Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species
Orthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-endemic Tatenale virus, is sparse. In this study, 531 animals from five rodent species were collected from two...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/4/454 |
| _version_ | 1797570303150260224 |
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| author | Joseph G. Chappell Theocharis Tsoleridis Okechukwu Onianwa Gabby Drake Ian Ashpole Phillipa Dobbs William Edema Frederick Kumi-Ansah Malcolm Bennett Rachael E. Tarlinton Jonathan K. Ball C. Patrick McClure |
| author_facet | Joseph G. Chappell Theocharis Tsoleridis Okechukwu Onianwa Gabby Drake Ian Ashpole Phillipa Dobbs William Edema Frederick Kumi-Ansah Malcolm Bennett Rachael E. Tarlinton Jonathan K. Ball C. Patrick McClure |
| author_sort | Joseph G. Chappell |
| collection | DOAJ |
| description | Orthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-endemic Tatenale virus, is sparse. In this study, 531 animals from five rodent species were collected from two locations in northern and central England and screened using a degenerate, pan- orthohantavirus RT-PCR assay. Tatenale virus was detected in a single field vole (<i>Microtus agrestis</i>) from central England and twelve field voles from northern England. Unbiased high-throughput sequencing of the central English strain resulted in the recovery of the complete coding sequence of a novel strain of Tatenale virus, whilst PCR-primer walking of the northern English strain recovered almost complete coding sequence of a previously identified strain. These findings represented the detection of a third lineage of Tatenale virus in the United Kingdom and extended the known geographic distribution of these viruses from northern to central England. Furthermore, the recovery of the complete coding sequence revealed that Tatenale virus was sufficiently related to the recently identified Traemersee virus, to meet the accepted criteria for classification as a single species of orthohantavirus. |
| first_indexed | 2024-03-10T20:23:01Z |
| format | Article |
| id | doaj.art-3c768eceaff8466385bad3da648db33b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T20:23:01Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-3c768eceaff8466385bad3da648db33b2023-11-19T21:58:51ZengMDPI AGViruses1999-49152020-04-0112445410.3390/v12040454Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel SpeciesJoseph G. Chappell0Theocharis Tsoleridis1Okechukwu Onianwa2Gabby Drake3Ian Ashpole4Phillipa Dobbs5William Edema6Frederick Kumi-Ansah7Malcolm Bennett8Rachael E. Tarlinton9Jonathan K. Ball10C. Patrick McClure11School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKChester Zoo, Chester, Cheshire CH2 1EU, UKChester Zoo, Chester, Cheshire CH2 1EU, UKTwycross Zoo, Atherstone, Warwickshire CV9 3PX, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Veterinary Science, University of Nottingham, Sutton Bonnington, Loughborough LE12 5RD, UKSchool of Veterinary Science, University of Nottingham, Sutton Bonnington, Loughborough LE12 5RD, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKOrthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-endemic Tatenale virus, is sparse. In this study, 531 animals from five rodent species were collected from two locations in northern and central England and screened using a degenerate, pan- orthohantavirus RT-PCR assay. Tatenale virus was detected in a single field vole (<i>Microtus agrestis</i>) from central England and twelve field voles from northern England. Unbiased high-throughput sequencing of the central English strain resulted in the recovery of the complete coding sequence of a novel strain of Tatenale virus, whilst PCR-primer walking of the northern English strain recovered almost complete coding sequence of a previously identified strain. These findings represented the detection of a third lineage of Tatenale virus in the United Kingdom and extended the known geographic distribution of these viruses from northern to central England. Furthermore, the recovery of the complete coding sequence revealed that Tatenale virus was sufficiently related to the recently identified Traemersee virus, to meet the accepted criteria for classification as a single species of orthohantavirus.https://www.mdpi.com/1999-4915/12/4/454<i>Orthohantavirus</i>hantavirushigh-throughput sequencingvirus discoveryfield voleUnited Kingdom |
| spellingShingle | Joseph G. Chappell Theocharis Tsoleridis Okechukwu Onianwa Gabby Drake Ian Ashpole Phillipa Dobbs William Edema Frederick Kumi-Ansah Malcolm Bennett Rachael E. Tarlinton Jonathan K. Ball C. Patrick McClure Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species Viruses <i>Orthohantavirus</i> hantavirus high-throughput sequencing virus discovery field vole United Kingdom |
| title | Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species |
| title_full | Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species |
| title_fullStr | Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species |
| title_full_unstemmed | Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species |
| title_short | Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species |
| title_sort | retrieval of the complete coding sequence of the uk endemic tatenale orthohantavirus reveals extensive strain variation and supports its classification as a novel species |
| topic | <i>Orthohantavirus</i> hantavirus high-throughput sequencing virus discovery field vole United Kingdom |
| url | https://www.mdpi.com/1999-4915/12/4/454 |
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