Cutinolytic Esterases are Induced by Growth of the Fungus Trichoderma harzianum on Glyceryl Monostearate in Solid-State Fermentation
Cutinolytic esterase are enzymes utilized in a wide variety of industrial applications, and they are capable of degrading emerging environmental pollutants. Due to the application and importance of these enzymes, it is crucial to develop an efficient method for cutinase production using a cost-effec...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2023-10-01
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| Series: | BioResources |
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| Online Access: | https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/23024 |
| _version_ | 1797643253568241664 |
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| author | Victoria Conde-Avila Octavio Loera-Corral Ruben Díaz Carmen Sanchez |
| author_facet | Victoria Conde-Avila Octavio Loera-Corral Ruben Díaz Carmen Sanchez |
| author_sort | Victoria Conde-Avila |
| collection | DOAJ |
| description | Cutinolytic esterase are enzymes utilized in a wide variety of industrial applications, and they are capable of degrading emerging environmental pollutants. Due to the application and importance of these enzymes, it is crucial to develop an efficient method for cutinase production using a cost-effective inductor and an efficient microbial production system. In this work, the growth and cutinolytic esterase production of Trichoderma harzianum were evaluated in glucose-yeast extract media containing different glyceryl monostearate (GMS) concentrations (1, 3, and 5 g/L). It was used as inducer in solid-state fermentation. A medium lacking GMS was used as control. Biomass production and enzyme productivity were higher in inducer-added (1 g/L) medium than in the control medium. T. harzianum produced constitutive and inducible cutinolytic esterase, in which production was enhanced by GMS. In GMS-added cultures, two bands with cutinolytic esterase activity (60 and 150 kDa approximately) were observed by zymography, which were not observed in control culture. GMS represents a promising inducer for cutinolytic esterase production by fungi. This research represents the first approach for the study of cutinolytic esterase production using a synthetic molecule as an inducer. |
| first_indexed | 2024-03-11T14:12:03Z |
| format | Article |
| id | doaj.art-3c64dfae33e248ccbe844d31bfd3a9f6 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-03-11T14:12:03Z |
| publishDate | 2023-10-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-3c64dfae33e248ccbe844d31bfd3a9f62023-11-01T17:16:52ZengNorth Carolina State UniversityBioResources1930-21262023-10-01184851585271118Cutinolytic Esterases are Induced by Growth of the Fungus Trichoderma harzianum on Glyceryl Monostearate in Solid-State FermentationVictoria Conde-Avila0Octavio Loera-Corral1Ruben Díaz2Carmen Sanchez3Universidad Autónoma de Occidente, Los Mochis, Sinaloa, C.P. 81223, MexicoUniversidad Autónoma Metropolitana-Unidad Iztapalapa, Iztapalapa, C.P. 09340, Mexico, City, MexicoLaboratory of Biotechnology, Research Centre for Biological Sciences, Universidad Autónoma de Tlaxcala, Ixtacuixtla, Tlaxcala, C.P. 90062, MexicoLaboratory of Biotechnology, Research Centre for Biological Sciences, Universidad Autónoma de Tlaxcala, Ixtacuixtla, Tlaxcala, C.P. 90062, MexicoCutinolytic esterase are enzymes utilized in a wide variety of industrial applications, and they are capable of degrading emerging environmental pollutants. Due to the application and importance of these enzymes, it is crucial to develop an efficient method for cutinase production using a cost-effective inductor and an efficient microbial production system. In this work, the growth and cutinolytic esterase production of Trichoderma harzianum were evaluated in glucose-yeast extract media containing different glyceryl monostearate (GMS) concentrations (1, 3, and 5 g/L). It was used as inducer in solid-state fermentation. A medium lacking GMS was used as control. Biomass production and enzyme productivity were higher in inducer-added (1 g/L) medium than in the control medium. T. harzianum produced constitutive and inducible cutinolytic esterase, in which production was enhanced by GMS. In GMS-added cultures, two bands with cutinolytic esterase activity (60 and 150 kDa approximately) were observed by zymography, which were not observed in control culture. GMS represents a promising inducer for cutinolytic esterase production by fungi. This research represents the first approach for the study of cutinolytic esterase production using a synthetic molecule as an inducer.https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/23024biomass productioncutinolytic esterase activityglyceryl monostearatesolid-state fermentationtrichoderma harzianum |
| spellingShingle | Victoria Conde-Avila Octavio Loera-Corral Ruben Díaz Carmen Sanchez Cutinolytic Esterases are Induced by Growth of the Fungus Trichoderma harzianum on Glyceryl Monostearate in Solid-State Fermentation BioResources biomass production cutinolytic esterase activity glyceryl monostearate solid-state fermentation trichoderma harzianum |
| title | Cutinolytic Esterases are Induced by Growth of the Fungus Trichoderma harzianum on Glyceryl Monostearate in Solid-State Fermentation |
| title_full | Cutinolytic Esterases are Induced by Growth of the Fungus Trichoderma harzianum on Glyceryl Monostearate in Solid-State Fermentation |
| title_fullStr | Cutinolytic Esterases are Induced by Growth of the Fungus Trichoderma harzianum on Glyceryl Monostearate in Solid-State Fermentation |
| title_full_unstemmed | Cutinolytic Esterases are Induced by Growth of the Fungus Trichoderma harzianum on Glyceryl Monostearate in Solid-State Fermentation |
| title_short | Cutinolytic Esterases are Induced by Growth of the Fungus Trichoderma harzianum on Glyceryl Monostearate in Solid-State Fermentation |
| title_sort | cutinolytic esterases are induced by growth of the fungus trichoderma harzianum on glyceryl monostearate in solid state fermentation |
| topic | biomass production cutinolytic esterase activity glyceryl monostearate solid-state fermentation trichoderma harzianum |
| url | https://ojs.cnr.ncsu.edu/index.php/BRJ/article/view/23024 |
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