Microbial communities associated with epilithic algal matrix with different morphological characters in Luhuitou fringing reef
The microbiota is an important component of the epilithic algal matrix (EAM) and plays a central role in the biogeochemical cycling of important nutrients in coral reef ecosystems. Insufficient studies on EAM microbiota diversity have led to a limited understanding of the ecological functions of EAM...
| Main Authors: | , , , , , |
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| Format: | Article |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.993305/full |
| _version_ | 1797948715327029248 |
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| author | Tiancheng Zhou Tiancheng Zhou Tiancheng Zhou Simin Hu Simin Hu Simin Hu Nan Jia Nan Jia Nan Jia Chen Zhang Chen Zhang Chen Zhang Hui Huang Hui Huang Hui Huang Hui Huang Hui Huang Sheng Liu Sheng Liu Sheng Liu |
| author_facet | Tiancheng Zhou Tiancheng Zhou Tiancheng Zhou Simin Hu Simin Hu Simin Hu Nan Jia Nan Jia Nan Jia Chen Zhang Chen Zhang Chen Zhang Hui Huang Hui Huang Hui Huang Hui Huang Hui Huang Sheng Liu Sheng Liu Sheng Liu |
| author_sort | Tiancheng Zhou |
| collection | DOAJ |
| description | The microbiota is an important component of the epilithic algal matrix (EAM) and plays a central role in the biogeochemical cycling of important nutrients in coral reef ecosystems. Insufficient studies on EAM microbiota diversity have led to a limited understanding of the ecological functions of EAMs in different states. To explore the microbial community of EAMs in the Luhuitou fringing reef in Sanya, China, which has undergone the incessant expansion and domination of algae over the past several decades, investigations were conducted in the reef’s intertidal zone. Five types of substrate habitats (dead branching coral, dead massive coral, dead flat coral, granite block, and concrete block) were selected, and their microbial communities were analyzed by high-throughput sequencing of EAM holobionts using the 16S rDNA V4 region. Proteobacteria was the most abundant group, accounting for more than 70% of reads of the microbial composition across all sites, followed by Cyanobacteria (15.89%) and Bacteroidetes (5.93%), respectively. Cluster analysis divided all microbial communities into three groups, namely short, medium, and long EAMs. Algal length was the most important morphological factor impacting the differences in the composition of the EAM microbiota. The three EAM groups had 52 common OTUs and 78.52% common sequences, among which the most abundant were Vibrio spp. and Photobacterium spp. The three types of EAM also had unique OTUs. The short EAMs had 238 unique OTUs and 48.61% unique sequences, mainly in the genera Shewanella and Cyanobacterium. The medium EAMs contained 130 unique OTUs and 4.36% unique sequences, mainly in the genera Pseudomonas and Bacillus. The long EAMs only had 27 unique OTUs and 4.13% unique sequences, mainly in the genus Marinobacter. Compared with short EAM, medium and long EAM had a lower proportion of autotrophic bacteria and higher proportion of potential pathogenic bacteria. It is suggested that EAMs with different phenotypes have different microbial compositions, and the ecological function of the EAM microbiota changes from autotrophic to pathogenic with an increase in algal length. As EAMs have expanded on coastal coral reefs worldwide, it is essential to comprehensively explore the community structure and ecological role of their microbial communities. |
| first_indexed | 2024-04-10T21:47:43Z |
| format | Article |
| id | doaj.art-3c732e6829b44a06a608774c14be927f |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-10T21:47:43Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-3c732e6829b44a06a608774c14be927f2023-01-18T13:50:02ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-10-01910.3389/fmars.2022.993305993305Microbial communities associated with epilithic algal matrix with different morphological characters in Luhuitou fringing reefTiancheng Zhou0Tiancheng Zhou1Tiancheng Zhou2Simin Hu3Simin Hu4Simin Hu5Nan Jia6Nan Jia7Nan Jia8Chen Zhang9Chen Zhang10Chen Zhang11Hui Huang12Hui Huang13Hui Huang14Hui Huang15Hui Huang16Sheng Liu17Sheng Liu18Sheng Liu19Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaCollege of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaSanya Joint Laboratory of Marine Science Research, Key Laboratory of Tropical Marine Biotechnology of Hainan Province, Sanya Institute of Oceanology, South China Sea Institute of Oceanology, Sanya, ChinaKey Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaCollege of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaCollege of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaSanya Joint Laboratory of Marine Science Research, Key Laboratory of Tropical Marine Biotechnology of Hainan Province, Sanya Institute of Oceanology, South China Sea Institute of Oceanology, Sanya, ChinaTropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, Sanya, ChinaSanya National Marine Ecosystem Research Station, Chinese Academy of Sciences, Sanya, ChinaKey Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaSanya Joint Laboratory of Marine Science Research, Key Laboratory of Tropical Marine Biotechnology of Hainan Province, Sanya Institute of Oceanology, South China Sea Institute of Oceanology, Sanya, ChinaThe microbiota is an important component of the epilithic algal matrix (EAM) and plays a central role in the biogeochemical cycling of important nutrients in coral reef ecosystems. Insufficient studies on EAM microbiota diversity have led to a limited understanding of the ecological functions of EAMs in different states. To explore the microbial community of EAMs in the Luhuitou fringing reef in Sanya, China, which has undergone the incessant expansion and domination of algae over the past several decades, investigations were conducted in the reef’s intertidal zone. Five types of substrate habitats (dead branching coral, dead massive coral, dead flat coral, granite block, and concrete block) were selected, and their microbial communities were analyzed by high-throughput sequencing of EAM holobionts using the 16S rDNA V4 region. Proteobacteria was the most abundant group, accounting for more than 70% of reads of the microbial composition across all sites, followed by Cyanobacteria (15.89%) and Bacteroidetes (5.93%), respectively. Cluster analysis divided all microbial communities into three groups, namely short, medium, and long EAMs. Algal length was the most important morphological factor impacting the differences in the composition of the EAM microbiota. The three EAM groups had 52 common OTUs and 78.52% common sequences, among which the most abundant were Vibrio spp. and Photobacterium spp. The three types of EAM also had unique OTUs. The short EAMs had 238 unique OTUs and 48.61% unique sequences, mainly in the genera Shewanella and Cyanobacterium. The medium EAMs contained 130 unique OTUs and 4.36% unique sequences, mainly in the genera Pseudomonas and Bacillus. The long EAMs only had 27 unique OTUs and 4.13% unique sequences, mainly in the genus Marinobacter. Compared with short EAM, medium and long EAM had a lower proportion of autotrophic bacteria and higher proportion of potential pathogenic bacteria. It is suggested that EAMs with different phenotypes have different microbial compositions, and the ecological function of the EAM microbiota changes from autotrophic to pathogenic with an increase in algal length. As EAMs have expanded on coastal coral reefs worldwide, it is essential to comprehensively explore the community structure and ecological role of their microbial communities.https://www.frontiersin.org/articles/10.3389/fmars.2022.993305/fullmicrobiotacommunity structureepilithic algal matrixcoral reefmorphological difference |
| spellingShingle | Tiancheng Zhou Tiancheng Zhou Tiancheng Zhou Simin Hu Simin Hu Simin Hu Nan Jia Nan Jia Nan Jia Chen Zhang Chen Zhang Chen Zhang Hui Huang Hui Huang Hui Huang Hui Huang Hui Huang Sheng Liu Sheng Liu Sheng Liu Microbial communities associated with epilithic algal matrix with different morphological characters in Luhuitou fringing reef Frontiers in Marine Science microbiota community structure epilithic algal matrix coral reef morphological difference |
| title | Microbial communities associated with epilithic algal matrix with different morphological characters in Luhuitou fringing reef |
| title_full | Microbial communities associated with epilithic algal matrix with different morphological characters in Luhuitou fringing reef |
| title_fullStr | Microbial communities associated with epilithic algal matrix with different morphological characters in Luhuitou fringing reef |
| title_full_unstemmed | Microbial communities associated with epilithic algal matrix with different morphological characters in Luhuitou fringing reef |
| title_short | Microbial communities associated with epilithic algal matrix with different morphological characters in Luhuitou fringing reef |
| title_sort | microbial communities associated with epilithic algal matrix with different morphological characters in luhuitou fringing reef |
| topic | microbiota community structure epilithic algal matrix coral reef morphological difference |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.993305/full |
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