The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic Steatosis
Background: The microbiome has been shown in pre-clinical and epidemiological studies to be important in both the development and treatment of obesity and metabolic associated fatty liver disease (MAFLD). However, few studies have examined the role of the microbiome in the clinical response to calor...
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Frontiers Media S.A.
2021-07-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.718661/full |
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| author | Tien S. Dong Tien S. Dong Tien S. Dong Tien S. Dong Kayti Luu Kayti Luu Kayti Luu Kayti Luu Venu Lagishetty Venu Lagishetty Venu Lagishetty Venu Lagishetty Farzaneh Sedighian Farzaneh Sedighian Farzaneh Sedighian Shih-Lung Woo Benjamin W. Dreskin Benjamin W. Dreskin William Katzka William Katzka Candace Chang Candace Chang Yi Zhou Yi Zhou Nerea Arias-Jayo Nerea Arias-Jayo Julianne Yang Julianne Yang Aaron I. Ahdoot Aaron I. Ahdoot Jason Ye Jason Ye Zhaoping Li Zhaoping Li Joseph R. Pisegna Joseph R. Pisegna Joseph R. Pisegna Jonathan P. Jacobs Jonathan P. Jacobs Jonathan P. Jacobs Jonathan P. Jacobs |
| author_facet | Tien S. Dong Tien S. Dong Tien S. Dong Tien S. Dong Kayti Luu Kayti Luu Kayti Luu Kayti Luu Venu Lagishetty Venu Lagishetty Venu Lagishetty Venu Lagishetty Farzaneh Sedighian Farzaneh Sedighian Farzaneh Sedighian Shih-Lung Woo Benjamin W. Dreskin Benjamin W. Dreskin William Katzka William Katzka Candace Chang Candace Chang Yi Zhou Yi Zhou Nerea Arias-Jayo Nerea Arias-Jayo Julianne Yang Julianne Yang Aaron I. Ahdoot Aaron I. Ahdoot Jason Ye Jason Ye Zhaoping Li Zhaoping Li Joseph R. Pisegna Joseph R. Pisegna Joseph R. Pisegna Jonathan P. Jacobs Jonathan P. Jacobs Jonathan P. Jacobs Jonathan P. Jacobs |
| author_sort | Tien S. Dong |
| collection | DOAJ |
| description | Background: The microbiome has been shown in pre-clinical and epidemiological studies to be important in both the development and treatment of obesity and metabolic associated fatty liver disease (MAFLD). However, few studies have examined the role of the microbiome in the clinical response to calorie restriction. To explore this area, we performed a prospective study examining the association of the intestinal microbiome with weight loss and change in hepatic steatosis on a calorie-restricted diet.Methods: A prospective dietary intervention study of 80 overweight and obese participants was performed at the Greater West Los Angeles Veterans Affair Hospital. Patients were placed on a macronutrient standardized diet for 16 weeks, including 14 weeks of calorie restriction (500 calorie deficit). Body composition analysis by impedance, plasma lipid measurements, and ultrasound elastography to measure hepatic steatosis were performed at baseline and week 16. Intestinal microbiome composition was assessed using 16S rRNA gene sequencing. A per protocol analysis was performed on all subjects completing the trial (n = 46).Results: Study completers showed significant reduction in weight, body mass index, total cholesterol, low density lipoprotein, and triglyceride. Subjects who lost at least 5% of their body weight had significantly greater reduction in serum triglyceride and hepatic steatosis than those with <5% body weight loss. Enterococcus and Klebsiella were reduced at the end of the trial while Coprococcus and Collinsella were increased. There were also significant baseline microbiome differences between patients who had at least 5% weight loss as compared to those that did not. Lachnoclostridium was positively associated with hepatic steatosis and Actinomyces was positively associated with hepatic steatosis and weight. Baseline microbiome profiles were able to predict which patients lost at least 5% of their body weight with an AUROC of 0.80.Conclusion: Calorie restriction alters the intestinal microbiome and improves hepatic steatosis in those who experience significant weight loss. Baseline microbiome differences predict weight loss on a calorie–restricted diet and are associated with improvement in hepatic steatosis, suggesting a role of the gut microbiome in mediating the clinical response to calorie restriction. |
| first_indexed | 2024-12-18T00:50:47Z |
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| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-18T00:50:47Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-3c6cf76a8909426abcc92414e2a06d302022-12-21T21:26:41ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-07-01810.3389/fnut.2021.718661718661The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic SteatosisTien S. Dong0Tien S. Dong1Tien S. Dong2Tien S. Dong3Kayti Luu4Kayti Luu5Kayti Luu6Kayti Luu7Venu Lagishetty8Venu Lagishetty9Venu Lagishetty10Venu Lagishetty11Farzaneh Sedighian12Farzaneh Sedighian13Farzaneh Sedighian14Shih-Lung Woo15Benjamin W. Dreskin16Benjamin W. Dreskin17William Katzka18William Katzka19Candace Chang20Candace Chang21Yi Zhou22Yi Zhou23Nerea Arias-Jayo24Nerea Arias-Jayo25Julianne Yang26Julianne Yang27Aaron I. Ahdoot28Aaron I. Ahdoot29Jason Ye30Jason Ye31Zhaoping Li32Zhaoping Li33Joseph R. Pisegna34Joseph R. Pisegna35Joseph R. Pisegna36Jonathan P. Jacobs37Jonathan P. Jacobs38Jonathan P. Jacobs39Jonathan P. Jacobs40The Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesDivision of Gastroenterology, Hepatology, and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesDepartment of Medicine, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesDivision of Gastroenterology, Hepatology, and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesDepartment of Medicine, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesDivision of Gastroenterology, Hepatology, and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesDepartment of Medicine, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesDivision of Gastroenterology, Hepatology, and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesCenter for Human Nutrition, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesDivision of Gastroenterology, Hepatology, and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesDepartment of Medicine, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesDepartment of Medicine, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesCenter for Human Nutrition, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesDivision of Gastroenterology, Hepatology, and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesDepartment of Medicine, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesUCLA Microbiome Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United StatesDivision of Gastroenterology, Hepatology, and Parenteral Nutrition, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesDepartment of Medicine, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, CA, United StatesBackground: The microbiome has been shown in pre-clinical and epidemiological studies to be important in both the development and treatment of obesity and metabolic associated fatty liver disease (MAFLD). However, few studies have examined the role of the microbiome in the clinical response to calorie restriction. To explore this area, we performed a prospective study examining the association of the intestinal microbiome with weight loss and change in hepatic steatosis on a calorie-restricted diet.Methods: A prospective dietary intervention study of 80 overweight and obese participants was performed at the Greater West Los Angeles Veterans Affair Hospital. Patients were placed on a macronutrient standardized diet for 16 weeks, including 14 weeks of calorie restriction (500 calorie deficit). Body composition analysis by impedance, plasma lipid measurements, and ultrasound elastography to measure hepatic steatosis were performed at baseline and week 16. Intestinal microbiome composition was assessed using 16S rRNA gene sequencing. A per protocol analysis was performed on all subjects completing the trial (n = 46).Results: Study completers showed significant reduction in weight, body mass index, total cholesterol, low density lipoprotein, and triglyceride. Subjects who lost at least 5% of their body weight had significantly greater reduction in serum triglyceride and hepatic steatosis than those with <5% body weight loss. Enterococcus and Klebsiella were reduced at the end of the trial while Coprococcus and Collinsella were increased. There were also significant baseline microbiome differences between patients who had at least 5% weight loss as compared to those that did not. Lachnoclostridium was positively associated with hepatic steatosis and Actinomyces was positively associated with hepatic steatosis and weight. Baseline microbiome profiles were able to predict which patients lost at least 5% of their body weight with an AUROC of 0.80.Conclusion: Calorie restriction alters the intestinal microbiome and improves hepatic steatosis in those who experience significant weight loss. Baseline microbiome differences predict weight loss on a calorie–restricted diet and are associated with improvement in hepatic steatosis, suggesting a role of the gut microbiome in mediating the clinical response to calorie restriction.https://www.frontiersin.org/articles/10.3389/fnut.2021.718661/fullmetabolic syndromemetabolic associated fatty liver diseasemicrobiomeobesityultrasound elastographycontrolled attenuated parameter |
| spellingShingle | Tien S. Dong Tien S. Dong Tien S. Dong Tien S. Dong Kayti Luu Kayti Luu Kayti Luu Kayti Luu Venu Lagishetty Venu Lagishetty Venu Lagishetty Venu Lagishetty Farzaneh Sedighian Farzaneh Sedighian Farzaneh Sedighian Shih-Lung Woo Benjamin W. Dreskin Benjamin W. Dreskin William Katzka William Katzka Candace Chang Candace Chang Yi Zhou Yi Zhou Nerea Arias-Jayo Nerea Arias-Jayo Julianne Yang Julianne Yang Aaron I. Ahdoot Aaron I. Ahdoot Jason Ye Jason Ye Zhaoping Li Zhaoping Li Joseph R. Pisegna Joseph R. Pisegna Joseph R. Pisegna Jonathan P. Jacobs Jonathan P. Jacobs Jonathan P. Jacobs Jonathan P. Jacobs The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic Steatosis Frontiers in Nutrition metabolic syndrome metabolic associated fatty liver disease microbiome obesity ultrasound elastography controlled attenuated parameter |
| title | The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic Steatosis |
| title_full | The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic Steatosis |
| title_fullStr | The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic Steatosis |
| title_full_unstemmed | The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic Steatosis |
| title_short | The Intestinal Microbiome Predicts Weight Loss on a Calorie-Restricted Diet and Is Associated With Improved Hepatic Steatosis |
| title_sort | intestinal microbiome predicts weight loss on a calorie restricted diet and is associated with improved hepatic steatosis |
| topic | metabolic syndrome metabolic associated fatty liver disease microbiome obesity ultrasound elastography controlled attenuated parameter |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.718661/full |
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