Metagenomic Analyses of the Soybean Root Mycobiome and Microbiome Reveal Signatures of the Healthy and Diseased Plants Affected by Taproot Decline
Invading pathogens interact with plant-associated microbial communities, which can be altered under the pressure of pathogen infection. Limited information exists on plant–microbe interactions occurring during natural outbreaks in agricultural fields. Taproot decline (TRD) of soybean is an emerging...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/5/856 |
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| author | Sorina C. Popescu Maria Tomaso-Peterson Teresa Wilkerson Aline Bronzato-Badial Uyen Wesser George V. Popescu |
| author_facet | Sorina C. Popescu Maria Tomaso-Peterson Teresa Wilkerson Aline Bronzato-Badial Uyen Wesser George V. Popescu |
| author_sort | Sorina C. Popescu |
| collection | DOAJ |
| description | Invading pathogens interact with plant-associated microbial communities, which can be altered under the pressure of pathogen infection. Limited information exists on plant–microbe interactions occurring during natural outbreaks in agricultural fields. Taproot decline (TRD) of soybean is an emerging disease caused by <i>Xylaria necrophora.</i> TRD disease occurrence and yield loss associated with TRD are outstanding issues in soybean production. We applied nuclear ribosomal DNA Internal Transcribed Spacers and 16S rRNA gene taxonomic marker sequencing to define the composition of the fungal and bacterial communities associated with healthy and diseased soybean roots collected from the Mississippi Delta. The plant compartment was a significant factor regulating taxonomic diversity, followed by the disease status of the plant. TRD impacted the root endophytes, causing imbalances; at the intermediate and advanced stages of TRD, <i>X. necrophora</i> decreased mycobiome diversity, whereas it increased microbiome richness. Networks of significant co-occurrence and co-exclusion relationships revealed direct and indirect associations among taxa and identified hubs with potential roles in assembling healthy and TRD-affected soybean biomes. These studies advance the understanding of host–microbe interactions in TRD and the part of biomes in plant health and disease. |
| first_indexed | 2024-03-10T03:22:55Z |
| format | Article |
| id | doaj.art-3c7cd21d2d884042a3be2d3e004d1db3 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:22:55Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3c7cd21d2d884042a3be2d3e004d1db32023-11-23T12:13:58ZengMDPI AGMicroorganisms2076-26072022-04-0110585610.3390/microorganisms10050856Metagenomic Analyses of the Soybean Root Mycobiome and Microbiome Reveal Signatures of the Healthy and Diseased Plants Affected by Taproot DeclineSorina C. Popescu0Maria Tomaso-Peterson1Teresa Wilkerson2Aline Bronzato-Badial3Uyen Wesser4George V. Popescu5Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USADepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USADepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USADepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USADepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USAInstitute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USAInvading pathogens interact with plant-associated microbial communities, which can be altered under the pressure of pathogen infection. Limited information exists on plant–microbe interactions occurring during natural outbreaks in agricultural fields. Taproot decline (TRD) of soybean is an emerging disease caused by <i>Xylaria necrophora.</i> TRD disease occurrence and yield loss associated with TRD are outstanding issues in soybean production. We applied nuclear ribosomal DNA Internal Transcribed Spacers and 16S rRNA gene taxonomic marker sequencing to define the composition of the fungal and bacterial communities associated with healthy and diseased soybean roots collected from the Mississippi Delta. The plant compartment was a significant factor regulating taxonomic diversity, followed by the disease status of the plant. TRD impacted the root endophytes, causing imbalances; at the intermediate and advanced stages of TRD, <i>X. necrophora</i> decreased mycobiome diversity, whereas it increased microbiome richness. Networks of significant co-occurrence and co-exclusion relationships revealed direct and indirect associations among taxa and identified hubs with potential roles in assembling healthy and TRD-affected soybean biomes. These studies advance the understanding of host–microbe interactions in TRD and the part of biomes in plant health and disease.https://www.mdpi.com/2076-2607/10/5/856soybean rootmicrobiomemycobiometaproot decline<i>Xylaria necrophora</i> |
| spellingShingle | Sorina C. Popescu Maria Tomaso-Peterson Teresa Wilkerson Aline Bronzato-Badial Uyen Wesser George V. Popescu Metagenomic Analyses of the Soybean Root Mycobiome and Microbiome Reveal Signatures of the Healthy and Diseased Plants Affected by Taproot Decline Microorganisms soybean root microbiome mycobiome taproot decline <i>Xylaria necrophora</i> |
| title | Metagenomic Analyses of the Soybean Root Mycobiome and Microbiome Reveal Signatures of the Healthy and Diseased Plants Affected by Taproot Decline |
| title_full | Metagenomic Analyses of the Soybean Root Mycobiome and Microbiome Reveal Signatures of the Healthy and Diseased Plants Affected by Taproot Decline |
| title_fullStr | Metagenomic Analyses of the Soybean Root Mycobiome and Microbiome Reveal Signatures of the Healthy and Diseased Plants Affected by Taproot Decline |
| title_full_unstemmed | Metagenomic Analyses of the Soybean Root Mycobiome and Microbiome Reveal Signatures of the Healthy and Diseased Plants Affected by Taproot Decline |
| title_short | Metagenomic Analyses of the Soybean Root Mycobiome and Microbiome Reveal Signatures of the Healthy and Diseased Plants Affected by Taproot Decline |
| title_sort | metagenomic analyses of the soybean root mycobiome and microbiome reveal signatures of the healthy and diseased plants affected by taproot decline |
| topic | soybean root microbiome mycobiome taproot decline <i>Xylaria necrophora</i> |
| url | https://www.mdpi.com/2076-2607/10/5/856 |
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