Genome editing in East African cichlids and tilapias: state-of-the-art and future directions
African cichlid fishes of the Cichlidae family are a group of teleosts important for aquaculture and research. A thriving research community is particularly interested in the cichlid radiations of the East African Great Lakes. One key goal is to pinpoint genetic variation underlying phenotypic diver...
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| Format: | Article |
| Language: | English |
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The Royal Society
2023-11-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.230257 |
| _version_ | 1797360991920455680 |
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| author | Bethan Clark Muktai Kuwalekar Bettina Fischer Joost Woltering Jakob Biran Scott Juntti Claudius F. Kratochwil M. Emília Santos Miguel Vasconcelos Almeida |
| author_facet | Bethan Clark Muktai Kuwalekar Bettina Fischer Joost Woltering Jakob Biran Scott Juntti Claudius F. Kratochwil M. Emília Santos Miguel Vasconcelos Almeida |
| author_sort | Bethan Clark |
| collection | DOAJ |
| description | African cichlid fishes of the Cichlidae family are a group of teleosts important for aquaculture and research. A thriving research community is particularly interested in the cichlid radiations of the East African Great Lakes. One key goal is to pinpoint genetic variation underlying phenotypic diversification, but the lack of genetic tools has precluded thorough dissection of the genetic basis of relevant traits in cichlids. Genome editing technologies are well established in teleost models like zebrafish and medaka. However, this is not the case for emerging model organisms, such as East African cichlids, where these technologies remain inaccessible to most laboratories, due in part to limited exchange of knowledge and expertise. The Cichlid Science 2022 meeting (Cambridge, UK) hosted for the first time a Genome Editing Workshop, where the community discussed recent advances in genome editing, with an emphasis on CRISPR/Cas9 technologies. Based on the workshop findings and discussions, in this review we define the state-of-the-art of cichlid genome editing, share resources and protocols, and propose new possible avenues to further expand the cichlid genome editing toolkit. |
| first_indexed | 2024-03-08T15:47:33Z |
| format | Article |
| id | doaj.art-3c7b752ad8304ccbbd1a491628e9686e |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-03-08T15:47:33Z |
| publishDate | 2023-11-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-3c7b752ad8304ccbbd1a491628e9686e2024-01-09T09:30:51ZengThe Royal SocietyOpen Biology2046-24412023-11-01131110.1098/rsob.230257Genome editing in East African cichlids and tilapias: state-of-the-art and future directionsBethan Clark0Muktai Kuwalekar1Bettina Fischer2Joost Woltering3Jakob Biran4Scott Juntti5Claudius F. Kratochwil6M. Emília Santos7Miguel Vasconcelos Almeida8Department of Zoology, University of Cambridge, Cambridge, UKHelsinki Institute of Life Science (HiLIFE), University of Helsinki, Helsinki, Uusimaa 00014, FinlandDepartment of Genetics, University of Cambridge, Cambridge, UKZoology and Evolutionary Biology, Department of Biology, University of Konstanz, Konstanz, Baden-Württemberg 78457, GermanyDepartment of Poultry and Aquaculture, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Rishon Lezion, IsraelDepartment of Biology, University of Maryland, College Park, MD, USAHelsinki Institute of Life Science (HiLIFE), University of Helsinki, Helsinki, Uusimaa 00014, FinlandDepartment of Zoology, University of Cambridge, Cambridge, UKDepartment of Biochemistry, University of Cambridge, Cambridge, UKAfrican cichlid fishes of the Cichlidae family are a group of teleosts important for aquaculture and research. A thriving research community is particularly interested in the cichlid radiations of the East African Great Lakes. One key goal is to pinpoint genetic variation underlying phenotypic diversification, but the lack of genetic tools has precluded thorough dissection of the genetic basis of relevant traits in cichlids. Genome editing technologies are well established in teleost models like zebrafish and medaka. However, this is not the case for emerging model organisms, such as East African cichlids, where these technologies remain inaccessible to most laboratories, due in part to limited exchange of knowledge and expertise. The Cichlid Science 2022 meeting (Cambridge, UK) hosted for the first time a Genome Editing Workshop, where the community discussed recent advances in genome editing, with an emphasis on CRISPR/Cas9 technologies. Based on the workshop findings and discussions, in this review we define the state-of-the-art of cichlid genome editing, share resources and protocols, and propose new possible avenues to further expand the cichlid genome editing toolkit.https://royalsocietypublishing.org/doi/10.1098/rsob.230257CRISPR/Cas9genome editingtilapiaemerging model organismsEast African cichlids |
| spellingShingle | Bethan Clark Muktai Kuwalekar Bettina Fischer Joost Woltering Jakob Biran Scott Juntti Claudius F. Kratochwil M. Emília Santos Miguel Vasconcelos Almeida Genome editing in East African cichlids and tilapias: state-of-the-art and future directions Open Biology CRISPR/Cas9 genome editing tilapia emerging model organisms East African cichlids |
| title | Genome editing in East African cichlids and tilapias: state-of-the-art and future directions |
| title_full | Genome editing in East African cichlids and tilapias: state-of-the-art and future directions |
| title_fullStr | Genome editing in East African cichlids and tilapias: state-of-the-art and future directions |
| title_full_unstemmed | Genome editing in East African cichlids and tilapias: state-of-the-art and future directions |
| title_short | Genome editing in East African cichlids and tilapias: state-of-the-art and future directions |
| title_sort | genome editing in east african cichlids and tilapias state of the art and future directions |
| topic | CRISPR/Cas9 genome editing tilapia emerging model organisms East African cichlids |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.230257 |
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