Screening for the Active Anti-Inflammatory and Antioxidant Polyphenols of <i>Gaultheria procumbens</i> and Their Application for Standardisation: From Identification through Cellular Studies to Quantitative Determination
Aerial parts, leaves, and stems of <i>Gaultheria procumbens</i> are polyphenol-rich herbal medicines with anti-inflammatory and antioxidant effects. The present study focused on identifying active markers of the <i>G. procumbens</i> extracts in an integrated approach combinin...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11532 |
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| author | Monika Anna Olszewska Aleksandra Owczarek Anna Magiera Sebastian Granica Piotr Michel |
| author_facet | Monika Anna Olszewska Aleksandra Owczarek Anna Magiera Sebastian Granica Piotr Michel |
| author_sort | Monika Anna Olszewska |
| collection | DOAJ |
| description | Aerial parts, leaves, and stems of <i>Gaultheria procumbens</i> are polyphenol-rich herbal medicines with anti-inflammatory and antioxidant effects. The present study focused on identifying active markers of the <i>G. procumbens</i> extracts in an integrated approach combining phytochemical and biological capacity tests. The target compounds, representing all classes of <i>Gaultheria</i> polyphenols, were pre-selected by LC-ESI-PDA-MS/MS. For unambiguous identification, the key analytes, including a rare procyanidin trimer (cinnamtannin B-1), miquelianin potassium salt, and two new natural products: quercetin and kaempferol 3-<i>O</i>-<i>β</i>-<span style="font-variant: small-caps;">d</span>-xylopyranosyl-(1→2)-<i>β</i>-<span style="font-variant: small-caps;">d</span>-glucuronopyranosides, were isolated by preparative HPLC and investigated by spectroscopy (HR-ESI-MS, UV-vis, CD, 1D- and 2D-NMR), thiolysis, flame photometry, optical rotation experiments, and absolute configuration studies. The significant contribution of the pre-selected compounds to the biological effects of the extracts was confirmed in vitro: the analytes significantly and in a dose-dependent manner down-regulated the pro-oxidant and pro-inflammatory functions of human neutrophils ex vivo (inhibited the release of reactive oxygen species, IL-1β, TNF-α, and neutrophils elastase, ELA-2), inhibited two key pro-inflammatory enzymes (cyclooxygenase, COX-2, and hyaluronidase), and most of them, except gaultherin, exerted potent direct antioxidant activity (ferric reducing antioxidant power and superoxide anion scavenging capacity). Moreover, cellular safety was confirmed for all compounds by flow cytometry. Eventually, as these mechanisms have been connected to the health benefits of <i>G. procumbens</i>, 11 polyphenols were accepted as active markers, and a simple, accurate, reproducible, and fully validated RP-HPLC-PDA method for standardisation of the target extracts was proposed. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:01:30Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3c7c4169014943c2969ef4cdc82457c62023-11-22T20:53:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122211153210.3390/ijms222111532Screening for the Active Anti-Inflammatory and Antioxidant Polyphenols of <i>Gaultheria procumbens</i> and Their Application for Standardisation: From Identification through Cellular Studies to Quantitative DeterminationMonika Anna Olszewska0Aleksandra Owczarek1Anna Magiera2Sebastian Granica3Piotr Michel4Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, Muszynskiego 1 St., 90-151 Lodz, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, Muszynskiego 1 St., 90-151 Lodz, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, Muszynskiego 1 St., 90-151 Lodz, PolandMicrobiota Lab, Centre for Preclinical Studies, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, 1 Banacha St., 02-097 Warsaw, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, Muszynskiego 1 St., 90-151 Lodz, PolandAerial parts, leaves, and stems of <i>Gaultheria procumbens</i> are polyphenol-rich herbal medicines with anti-inflammatory and antioxidant effects. The present study focused on identifying active markers of the <i>G. procumbens</i> extracts in an integrated approach combining phytochemical and biological capacity tests. The target compounds, representing all classes of <i>Gaultheria</i> polyphenols, were pre-selected by LC-ESI-PDA-MS/MS. For unambiguous identification, the key analytes, including a rare procyanidin trimer (cinnamtannin B-1), miquelianin potassium salt, and two new natural products: quercetin and kaempferol 3-<i>O</i>-<i>β</i>-<span style="font-variant: small-caps;">d</span>-xylopyranosyl-(1→2)-<i>β</i>-<span style="font-variant: small-caps;">d</span>-glucuronopyranosides, were isolated by preparative HPLC and investigated by spectroscopy (HR-ESI-MS, UV-vis, CD, 1D- and 2D-NMR), thiolysis, flame photometry, optical rotation experiments, and absolute configuration studies. The significant contribution of the pre-selected compounds to the biological effects of the extracts was confirmed in vitro: the analytes significantly and in a dose-dependent manner down-regulated the pro-oxidant and pro-inflammatory functions of human neutrophils ex vivo (inhibited the release of reactive oxygen species, IL-1β, TNF-α, and neutrophils elastase, ELA-2), inhibited two key pro-inflammatory enzymes (cyclooxygenase, COX-2, and hyaluronidase), and most of them, except gaultherin, exerted potent direct antioxidant activity (ferric reducing antioxidant power and superoxide anion scavenging capacity). Moreover, cellular safety was confirmed for all compounds by flow cytometry. Eventually, as these mechanisms have been connected to the health benefits of <i>G. procumbens</i>, 11 polyphenols were accepted as active markers, and a simple, accurate, reproducible, and fully validated RP-HPLC-PDA method for standardisation of the target extracts was proposed.https://www.mdpi.com/1422-0067/22/21/11532<i>Gaultheria procumbens</i>anti-inflammatory activityantioxidant activityisolationstructure elucidationquercetin 3-<i>O</i>-<i>β</i>-<span style="font-variant: small-caps">d</span>-xylopyranosyl-(1→2)-<i>β</i>-<span style="font-variant: small-caps">d</span>-glucuronopyranoside |
| spellingShingle | Monika Anna Olszewska Aleksandra Owczarek Anna Magiera Sebastian Granica Piotr Michel Screening for the Active Anti-Inflammatory and Antioxidant Polyphenols of <i>Gaultheria procumbens</i> and Their Application for Standardisation: From Identification through Cellular Studies to Quantitative Determination International Journal of Molecular Sciences <i>Gaultheria procumbens</i> anti-inflammatory activity antioxidant activity isolation structure elucidation quercetin 3-<i>O</i>-<i>β</i>-<span style="font-variant: small-caps">d</span>-xylopyranosyl-(1→2)-<i>β</i>-<span style="font-variant: small-caps">d</span>-glucuronopyranoside |
| title | Screening for the Active Anti-Inflammatory and Antioxidant Polyphenols of <i>Gaultheria procumbens</i> and Their Application for Standardisation: From Identification through Cellular Studies to Quantitative Determination |
| title_full | Screening for the Active Anti-Inflammatory and Antioxidant Polyphenols of <i>Gaultheria procumbens</i> and Their Application for Standardisation: From Identification through Cellular Studies to Quantitative Determination |
| title_fullStr | Screening for the Active Anti-Inflammatory and Antioxidant Polyphenols of <i>Gaultheria procumbens</i> and Their Application for Standardisation: From Identification through Cellular Studies to Quantitative Determination |
| title_full_unstemmed | Screening for the Active Anti-Inflammatory and Antioxidant Polyphenols of <i>Gaultheria procumbens</i> and Their Application for Standardisation: From Identification through Cellular Studies to Quantitative Determination |
| title_short | Screening for the Active Anti-Inflammatory and Antioxidant Polyphenols of <i>Gaultheria procumbens</i> and Their Application for Standardisation: From Identification through Cellular Studies to Quantitative Determination |
| title_sort | screening for the active anti inflammatory and antioxidant polyphenols of i gaultheria procumbens i and their application for standardisation from identification through cellular studies to quantitative determination |
| topic | <i>Gaultheria procumbens</i> anti-inflammatory activity antioxidant activity isolation structure elucidation quercetin 3-<i>O</i>-<i>β</i>-<span style="font-variant: small-caps">d</span>-xylopyranosyl-(1→2)-<i>β</i>-<span style="font-variant: small-caps">d</span>-glucuronopyranoside |
| url | https://www.mdpi.com/1422-0067/22/21/11532 |
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