A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis
Apicomplexan parasites of the genus <i>Babesia</i> cause babesiosis in humans and animals. The microscopic examination of stained blood smears, detection of serum antibodies by immunoassays, and PCR-based identification of parasite nucleic acid in blood are common laboratory methods for...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/10/6/377 |
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| author | Jyotsna S. Shah Olivia Mark Eddie Caoili Akhila Poruri Richard I. Horowitz Alan D. Ashbaugh Ranjan Ramasamy |
| author_facet | Jyotsna S. Shah Olivia Mark Eddie Caoili Akhila Poruri Richard I. Horowitz Alan D. Ashbaugh Ranjan Ramasamy |
| author_sort | Jyotsna S. Shah |
| collection | DOAJ |
| description | Apicomplexan parasites of the genus <i>Babesia</i> cause babesiosis in humans and animals. The microscopic examination of stained blood smears, detection of serum antibodies by immunoassays, and PCR-based identification of parasite nucleic acid in blood are common laboratory methods for diagnosing babesiosis. The present study evaluated a commercially available <i>Babesia</i> genus-specific fluorescence in situ hybridization (FISH) test for detecting <i>Babesia</i> parasites in blood smears. The FISH test detected <i>Babesia duncani</i> and <i>Babesia microti</i>, two common species that cause human infections in the USA, and other <i>Babesia</i> species of human and veterinary importance in less than two hours. The <i>Babesia</i> genus-specific FISH test supplements other existing laboratory methods for diagnosing babesiosis and may be particularly useful in resource-limited laboratories. |
| first_indexed | 2024-03-10T19:19:41Z |
| format | Article |
| id | doaj.art-3c76e8e7e0e64cd5ba935def4e27ad25 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T19:19:41Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-3c76e8e7e0e64cd5ba935def4e27ad252023-11-20T03:03:31ZengMDPI AGDiagnostics2075-44182020-06-0110637710.3390/diagnostics10060377A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing BabesiosisJyotsna S. Shah0Olivia Mark1Eddie Caoili2Akhila Poruri3Richard I. Horowitz4Alan D. Ashbaugh5Ranjan Ramasamy6ID-FISH Technology Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USAIGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USAIGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USAIGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USAHudson Valley Healing Arts Center, New York, NY 12538, USACollege of Medicine, University of Cincinnati, Cincinnati, OH 45221, USAID-FISH Technology Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USAApicomplexan parasites of the genus <i>Babesia</i> cause babesiosis in humans and animals. The microscopic examination of stained blood smears, detection of serum antibodies by immunoassays, and PCR-based identification of parasite nucleic acid in blood are common laboratory methods for diagnosing babesiosis. The present study evaluated a commercially available <i>Babesia</i> genus-specific fluorescence in situ hybridization (FISH) test for detecting <i>Babesia</i> parasites in blood smears. The FISH test detected <i>Babesia duncani</i> and <i>Babesia microti</i>, two common species that cause human infections in the USA, and other <i>Babesia</i> species of human and veterinary importance in less than two hours. The <i>Babesia</i> genus-specific FISH test supplements other existing laboratory methods for diagnosing babesiosis and may be particularly useful in resource-limited laboratories.https://www.mdpi.com/2075-4418/10/6/377<i>Babesia</i>babesiosisFISHfluorescence in situ hybridizationlaboratory diagnosis of babesiosis |
| spellingShingle | Jyotsna S. Shah Olivia Mark Eddie Caoili Akhila Poruri Richard I. Horowitz Alan D. Ashbaugh Ranjan Ramasamy A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis Diagnostics <i>Babesia</i> babesiosis FISH fluorescence in situ hybridization laboratory diagnosis of babesiosis |
| title | A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis |
| title_full | A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis |
| title_fullStr | A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis |
| title_full_unstemmed | A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis |
| title_short | A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis |
| title_sort | fluorescence in situ hybridization fish test for diagnosing babesiosis |
| topic | <i>Babesia</i> babesiosis FISH fluorescence in situ hybridization laboratory diagnosis of babesiosis |
| url | https://www.mdpi.com/2075-4418/10/6/377 |
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