GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential
GDP-mannose 3,5-epimerase (GM35E, GME) belongs to the short-chain dehydrogenase/reductase (SDR) protein superfamily and catalyses the conversion of GDP-d-mannose towards GDP-l-galactose. Although the overall reaction seems relatively simple (a double epimerization), the enzyme needs to orchestrate a...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-01-01
|
| Series: | Frontiers in Molecular Biosciences |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.784142/full |
| _version_ | 1798035896913625088 |
|---|---|
| author | Koen Beerens Ophelia Gevaert Tom Desmet |
| author_facet | Koen Beerens Ophelia Gevaert Tom Desmet |
| author_sort | Koen Beerens |
| collection | DOAJ |
| description | GDP-mannose 3,5-epimerase (GM35E, GME) belongs to the short-chain dehydrogenase/reductase (SDR) protein superfamily and catalyses the conversion of GDP-d-mannose towards GDP-l-galactose. Although the overall reaction seems relatively simple (a double epimerization), the enzyme needs to orchestrate a complex set of chemical reactions, with no less than 6 catalysis steps (oxidation, 2x deprotonation, 2x protonation and reduction), to perform the double epimerization of GDP-mannose to GDP-l-galactose. The enzyme is involved in the biosynthesis of vitamin C in plants and lipopolysaccharide synthesis in bacteria. In this review, we provide a clear overview of these interesting epimerases, including the latest findings such as the recently characterized bacterial and thermostable GM35E representative and its mechanism revision but also focus on their industrial potential in rare sugar synthesis and glycorandomization. |
| first_indexed | 2024-04-11T21:04:42Z |
| format | Article |
| id | doaj.art-3c6918e22b3846fd98d9d5851aef115f |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-11T21:04:42Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-3c6918e22b3846fd98d9d5851aef115f2022-12-22T04:03:23ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-01-01810.3389/fmolb.2021.784142784142GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial PotentialKoen BeerensOphelia GevaertTom DesmetGDP-mannose 3,5-epimerase (GM35E, GME) belongs to the short-chain dehydrogenase/reductase (SDR) protein superfamily and catalyses the conversion of GDP-d-mannose towards GDP-l-galactose. Although the overall reaction seems relatively simple (a double epimerization), the enzyme needs to orchestrate a complex set of chemical reactions, with no less than 6 catalysis steps (oxidation, 2x deprotonation, 2x protonation and reduction), to perform the double epimerization of GDP-mannose to GDP-l-galactose. The enzyme is involved in the biosynthesis of vitamin C in plants and lipopolysaccharide synthesis in bacteria. In this review, we provide a clear overview of these interesting epimerases, including the latest findings such as the recently characterized bacterial and thermostable GM35E representative and its mechanism revision but also focus on their industrial potential in rare sugar synthesis and glycorandomization.https://www.frontiersin.org/articles/10.3389/fmolb.2021.784142/fullepimeraseGDP-mannoseNS-SDRshort-chain dehydrogenase/reductase (SDR)NDP-sugar active SDR (NS-SDR)L-sugar |
| spellingShingle | Koen Beerens Ophelia Gevaert Tom Desmet GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential Frontiers in Molecular Biosciences epimerase GDP-mannose NS-SDR short-chain dehydrogenase/reductase (SDR) NDP-sugar active SDR (NS-SDR) L-sugar |
| title | GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential |
| title_full | GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential |
| title_fullStr | GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential |
| title_full_unstemmed | GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential |
| title_short | GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential |
| title_sort | gdp mannose 3 5 epimerase a view on structure mechanism and industrial potential |
| topic | epimerase GDP-mannose NS-SDR short-chain dehydrogenase/reductase (SDR) NDP-sugar active SDR (NS-SDR) L-sugar |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.784142/full |
| work_keys_str_mv | AT koenbeerens gdpmannose35epimeraseaviewonstructuremechanismandindustrialpotential AT opheliagevaert gdpmannose35epimeraseaviewonstructuremechanismandindustrialpotential AT tomdesmet gdpmannose35epimeraseaviewonstructuremechanismandindustrialpotential |