BMP-13 Emerges as a Potential Inhibitor of Bone Formation
<p>Bone morphogenetic protein-13 (BMP-13) plays an important role in skeletal development. In the light of a recent report that mutations in the BMP-13 gene are associated with spine vertebral fusion in Klippel-Feil syndrome, we hypothesized that BMP-13 signaling is crucial for regulating embr...
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2009-01-01
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| Series: | International Journal of Biological Sciences |
| Online Access: | http://www.biolsci.org/v05p0192.htm |
| _version_ | 1828542598231359488 |
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| author | Bojiang Shen, Divya Bhargav, Aiqun Wei, Lisa A Williams, Helen Tao, David D F Ma, Ashish D Diwan |
| author_facet | Bojiang Shen, Divya Bhargav, Aiqun Wei, Lisa A Williams, Helen Tao, David D F Ma, Ashish D Diwan |
| author_sort | Bojiang Shen, Divya Bhargav, Aiqun Wei, Lisa A Williams, Helen Tao, David D F Ma, Ashish D Diwan |
| collection | DOAJ |
| description | <p>Bone morphogenetic protein-13 (BMP-13) plays an important role in skeletal development. In the light of a recent report that mutations in the BMP-13 gene are associated with spine vertebral fusion in Klippel-Feil syndrome, we hypothesized that BMP-13 signaling is crucial for regulating embryonic endochondral ossification. In this study, we found that BMP-13 inhibited the osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells (BM MSCs) in vitro. The endogenous BMP-13 gene expression in MSCs was examined under expansion conditions. The MSCs were then induced to differentiate into osteoblasts in osteo-inductive medium containing exogenous BMP-13. Gene expression was analysed by real-time PCR. Alkaline phosphatase (ALP) expression and activity, proteoglycan (PG) synthesis and matrix mineralization were assessed by cytological staining or ALP assay. Results showed that endogenous BMP-13 mRNA expression was higher than BMP-2 or -7 during MSC growth. BMP-13 supplementation strongly inhibited matrix mineralization and ALP activity of osteogenic differentiated MSCs, yet increased PG synthesis under the same conditions. In conclusion, BMP-13 inhibited osteogenic differentiation of MSCs, implying that functional mutations or deficiency of BMP-13 may allow excess bone formation. Our finding provides an insight into the molecular mechanisms and the therapeutic potential of BMP-13 in restricting pathological bone formation.</p> |
| first_indexed | 2024-12-12T01:59:00Z |
| format | Article |
| id | doaj.art-3c7b180cf51745ec9a3867da30c70fc3 |
| institution | Directory Open Access Journal |
| issn | 1449-2288 |
| language | English |
| last_indexed | 2024-12-12T01:59:00Z |
| publishDate | 2009-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Biological Sciences |
| spelling | doaj.art-3c7b180cf51745ec9a3867da30c70fc32022-12-22T00:42:14ZengIvyspring International PublisherInternational Journal of Biological Sciences1449-22882009-01-0152192200BMP-13 Emerges as a Potential Inhibitor of Bone FormationBojiang Shen, Divya Bhargav, Aiqun Wei, Lisa A Williams, Helen Tao, David D F Ma, Ashish D Diwan<p>Bone morphogenetic protein-13 (BMP-13) plays an important role in skeletal development. In the light of a recent report that mutations in the BMP-13 gene are associated with spine vertebral fusion in Klippel-Feil syndrome, we hypothesized that BMP-13 signaling is crucial for regulating embryonic endochondral ossification. In this study, we found that BMP-13 inhibited the osteogenic differentiation of human bone marrow multipotent mesenchymal stromal cells (BM MSCs) in vitro. The endogenous BMP-13 gene expression in MSCs was examined under expansion conditions. The MSCs were then induced to differentiate into osteoblasts in osteo-inductive medium containing exogenous BMP-13. Gene expression was analysed by real-time PCR. Alkaline phosphatase (ALP) expression and activity, proteoglycan (PG) synthesis and matrix mineralization were assessed by cytological staining or ALP assay. Results showed that endogenous BMP-13 mRNA expression was higher than BMP-2 or -7 during MSC growth. BMP-13 supplementation strongly inhibited matrix mineralization and ALP activity of osteogenic differentiated MSCs, yet increased PG synthesis under the same conditions. In conclusion, BMP-13 inhibited osteogenic differentiation of MSCs, implying that functional mutations or deficiency of BMP-13 may allow excess bone formation. Our finding provides an insight into the molecular mechanisms and the therapeutic potential of BMP-13 in restricting pathological bone formation.</p>http://www.biolsci.org/v05p0192.htm |
| spellingShingle | Bojiang Shen, Divya Bhargav, Aiqun Wei, Lisa A Williams, Helen Tao, David D F Ma, Ashish D Diwan BMP-13 Emerges as a Potential Inhibitor of Bone Formation International Journal of Biological Sciences |
| title | BMP-13 Emerges as a Potential Inhibitor of Bone Formation |
| title_full | BMP-13 Emerges as a Potential Inhibitor of Bone Formation |
| title_fullStr | BMP-13 Emerges as a Potential Inhibitor of Bone Formation |
| title_full_unstemmed | BMP-13 Emerges as a Potential Inhibitor of Bone Formation |
| title_short | BMP-13 Emerges as a Potential Inhibitor of Bone Formation |
| title_sort | bmp 13 emerges as a potential inhibitor of bone formation |
| url | http://www.biolsci.org/v05p0192.htm |
| work_keys_str_mv | AT bojiangshendivyabhargavaiqunweilisaawilliamshelentaodaviddfmaashishddiwan bmp13emergesasapotentialinhibitorofboneformation |