Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat Model
(-)-Epigallocatechin 3-gallate (EGCG) is the main active green tea catechin and has a wide variety of benefits for health. Post-traumatic osteoarthritis (PTOA) occurs as a consequence of joint injuries that commonly happen in the young population. In this study, we investigated the effects of EGCG o...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/1/8 |
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| author | Hsuan-Ti Huang Tsung-Lin Cheng Cheng-Jung Ho Han Hsiang Huang Cheng-Chang Lu Shu-Chun Chuang Jhong-You Li Tien-Ching Lee Shih-Tse Chen Yi-Shan Lin Chih-Yao Lee Lin Kang Sung-Yen Lin Chung-Hwan Chen |
| author_facet | Hsuan-Ti Huang Tsung-Lin Cheng Cheng-Jung Ho Han Hsiang Huang Cheng-Chang Lu Shu-Chun Chuang Jhong-You Li Tien-Ching Lee Shih-Tse Chen Yi-Shan Lin Chih-Yao Lee Lin Kang Sung-Yen Lin Chung-Hwan Chen |
| author_sort | Hsuan-Ti Huang |
| collection | DOAJ |
| description | (-)-Epigallocatechin 3-gallate (EGCG) is the main active green tea catechin and has a wide variety of benefits for health. Post-traumatic osteoarthritis (PTOA) occurs as a consequence of joint injuries that commonly happen in the young population. In this study, we investigated the effects of EGCG on PTOA prevention by using the anterior cruciate ligament transection (ACLT)–OA model and further investigated the roles of autophagy in OA treatment. Our results showed that intra-articular injection of EGCG significantly improved the functional performances and decreased cartilage degradation. EGCG treatment attenuated the inflammation on synovial tissue and cartilage through less immunostained cyclooxygenase-2 and matrix metalloproteinase-13. We further noted EGCG may modulate the chondrocyte apoptosis by activation of the cytoprotective autophagy through reducing the expression of the mTOR and enhancing the expression of microtubule-associated protein light chain 3, beclin-1, and p62. In conclusion, intra-articular injection of EGCG after ACL injury inhibited the joint inflammation and cartilage degradation, thereby increasing joint function. EGCG treatment also reduced the chondrocyte apoptosis, possibly by activating autophagy. These findings suggested that EGCG may be a potential disease-modifying drug for preventing OA progression. |
| first_indexed | 2024-03-10T13:49:14Z |
| format | Article |
| id | doaj.art-3c6d668a2bf74a66b04d96f1df5343d2 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T13:49:14Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-3c6d668a2bf74a66b04d96f1df5343d22023-11-21T02:15:16ZengMDPI AGAntioxidants2076-39212020-12-01101810.3390/antiox10010008Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat ModelHsuan-Ti Huang0Tsung-Lin Cheng1Cheng-Jung Ho2Han Hsiang Huang3Cheng-Chang Lu4Shu-Chun Chuang5Jhong-You Li6Tien-Ching Lee7Shih-Tse Chen8Yi-Shan Lin9Chih-Yao Lee10Lin Kang11Sung-Yen Lin12Chung-Hwan Chen13Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanDepartment of Veterinary Medicine, National Chiayi University, Chiayi 60004, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanDepartment of Psychiatry, National Taiwan University Hospital Hsin-Chu Branch, Hsin Chu 30059, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanDepartment of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70101, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, Taiwan(-)-Epigallocatechin 3-gallate (EGCG) is the main active green tea catechin and has a wide variety of benefits for health. Post-traumatic osteoarthritis (PTOA) occurs as a consequence of joint injuries that commonly happen in the young population. In this study, we investigated the effects of EGCG on PTOA prevention by using the anterior cruciate ligament transection (ACLT)–OA model and further investigated the roles of autophagy in OA treatment. Our results showed that intra-articular injection of EGCG significantly improved the functional performances and decreased cartilage degradation. EGCG treatment attenuated the inflammation on synovial tissue and cartilage through less immunostained cyclooxygenase-2 and matrix metalloproteinase-13. We further noted EGCG may modulate the chondrocyte apoptosis by activation of the cytoprotective autophagy through reducing the expression of the mTOR and enhancing the expression of microtubule-associated protein light chain 3, beclin-1, and p62. In conclusion, intra-articular injection of EGCG after ACL injury inhibited the joint inflammation and cartilage degradation, thereby increasing joint function. EGCG treatment also reduced the chondrocyte apoptosis, possibly by activating autophagy. These findings suggested that EGCG may be a potential disease-modifying drug for preventing OA progression.https://www.mdpi.com/2076-3921/10/1/8(-)-epigallocatechin 3-gallate (EGCG)apoptosisautophagycartilagemTORpost-traumatic osteoarthritis |
| spellingShingle | Hsuan-Ti Huang Tsung-Lin Cheng Cheng-Jung Ho Han Hsiang Huang Cheng-Chang Lu Shu-Chun Chuang Jhong-You Li Tien-Ching Lee Shih-Tse Chen Yi-Shan Lin Chih-Yao Lee Lin Kang Sung-Yen Lin Chung-Hwan Chen Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat Model Antioxidants (-)-epigallocatechin 3-gallate (EGCG) apoptosis autophagy cartilage mTOR post-traumatic osteoarthritis |
| title | Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat Model |
| title_full | Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat Model |
| title_fullStr | Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat Model |
| title_full_unstemmed | Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat Model |
| title_short | Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat Model |
| title_sort | intra articular injection of epigallocatechin 3 gallate to attenuate articular cartilage degeneration by enhancing autophagy in a post traumatic osteoarthritis rat model |
| topic | (-)-epigallocatechin 3-gallate (EGCG) apoptosis autophagy cartilage mTOR post-traumatic osteoarthritis |
| url | https://www.mdpi.com/2076-3921/10/1/8 |
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