AtHB7/12 Regulate Root Growth in Response to Aluminum Stress
Aluminum (Al) stress is a major limiting factor for plant growth and crop production in acid soils. At present, only a few transcription factors involved in the regulation of Al resistance have been characterized. Here, we used reversed genetic approach through phenotype analysis of overexpressors a...
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/11/4080 |
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| author | Yang Liu Jiameng Xu Siyi Guo Xianzheng Yuan Shan Zhao Huiyu Tian Shaojun Dai Xiangpei Kong Zhaojun Ding |
| author_facet | Yang Liu Jiameng Xu Siyi Guo Xianzheng Yuan Shan Zhao Huiyu Tian Shaojun Dai Xiangpei Kong Zhaojun Ding |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Aluminum (Al) stress is a major limiting factor for plant growth and crop production in acid soils. At present, only a few transcription factors involved in the regulation of Al resistance have been characterized. Here, we used reversed genetic approach through phenotype analysis of overexpressors and mutants to demonstrate that AtHB7 and AtHB12, two HD-Zip I transcription factors, participate in Al resistance. In response to Al stress, <i>AtHB7</i> and <i>AtHB12</i> displayed different dynamic expression patterns. Although both AtHB7 and AtHB12 positively regulate root growth in the absence of Al stress, our results showed that AtHB7 antagonizes with AtHB12 to control root growth in response to Al stress. The <i>athb7/12</i> double mutant displayed a wild-type phenotype under Al stress. Consistently, our physiological analysis showed that AtHB7 and AtHB12 oppositely regulate the capacity of cell wall to bind Al. Yeast two hybrid assays showed that AtHB7 and AtHB12 could form homo-dimers and hetero-dimers in vitro, suggesting the interaction between AtHB7 and AtHB12 in the regulation of root growth. The conclusion was that AtHB7 and AtHB12 oppositely regulate Al resistance by affecting Al accumulation in root cell wall. |
| first_indexed | 2024-03-10T19:18:44Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:18:44Z |
| publishDate | 2020-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3c7ccb685ab442c9816587008d68c8ef2023-11-20T03:07:39ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012111408010.3390/ijms21114080AtHB7/12 Regulate Root Growth in Response to Aluminum StressYang Liu0Jiameng Xu1Siyi Guo2Xianzheng Yuan3Shan Zhao4Huiyu Tian5Shaojun Dai6Xiangpei Kong7Zhaojun Ding8The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, College of Life Sciences, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, College of Life Sciences, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Stress Biology, School of Life Science, Henan University, JinMing Avenue, Henan University, Kaifeng 475004, ChinaShandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, ChinaShandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, College of Life Sciences, Shandong University, Qingdao 266237, ChinaDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai 200234, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, College of Life Sciences, Shandong University, Qingdao 266237, ChinaThe Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, College of Life Sciences, Shandong University, Qingdao 266237, ChinaAluminum (Al) stress is a major limiting factor for plant growth and crop production in acid soils. At present, only a few transcription factors involved in the regulation of Al resistance have been characterized. Here, we used reversed genetic approach through phenotype analysis of overexpressors and mutants to demonstrate that AtHB7 and AtHB12, two HD-Zip I transcription factors, participate in Al resistance. In response to Al stress, <i>AtHB7</i> and <i>AtHB12</i> displayed different dynamic expression patterns. Although both AtHB7 and AtHB12 positively regulate root growth in the absence of Al stress, our results showed that AtHB7 antagonizes with AtHB12 to control root growth in response to Al stress. The <i>athb7/12</i> double mutant displayed a wild-type phenotype under Al stress. Consistently, our physiological analysis showed that AtHB7 and AtHB12 oppositely regulate the capacity of cell wall to bind Al. Yeast two hybrid assays showed that AtHB7 and AtHB12 could form homo-dimers and hetero-dimers in vitro, suggesting the interaction between AtHB7 and AtHB12 in the regulation of root growth. The conclusion was that AtHB7 and AtHB12 oppositely regulate Al resistance by affecting Al accumulation in root cell wall.https://www.mdpi.com/1422-0067/21/11/4080rootaluminum stressAtHB7/12yeast two hybridHD-Zip I transcription factors |
| spellingShingle | Yang Liu Jiameng Xu Siyi Guo Xianzheng Yuan Shan Zhao Huiyu Tian Shaojun Dai Xiangpei Kong Zhaojun Ding AtHB7/12 Regulate Root Growth in Response to Aluminum Stress International Journal of Molecular Sciences root aluminum stress AtHB7/12 yeast two hybrid HD-Zip I transcription factors |
| title | AtHB7/12 Regulate Root Growth in Response to Aluminum Stress |
| title_full | AtHB7/12 Regulate Root Growth in Response to Aluminum Stress |
| title_fullStr | AtHB7/12 Regulate Root Growth in Response to Aluminum Stress |
| title_full_unstemmed | AtHB7/12 Regulate Root Growth in Response to Aluminum Stress |
| title_short | AtHB7/12 Regulate Root Growth in Response to Aluminum Stress |
| title_sort | athb7 12 regulate root growth in response to aluminum stress |
| topic | root aluminum stress AtHB7/12 yeast two hybrid HD-Zip I transcription factors |
| url | https://www.mdpi.com/1422-0067/21/11/4080 |
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