Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in <i>Dictyostelium discoideum</i>
<i>Dictyostelium</i> cells undergo a semi-closed mitosis, during which the nuclear envelope (NE) persists; however, free diffusion between the cytoplasm and the nucleus takes place. To permit the formation of the mitotic spindle, the nuclear envelope must be permeabilized in order to all...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/3/407 |
| _version_ | 1797488502536929280 |
|---|---|
| author | Kristina Mitic Marianne Grafe Petros Batsios Irene Meyer |
| author_facet | Kristina Mitic Marianne Grafe Petros Batsios Irene Meyer |
| author_sort | Kristina Mitic |
| collection | DOAJ |
| description | <i>Dictyostelium</i> cells undergo a semi-closed mitosis, during which the nuclear envelope (NE) persists; however, free diffusion between the cytoplasm and the nucleus takes place. To permit the formation of the mitotic spindle, the nuclear envelope must be permeabilized in order to allow diffusion of tubulin dimers and spindle assembly factors into the nucleus. In Aspergillus, free diffusion of proteins between the cytoplasm and the nucleus is achieved by a partial disassembly of the nuclear pore complexes (NPCs) prior to spindle assembly. In order to determine whether this is also the case in <i>Dictyostelium</i>, we analysed components of the NPC by immunofluorescence microscopy and live cell imaging and studied their behaviour during interphase and mitosis. We observed that the NPCs are absent from the contact area of the nucleoli and that some nucleoporins also localize to the centrosome and the spindle poles. In addition, we could show that, during mitosis, the central FG protein NUP62, two inner ring components and Gle1 depart from the NPCs, while all other tested NUPs remained at the NE. This leads to the conclusion that indeed a partial disassembly of the NPCs takes place, which contributes to permeabilisation of the NE during semi-closed mitosis. |
| first_indexed | 2024-03-10T00:04:08Z |
| format | Article |
| id | doaj.art-3c737f27acb246fe968fdcd53df6187f |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:04:08Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3c737f27acb246fe968fdcd53df6187f2023-11-23T16:11:21ZengMDPI AGCells2073-44092022-01-0111340710.3390/cells11030407Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in <i>Dictyostelium discoideum</i>Kristina Mitic0Marianne Grafe1Petros Batsios2Irene Meyer3Department of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, GermanyDepartment of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, GermanyDepartment of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, GermanyDepartment of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, Germany<i>Dictyostelium</i> cells undergo a semi-closed mitosis, during which the nuclear envelope (NE) persists; however, free diffusion between the cytoplasm and the nucleus takes place. To permit the formation of the mitotic spindle, the nuclear envelope must be permeabilized in order to allow diffusion of tubulin dimers and spindle assembly factors into the nucleus. In Aspergillus, free diffusion of proteins between the cytoplasm and the nucleus is achieved by a partial disassembly of the nuclear pore complexes (NPCs) prior to spindle assembly. In order to determine whether this is also the case in <i>Dictyostelium</i>, we analysed components of the NPC by immunofluorescence microscopy and live cell imaging and studied their behaviour during interphase and mitosis. We observed that the NPCs are absent from the contact area of the nucleoli and that some nucleoporins also localize to the centrosome and the spindle poles. In addition, we could show that, during mitosis, the central FG protein NUP62, two inner ring components and Gle1 depart from the NPCs, while all other tested NUPs remained at the NE. This leads to the conclusion that indeed a partial disassembly of the NPCs takes place, which contributes to permeabilisation of the NE during semi-closed mitosis.https://www.mdpi.com/2073-4409/11/3/407nuclear pore complexnucleoporinssemi-closed mitosiscentrosome<i>Dictyostelium</i> |
| spellingShingle | Kristina Mitic Marianne Grafe Petros Batsios Irene Meyer Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in <i>Dictyostelium discoideum</i> Cells nuclear pore complex nucleoporins semi-closed mitosis centrosome <i>Dictyostelium</i> |
| title | Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in <i>Dictyostelium discoideum</i> |
| title_full | Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in <i>Dictyostelium discoideum</i> |
| title_fullStr | Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in <i>Dictyostelium discoideum</i> |
| title_full_unstemmed | Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in <i>Dictyostelium discoideum</i> |
| title_short | Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in <i>Dictyostelium discoideum</i> |
| title_sort | partial disassembly of the nuclear pore complex proteins during semi closed mitosis in i dictyostelium discoideum i |
| topic | nuclear pore complex nucleoporins semi-closed mitosis centrosome <i>Dictyostelium</i> |
| url | https://www.mdpi.com/2073-4409/11/3/407 |
| work_keys_str_mv | AT kristinamitic partialdisassemblyofthenuclearporecomplexproteinsduringsemiclosedmitosisinidictyosteliumdiscoideumi AT mariannegrafe partialdisassemblyofthenuclearporecomplexproteinsduringsemiclosedmitosisinidictyosteliumdiscoideumi AT petrosbatsios partialdisassemblyofthenuclearporecomplexproteinsduringsemiclosedmitosisinidictyosteliumdiscoideumi AT irenemeyer partialdisassemblyofthenuclearporecomplexproteinsduringsemiclosedmitosisinidictyosteliumdiscoideumi |