The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae
Sterol 14α-demethylase catalyzes lanosterol hydroxylation, which is one of the key reactions in the biosynthetic pathway of sterols. There is only one sterol 14α-demethylases gene named Erg11 in Saccharomyces cerevisiae genome. In this study, three sterol 14α-demethylases genes named AoErg11A, AoErg...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1009746/full |
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| author | Qi Jin Ganghua Li Kunhai Qin Yitong Shang Huanhuan Yan Hongliang Liu Bin Zeng Zhihong Hu |
| author_facet | Qi Jin Ganghua Li Kunhai Qin Yitong Shang Huanhuan Yan Hongliang Liu Bin Zeng Zhihong Hu |
| author_sort | Qi Jin |
| collection | DOAJ |
| description | Sterol 14α-demethylase catalyzes lanosterol hydroxylation, which is one of the key reactions in the biosynthetic pathway of sterols. There is only one sterol 14α-demethylases gene named Erg11 in Saccharomyces cerevisiae genome. In this study, three sterol 14α-demethylases genes named AoErg11A, AoErg11B and AoErg11C were identified in Aspergillus oryzae genome through bioinformatics analysis. The function of these three genes were studied by yeast complementation, and the expression pattern/subcellular localization of these genes/proteins were detected. The results showed that the three AoErg11s were expressed differently at different growth times and under different abiotic stresses. All of the three proteins were located in endoplasmic reticulum. The AoErg11s could not restore the temperature-sensitive phenotype of S. cerevisiae erg11 mutant. Overexpression of the three AoErg11s affected both growth and sporulation, which may be due to the effect of AoErg11s on ergosterol content. Therefore, this study revealed the functions of three AoErg11s and their effects on the growth and ergosterol biosynthesis of A. oryzae, which may contribute to the further understanding of the ergosterol biosynthesis and regulation mechanism in this important filamentous fungus, A. oryzae. |
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| language | English |
| last_indexed | 2024-04-10T20:55:23Z |
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| series | Frontiers in Genetics |
| spelling | doaj.art-3c7ec8682de24163be962636f73e5ca32023-01-23T05:14:46ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-01-011410.3389/fgene.2023.10097461009746The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzaeQi Jin0Ganghua Li1Kunhai Qin2Yitong Shang3Huanhuan Yan4Hongliang Liu5Bin Zeng6Zhihong Hu7Jiangxi Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, ChinaHubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi, ChinaJiangxi Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, ChinaJiangxi Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, ChinaJiangxi Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, ChinaJiangxi Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen, ChinaJiangxi Key Laboratory of Bioprocess Engineering, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, ChinaSterol 14α-demethylase catalyzes lanosterol hydroxylation, which is one of the key reactions in the biosynthetic pathway of sterols. There is only one sterol 14α-demethylases gene named Erg11 in Saccharomyces cerevisiae genome. In this study, three sterol 14α-demethylases genes named AoErg11A, AoErg11B and AoErg11C were identified in Aspergillus oryzae genome through bioinformatics analysis. The function of these three genes were studied by yeast complementation, and the expression pattern/subcellular localization of these genes/proteins were detected. The results showed that the three AoErg11s were expressed differently at different growth times and under different abiotic stresses. All of the three proteins were located in endoplasmic reticulum. The AoErg11s could not restore the temperature-sensitive phenotype of S. cerevisiae erg11 mutant. Overexpression of the three AoErg11s affected both growth and sporulation, which may be due to the effect of AoErg11s on ergosterol content. Therefore, this study revealed the functions of three AoErg11s and their effects on the growth and ergosterol biosynthesis of A. oryzae, which may contribute to the further understanding of the ergosterol biosynthesis and regulation mechanism in this important filamentous fungus, A. oryzae.https://www.frontiersin.org/articles/10.3389/fgene.2023.1009746/fullAspergillus oryzaesterol 14α-demethylaseergosterolsubcellular localizationfunction |
| spellingShingle | Qi Jin Ganghua Li Kunhai Qin Yitong Shang Huanhuan Yan Hongliang Liu Bin Zeng Zhihong Hu The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae Frontiers in Genetics Aspergillus oryzae sterol 14α-demethylase ergosterol subcellular localization function |
| title | The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae |
| title_full | The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae |
| title_fullStr | The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae |
| title_full_unstemmed | The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae |
| title_short | The expression pattern, subcellular localization and function of three sterol 14α-demethylases in Aspergillus oryzae |
| title_sort | expression pattern subcellular localization and function of three sterol 14α demethylases in aspergillus oryzae |
| topic | Aspergillus oryzae sterol 14α-demethylase ergosterol subcellular localization function |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1009746/full |
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