Human Cytomegalovirus Infection Changes the Pattern of Surface Markers of Small Extracellular Vesicles Isolated From First Trimester Placental Long-Term Histocultures
Extracellular vesicles (EVs) have increasingly been recognized as key players in a wide variety of physiological and pathological contexts, including during pregnancy. Notably, EVs appear both as possible biomarkers and as mediators involved in the communication of the placenta with the maternal and...
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.689122/full |
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| author | Mathilde Bergamelli Hélène Martin Mélinda Bénard Mélinda Bénard Jérôme Ausseil Jérôme Ausseil Jean-Michel Mansuy Ilse Hurbain Ilse Hurbain Maïlys Mouysset Marion Groussolles Marion Groussolles Marion Groussolles Géraldine Cartron Yann Tanguy le Gac Nathalie Moinard Nathalie Moinard Elsa Suberbielle Jacques Izopet Jacques Izopet Charlotte Tscherning Graça Raposo Graça Raposo Daniel Gonzalez-Dunia Gisela D’Angelo Cécile E. Malnou |
| author_facet | Mathilde Bergamelli Hélène Martin Mélinda Bénard Mélinda Bénard Jérôme Ausseil Jérôme Ausseil Jean-Michel Mansuy Ilse Hurbain Ilse Hurbain Maïlys Mouysset Marion Groussolles Marion Groussolles Marion Groussolles Géraldine Cartron Yann Tanguy le Gac Nathalie Moinard Nathalie Moinard Elsa Suberbielle Jacques Izopet Jacques Izopet Charlotte Tscherning Graça Raposo Graça Raposo Daniel Gonzalez-Dunia Gisela D’Angelo Cécile E. Malnou |
| author_sort | Mathilde Bergamelli |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) have increasingly been recognized as key players in a wide variety of physiological and pathological contexts, including during pregnancy. Notably, EVs appear both as possible biomarkers and as mediators involved in the communication of the placenta with the maternal and fetal sides. A better understanding of the physiological and pathological roles of EVs strongly depends on the development of adequate and reliable study models, specifically at the beginning of pregnancy where many adverse pregnancy outcomes have their origin. In this study, we describe the isolation of small EVs from a histoculture model of first trimester placental explants in normal conditions as well as upon infection by human cytomegalovirus. Using bead-based multiplex cytometry and electron microscopy combined with biochemical approaches, we characterized these small EVs and defined their associated markers and ultrastructure. We observed that infection led to changes in the expression level of several surface markers, without affecting the secretion and integrity of small EVs. Our findings lay the foundation for studying the functional role of EVs during early pregnancy, along with the identification of new predictive biomarkers for the severity and outcome of this congenital infection, which are still sorely lacking. |
| first_indexed | 2024-12-19T23:10:24Z |
| format | Article |
| id | doaj.art-3c6c7a0436584794b1360c2aaacebf18 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-19T23:10:24Z |
| publishDate | 2021-09-01 |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-3c6c7a0436584794b1360c2aaacebf182022-12-21T20:02:14ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-09-01910.3389/fcell.2021.689122689122Human Cytomegalovirus Infection Changes the Pattern of Surface Markers of Small Extracellular Vesicles Isolated From First Trimester Placental Long-Term HistoculturesMathilde Bergamelli0Hélène Martin1Mélinda Bénard2Mélinda Bénard3Jérôme Ausseil4Jérôme Ausseil5Jean-Michel Mansuy6Ilse Hurbain7Ilse Hurbain8Maïlys Mouysset9Marion Groussolles10Marion Groussolles11Marion Groussolles12Géraldine Cartron13Yann Tanguy le Gac14Nathalie Moinard15Nathalie Moinard16Elsa Suberbielle17Jacques Izopet18Jacques Izopet19Charlotte Tscherning20Graça Raposo21Graça Raposo22Daniel Gonzalez-Dunia23Gisela D’Angelo24Cécile E. Malnou25Institut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceService de Néonatalogie, CHU Toulouse, Hôpital des Enfants, Toulouse, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceLaboratoire de Biochimie, CHU Toulouse, Hôpital Rangueil, Toulouse, FranceLaboratoire de Virologie, CHU Toulouse, Hôpital Purpan, Toulouse, FranceCNRS UMR 144, Structure et Compartiments Membranaires, Institut Curie, Université Paris Sciences et Lettres, Paris, FranceCNRS UMR 144, Plateforme d’Imagerie Cellulaire et Tissulaire (PICT-IBiSA), Institut Curie, Université Paris Sciences et Lettres, Paris, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceService de Diagnostic Prénatal, CHU Toulouse, Hôpital Paule de Viguier, Toulouse, FranceINSERM UMR 1027, UPS, Equipe SPHERE Epidémiologie et Analyses en Santé Publique: Risques, Maladies Chroniques et Handicaps, Université de Toulouse, Toulouse, FranceService de Gynécologie Obstétrique, CHU Toulouse, Hôpital Paule de Viguier, Toulouse, FranceService de Gynécologie Obstétrique, CHU Toulouse, Hôpital Paule de Viguier, Toulouse, France0Développement Embryonnaire, Fertilité, Environnement (DEFE), INSERM UMR 1203, Université de Toulouse et Université de Montpellier, Montpellier, France1CECOS, Groupe d’Activité de Médecine de la Reproduction, CHU Toulouse, Hôpital Paule de Viguier, Toulouse, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceLaboratoire de Virologie, CHU Toulouse, Hôpital Purpan, Toulouse, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceCNRS UMR 144, Structure et Compartiments Membranaires, Institut Curie, Université Paris Sciences et Lettres, Paris, FranceCNRS UMR 144, Plateforme d’Imagerie Cellulaire et Tissulaire (PICT-IBiSA), Institut Curie, Université Paris Sciences et Lettres, Paris, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceCNRS UMR 144, Structure et Compartiments Membranaires, Institut Curie, Université Paris Sciences et Lettres, Paris, FranceInstitut Toulousain des Maladies Infectieuses et Inflammatoires (Infinity), INSERM, CNRS, UPS, Université de Toulouse, Toulouse, FranceExtracellular vesicles (EVs) have increasingly been recognized as key players in a wide variety of physiological and pathological contexts, including during pregnancy. Notably, EVs appear both as possible biomarkers and as mediators involved in the communication of the placenta with the maternal and fetal sides. A better understanding of the physiological and pathological roles of EVs strongly depends on the development of adequate and reliable study models, specifically at the beginning of pregnancy where many adverse pregnancy outcomes have their origin. In this study, we describe the isolation of small EVs from a histoculture model of first trimester placental explants in normal conditions as well as upon infection by human cytomegalovirus. Using bead-based multiplex cytometry and electron microscopy combined with biochemical approaches, we characterized these small EVs and defined their associated markers and ultrastructure. We observed that infection led to changes in the expression level of several surface markers, without affecting the secretion and integrity of small EVs. Our findings lay the foundation for studying the functional role of EVs during early pregnancy, along with the identification of new predictive biomarkers for the severity and outcome of this congenital infection, which are still sorely lacking.https://www.frontiersin.org/articles/10.3389/fcell.2021.689122/fullearly placentaextracellular vesiclescongenital infectionhuman cytomegalovirusplacental histoculture |
| spellingShingle | Mathilde Bergamelli Hélène Martin Mélinda Bénard Mélinda Bénard Jérôme Ausseil Jérôme Ausseil Jean-Michel Mansuy Ilse Hurbain Ilse Hurbain Maïlys Mouysset Marion Groussolles Marion Groussolles Marion Groussolles Géraldine Cartron Yann Tanguy le Gac Nathalie Moinard Nathalie Moinard Elsa Suberbielle Jacques Izopet Jacques Izopet Charlotte Tscherning Graça Raposo Graça Raposo Daniel Gonzalez-Dunia Gisela D’Angelo Cécile E. Malnou Human Cytomegalovirus Infection Changes the Pattern of Surface Markers of Small Extracellular Vesicles Isolated From First Trimester Placental Long-Term Histocultures Frontiers in Cell and Developmental Biology early placenta extracellular vesicles congenital infection human cytomegalovirus placental histoculture |
| title | Human Cytomegalovirus Infection Changes the Pattern of Surface Markers of Small Extracellular Vesicles Isolated From First Trimester Placental Long-Term Histocultures |
| title_full | Human Cytomegalovirus Infection Changes the Pattern of Surface Markers of Small Extracellular Vesicles Isolated From First Trimester Placental Long-Term Histocultures |
| title_fullStr | Human Cytomegalovirus Infection Changes the Pattern of Surface Markers of Small Extracellular Vesicles Isolated From First Trimester Placental Long-Term Histocultures |
| title_full_unstemmed | Human Cytomegalovirus Infection Changes the Pattern of Surface Markers of Small Extracellular Vesicles Isolated From First Trimester Placental Long-Term Histocultures |
| title_short | Human Cytomegalovirus Infection Changes the Pattern of Surface Markers of Small Extracellular Vesicles Isolated From First Trimester Placental Long-Term Histocultures |
| title_sort | human cytomegalovirus infection changes the pattern of surface markers of small extracellular vesicles isolated from first trimester placental long term histocultures |
| topic | early placenta extracellular vesicles congenital infection human cytomegalovirus placental histoculture |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.689122/full |
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