A role for MCP-1/CCR2 in interstitial lung disease in children
<p>Abstract</p> <p>Background</p> <p>Interstitial lung diseases (ILD) are chronic inflammatory disorders leading to pulmonary fibrosis. Monocyte chemotactic protein 1 (MCP-1) promotes collagen synthesis and deletion of the MCP-1 receptor CCR2 protects from pulmonary fib...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-08-01
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| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | http://respiratory-research.com/content/6/1/93 |
| _version_ | 1818021045614936064 |
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| author | Reinhardt Dietrich Prell Christine Zissel Gernot Nicolai Thomas Griese Matthias Hartl Dominik Schendel Dolores J Krauss-Etschmann Susanne |
| author_facet | Reinhardt Dietrich Prell Christine Zissel Gernot Nicolai Thomas Griese Matthias Hartl Dominik Schendel Dolores J Krauss-Etschmann Susanne |
| author_sort | Reinhardt Dietrich |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Interstitial lung diseases (ILD) are chronic inflammatory disorders leading to pulmonary fibrosis. Monocyte chemotactic protein 1 (MCP-1) promotes collagen synthesis and deletion of the MCP-1 receptor CCR2 protects from pulmonary fibrosis in ILD mouse models. We hypothesized that pulmonary MCP-1 and CCR2<sup>+ </sup>T cells accumulate in pediatric ILD and are related to disease severity.</p> <p>Methods</p> <p>Bronchoalveolar lavage fluid was obtained from 25 children with ILD and 10 healthy children. Levels of pulmonary MCP-1 and Th1/Th2-associated cytokines were quantified at the protein and the mRNA levels. Pulmonary CCR2<sup>+</sup>, CCR4<sup>+</sup>, CCR3<sup>+</sup>, CCR5<sup>+ </sup>and CXCR3<sup>+ </sup>T cells were quantified by flow-cytometry.</p> <p>Results</p> <p>CCR2<sup>+ </sup>T cells and MCP-1 levels were significantly elevated in children with ILD and correlated with forced vital capacity, total lung capacity and ILD disease severity scores. Children with lung fibrosis had significantly higher MCP-1 levels and CCR2<sup>+ </sup>T cells in bronchoalveolar lavage fluid compared to non-fibrotic children.</p> <p>Conclusion</p> <p>The results indicate that pulmonary CCR2<sup>+ </sup>T cells and MCP-1 contribute to the pathogenesis of pediatric ILD and might provide a novel target for therapeutic strategies.</p> |
| first_indexed | 2024-04-14T08:13:38Z |
| format | Article |
| id | doaj.art-3c74eecf73284bc19ac4ba97460fd3f6 |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-04-14T08:13:38Z |
| publishDate | 2005-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-3c74eecf73284bc19ac4ba97460fd3f62022-12-22T02:04:28ZengBMCRespiratory Research1465-99212005-08-01619310.1186/1465-9921-6-93A role for MCP-1/CCR2 in interstitial lung disease in childrenReinhardt DietrichPrell ChristineZissel GernotNicolai ThomasGriese MatthiasHartl DominikSchendel Dolores JKrauss-Etschmann Susanne<p>Abstract</p> <p>Background</p> <p>Interstitial lung diseases (ILD) are chronic inflammatory disorders leading to pulmonary fibrosis. Monocyte chemotactic protein 1 (MCP-1) promotes collagen synthesis and deletion of the MCP-1 receptor CCR2 protects from pulmonary fibrosis in ILD mouse models. We hypothesized that pulmonary MCP-1 and CCR2<sup>+ </sup>T cells accumulate in pediatric ILD and are related to disease severity.</p> <p>Methods</p> <p>Bronchoalveolar lavage fluid was obtained from 25 children with ILD and 10 healthy children. Levels of pulmonary MCP-1 and Th1/Th2-associated cytokines were quantified at the protein and the mRNA levels. Pulmonary CCR2<sup>+</sup>, CCR4<sup>+</sup>, CCR3<sup>+</sup>, CCR5<sup>+ </sup>and CXCR3<sup>+ </sup>T cells were quantified by flow-cytometry.</p> <p>Results</p> <p>CCR2<sup>+ </sup>T cells and MCP-1 levels were significantly elevated in children with ILD and correlated with forced vital capacity, total lung capacity and ILD disease severity scores. Children with lung fibrosis had significantly higher MCP-1 levels and CCR2<sup>+ </sup>T cells in bronchoalveolar lavage fluid compared to non-fibrotic children.</p> <p>Conclusion</p> <p>The results indicate that pulmonary CCR2<sup>+ </sup>T cells and MCP-1 contribute to the pathogenesis of pediatric ILD and might provide a novel target for therapeutic strategies.</p>http://respiratory-research.com/content/6/1/93ChemokinesMCP-1CCR2Bronchoalveolar LavageChildrenInterstitial Lung Diseases |
| spellingShingle | Reinhardt Dietrich Prell Christine Zissel Gernot Nicolai Thomas Griese Matthias Hartl Dominik Schendel Dolores J Krauss-Etschmann Susanne A role for MCP-1/CCR2 in interstitial lung disease in children Respiratory Research Chemokines MCP-1 CCR2 Bronchoalveolar Lavage Children Interstitial Lung Diseases |
| title | A role for MCP-1/CCR2 in interstitial lung disease in children |
| title_full | A role for MCP-1/CCR2 in interstitial lung disease in children |
| title_fullStr | A role for MCP-1/CCR2 in interstitial lung disease in children |
| title_full_unstemmed | A role for MCP-1/CCR2 in interstitial lung disease in children |
| title_short | A role for MCP-1/CCR2 in interstitial lung disease in children |
| title_sort | role for mcp 1 ccr2 in interstitial lung disease in children |
| topic | Chemokines MCP-1 CCR2 Bronchoalveolar Lavage Children Interstitial Lung Diseases |
| url | http://respiratory-research.com/content/6/1/93 |
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