LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis
Abstract Background Long noncoding RNA (lncRNA), urothelial carcinoma-associated 1 (UCA1) is aberrantly expressed in multiple cancers and has been verified as an oncogene. However, the underlying mechanism of UCA1 in the development of gastric cancer is not fully understood. In the present study, we...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | Cellular & Molecular Biology Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s11658-021-00275-8 |
| _version_ | 1829135959827939328 |
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| author | An Yang Xin Liu Ping Liu Yunzhang Feng Hongbo Liu Shen Gao Limin Huo Xinyan Han Jurong Wang Wei Kong |
| author_facet | An Yang Xin Liu Ping Liu Yunzhang Feng Hongbo Liu Shen Gao Limin Huo Xinyan Han Jurong Wang Wei Kong |
| author_sort | An Yang |
| collection | DOAJ |
| description | Abstract Background Long noncoding RNA (lncRNA), urothelial carcinoma-associated 1 (UCA1) is aberrantly expressed in multiple cancers and has been verified as an oncogene. However, the underlying mechanism of UCA1 in the development of gastric cancer is not fully understood. In the present study, we aimed to identify how UCA1 promotes gastric cancer development. Methods The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data were used to analyze UCA1 and myosin VI (MYO6) expression in gastric cancer. Western blot and quantitative real-time PCR (QPCR) were performed to test the expression level of the UCA1/miR-145/MYO6 axis in gastric cancer cell lines and tissues. The roles of the UCA1/miR-145/MYO6 axis in gastric cancer in vitro and in vivo were investigated by CCK-8 assay, flow cytometry, siRNAs, immunohistochemistry, and a mouse xenograft model. The targeted relationship among UCA1, miR-145, and MYO6 was predicted using LncBase Predicted v.2 and TargetScan online software, and then verified by luciferase activity assay and RNA immunoprecipitation. Results UCA1 expression was higher but miR-145 expression was lower in gastric cancer cell lines or tissues, compared to the adjacent normal cell line or normal tissues. Function analysis verified that UCA1 promoted cell proliferation and inhibited cell apoptosis in the gastric cancer cells in vitro and in vivo. Mechanistically, UCA1 could bind directly to miR-145, and MYO6 was found to be a downstream target gene of miR-145. miR-145 mimics or MYO6 siRNAs could partly reverse the effect of UCA1 on gastric cancer cells. Conclusions UCA1 accelerated cell proliferation and inhibited cell apoptosis through sponging miR-145 to upregulate MYO6 expression in gastric cancer, indicating that the UCA1/miR-145/MYO6 axis may serve as a potential therapeutic target for gastric cancer. |
| first_indexed | 2024-12-14T18:33:56Z |
| format | Article |
| id | doaj.art-3c69949b0e62411789dcf3aa03494d1f |
| institution | Directory Open Access Journal |
| issn | 1425-8153 1689-1392 |
| language | English |
| last_indexed | 2024-12-14T18:33:56Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Cellular & Molecular Biology Letters |
| spelling | doaj.art-3c69949b0e62411789dcf3aa03494d1f2022-12-21T22:51:41ZengBMCCellular & Molecular Biology Letters1425-81531689-13922021-07-0126111810.1186/s11658-021-00275-8LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axisAn Yang0Xin Liu1Ping Liu2Yunzhang Feng3Hongbo Liu4Shen Gao5Limin Huo6Xinyan Han7Jurong Wang8Wei Kong9Handan Central HospitalAffiliated Hospital of Hebei University of EngineeringHandan Central HospitalHandan Central HospitalHandan Central HospitalHandan First HospitalHandan First HospitalHandan Central HospitalHandan Central HospitalHandan Central HospitalAbstract Background Long noncoding RNA (lncRNA), urothelial carcinoma-associated 1 (UCA1) is aberrantly expressed in multiple cancers and has been verified as an oncogene. However, the underlying mechanism of UCA1 in the development of gastric cancer is not fully understood. In the present study, we aimed to identify how UCA1 promotes gastric cancer development. Methods The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data were used to analyze UCA1 and myosin VI (MYO6) expression in gastric cancer. Western blot and quantitative real-time PCR (QPCR) were performed to test the expression level of the UCA1/miR-145/MYO6 axis in gastric cancer cell lines and tissues. The roles of the UCA1/miR-145/MYO6 axis in gastric cancer in vitro and in vivo were investigated by CCK-8 assay, flow cytometry, siRNAs, immunohistochemistry, and a mouse xenograft model. The targeted relationship among UCA1, miR-145, and MYO6 was predicted using LncBase Predicted v.2 and TargetScan online software, and then verified by luciferase activity assay and RNA immunoprecipitation. Results UCA1 expression was higher but miR-145 expression was lower in gastric cancer cell lines or tissues, compared to the adjacent normal cell line or normal tissues. Function analysis verified that UCA1 promoted cell proliferation and inhibited cell apoptosis in the gastric cancer cells in vitro and in vivo. Mechanistically, UCA1 could bind directly to miR-145, and MYO6 was found to be a downstream target gene of miR-145. miR-145 mimics or MYO6 siRNAs could partly reverse the effect of UCA1 on gastric cancer cells. Conclusions UCA1 accelerated cell proliferation and inhibited cell apoptosis through sponging miR-145 to upregulate MYO6 expression in gastric cancer, indicating that the UCA1/miR-145/MYO6 axis may serve as a potential therapeutic target for gastric cancer.https://doi.org/10.1186/s11658-021-00275-8Gastric cancerlncRNAUCA1miR-145MYO6 |
| spellingShingle | An Yang Xin Liu Ping Liu Yunzhang Feng Hongbo Liu Shen Gao Limin Huo Xinyan Han Jurong Wang Wei Kong LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis Cellular & Molecular Biology Letters Gastric cancer lncRNA UCA1 miR-145 MYO6 |
| title | LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis |
| title_full | LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis |
| title_fullStr | LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis |
| title_full_unstemmed | LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis |
| title_short | LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis |
| title_sort | lncrna uca1 promotes development of gastric cancer via the mir 145 myo6 axis |
| topic | Gastric cancer lncRNA UCA1 miR-145 MYO6 |
| url | https://doi.org/10.1186/s11658-021-00275-8 |
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