitochondrial genome was assembled and compared to published A. cantonensis and Angiostrongylus malaysiensis sequences. To determine if the commonly sequenced partial cox1 can unequivocally identify A. cantonensis genetic lineages, the diversity of cox1 was re-evaluated in the context of the publicly available cox1 sequences and the entire mitochondrial genomes. Published experimental studies available in Web of Science were systematically reviewed to reveal published identities of A. cantonensis used in experimental studies. Results New A. cantonensis mitochondrial genomes from Sydney (Australia), Hawaii (USA), Canary Islands (Spain) and Fatu Hiva (French Polynesia), were assembled from next-generation sequencing data. Comparison of A. cantonensis mitochondrial genomes from outside of Southeast Asia showed low genetic diversity (0.02–1.03%) within a single lineage of A. cantonensis. Both cox1 and cox2 were considered the preferred markers for A. cantonensis haplotype identification. Systematic review revealed that unequivocal A. cantonensis identification of strains used in experimental studies is hindered by absence of their genetic and geographical identity. Conclusions Low coverage whole genome sequencing provides data enabling standardised identification of A. cantonensis laboratory strains and field isolates. The phenotype of invasive A. cantonensis, such as the capacity to establish in new territories, has a strong genetic component, as the A. cantonensis found outside of the original endemic area are genetically uniform. It is imperative that the genotype of A. cantonensis strains maintained in laboratories and used in experimental studies is unequivocally characterised. |
| first_indexed | 2024-12-11T06:37:38Z |
| format | Article |
| id | doaj.art-3c5e834d27804baca581060ce3505bd1 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-11T06:37:38Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-3c5e834d27804baca581060ce3505bd12022-12-22T01:17:19ZengBMCParasites & Vectors1756-33052019-05-0112111310.1186/s13071-019-3491-yLow diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencingBarbora Červená0David Modrý1Barbora Fecková2Kristýna Hrazdilová3Pilar Foronda4Aron Martin Alonso5Rogan Lee6John Walker7Chris N. Niebuhr8Richard Malik9Jan Šlapeta10Sydney School of Veterinary Science, University of SydneyDepartment of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences BrnoDepartment of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences BrnoCEITEC VFU, University of Veterinary and Pharmaceutical Sciences BrnoInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La LagunaInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La LagunaWestmead Clinical School, University of SydneyMarie Bashir Institute for infectious Diseases and Biosecurity, University of SydneyUSDA-APHIS-WS, National Wildlife Research CenterCentre for Veterinary Education, University of SydneySydney School of Veterinary Science, University of SydneyAbstract Background Rats (Rattus spp.) invaded most of the world as stowaways including some that carried the rat lungworm, Angiostrongylus cantonensis, the cause of eosinophilic meningoencephalitis in humans and other warm-blooded animals. A high genetic diversity of A. cantonensis based on short mitochondrial DNA regions is reported from Southeast Asia. However, the identity of invasive A. cantonensis is known for only a minority of countries. The affordability of next-generation sequencing for characterisation of A. cantonensis genomes should enable new insights into rat lung worm invasion and parasite identification in experimental studies. Methods Genomic DNA from morphologically verified A. cantonensis (two laboratory-maintained strains and two field isolates) was sequenced using low coverage whole genome sequencing. The complete mitochondrial genome was assembled and compared to published A. cantonensis and Angiostrongylus malaysiensis sequences. To determine if the commonly sequenced partial cox1 can unequivocally identify A. cantonensis genetic lineages, the diversity of cox1 was re-evaluated in the context of the publicly available cox1 sequences and the entire mitochondrial genomes. Published experimental studies available in Web of Science were systematically reviewed to reveal published identities of A. cantonensis used in experimental studies. Results New A. cantonensis mitochondrial genomes from Sydney (Australia), Hawaii (USA), Canary Islands (Spain) and Fatu Hiva (French Polynesia), were assembled from next-generation sequencing data. Comparison of A. cantonensis mitochondrial genomes from outside of Southeast Asia showed low genetic diversity (0.02–1.03%) within a single lineage of A. cantonensis. Both cox1 and cox2 were considered the preferred markers for A. cantonensis haplotype identification. Systematic review revealed that unequivocal A. cantonensis identification of strains used in experimental studies is hindered by absence of their genetic and geographical identity. Conclusions Low coverage whole genome sequencing provides data enabling standardised identification of A. cantonensis laboratory strains and field isolates. The phenotype of invasive A. cantonensis, such as the capacity to establish in new territories, has a strong genetic component, as the A. cantonensis found outside of the original endemic area are genetically uniform. It is imperative that the genotype of A. cantonensis strains maintained in laboratories and used in experimental studies is unequivocally characterised.http://link.springer.com/article/10.1186/s13071-019-3491-yRat lungwormMitochondrial genomeGenetic diversityInvasive speciesNext-generation sequencingRat lungworm |
| spellingShingle | Barbora Červená David Modrý Barbora Fecková Kristýna Hrazdilová Pilar Foronda Aron Martin Alonso Rogan Lee John Walker Chris N. Niebuhr Richard Malik Jan Šlapeta Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing Parasites & Vectors Rat lungworm Mitochondrial genome Genetic diversity Invasive species Next-generation sequencing Rat lungworm |
| title | Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing |
| title_full | Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing |
| title_fullStr | Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing |
| title_full_unstemmed | Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing |
| title_short | Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing |
| title_sort | low diversity of angiostrongylus cantonensis complete mitochondrial dna sequences from australia hawaii french polynesia and the canary islands revealed using whole genome next generation sequencing |
| topic | Rat lungworm Mitochondrial genome Genetic diversity Invasive species Next-generation sequencing Rat lungworm |
| url | http://link.springer.com/article/10.1186/s13071-019-3491-y |
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