Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s
The type VI secretion system (T6SS) is a new secretion system that is widely distributed among Gram-negative bacteria. The core component hemolysin-coregulated protein (Hcp) can be used as both its structural protein and secretory protein or chaperone protein. Studies on Hcp are important to elucida...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2020.1854538 |
| _version_ | 1819096355605315584 |
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| author | Ping Wang Jun-Fang Dong Ren-Qing Li Lei Li Qing-Hua Zou |
| author_facet | Ping Wang Jun-Fang Dong Ren-Qing Li Lei Li Qing-Hua Zou |
| author_sort | Ping Wang |
| collection | DOAJ |
| description | The type VI secretion system (T6SS) is a new secretion system that is widely distributed among Gram-negative bacteria. The core component hemolysin-coregulated protein (Hcp) can be used as both its structural protein and secretory protein or chaperone protein. Studies on Hcp are important to elucidate the overall virulence mechanism of T6SS. Salmonella typhimurium is an important foodborne pathogen. There are three copies of hcp genes identified in S. Typhimurium 14028s. This study aimed to characterize the functions of the three Hcp family proteins and to elucidate the interactions among them. The hcp gene deletion mutants were constructed by λ Red-based recombination system. Effects of hcp mutation on the pathogenicity of 14028s were studied by bacterial competition assays, Dictyostelium discoideum assays and mouse model. The three Hcp family proteins were found to play different roles. Hcp1 can affect the transcription of rpoS and type 2 flagellar gene and influence the motility of 14028s. It is also involved in the intracellular survival of 14028s in Dictyostelium discoideum; Hcp2 is involved in the early proliferative capacity of 14028s in mice and can prevent its excessive proliferation; Hcp3 did not show direct functions in these assays. Hcp1 can interact with Hcp2 and Hcp3. Deletion of one hcp gene can result in a transcription level variation in the other two hcp genes. Our findings elucidated the functions of the three Hcp family proteins in S.Typhimurium and illustrated that there are interactions between different Hcp proteins. This study will be helpful to fully understand how T6SS actions in an organism. |
| first_indexed | 2024-12-21T23:57:53Z |
| format | Article |
| id | doaj.art-3c7feb24d5d649f992b63912f5bf494a |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-12-21T23:57:53Z |
| publishDate | 2020-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-3c7feb24d5d649f992b63912f5bf494a2022-12-21T18:45:47ZengTaylor & Francis GroupVirulence2150-55942150-56082020-12-011111716172610.1080/21505594.2020.18545381854538Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028sPing Wang0Jun-Fang Dong1Ren-Qing Li2Lei Li3Qing-Hua Zou4Peking University Health Science CenterPeking University Health Science CenterInstitute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive MedicinePeking University Health Science CenterPeking University Health Science CenterThe type VI secretion system (T6SS) is a new secretion system that is widely distributed among Gram-negative bacteria. The core component hemolysin-coregulated protein (Hcp) can be used as both its structural protein and secretory protein or chaperone protein. Studies on Hcp are important to elucidate the overall virulence mechanism of T6SS. Salmonella typhimurium is an important foodborne pathogen. There are three copies of hcp genes identified in S. Typhimurium 14028s. This study aimed to characterize the functions of the three Hcp family proteins and to elucidate the interactions among them. The hcp gene deletion mutants were constructed by λ Red-based recombination system. Effects of hcp mutation on the pathogenicity of 14028s were studied by bacterial competition assays, Dictyostelium discoideum assays and mouse model. The three Hcp family proteins were found to play different roles. Hcp1 can affect the transcription of rpoS and type 2 flagellar gene and influence the motility of 14028s. It is also involved in the intracellular survival of 14028s in Dictyostelium discoideum; Hcp2 is involved in the early proliferative capacity of 14028s in mice and can prevent its excessive proliferation; Hcp3 did not show direct functions in these assays. Hcp1 can interact with Hcp2 and Hcp3. Deletion of one hcp gene can result in a transcription level variation in the other two hcp genes. Our findings elucidated the functions of the three Hcp family proteins in S.Typhimurium and illustrated that there are interactions between different Hcp proteins. This study will be helpful to fully understand how T6SS actions in an organism.http://dx.doi.org/10.1080/21505594.2020.1854538salmonella typhimuriumtype vi secretion system(t6ss)hemolysin-coregulatory protein (hcp)virulenceflagella |
| spellingShingle | Ping Wang Jun-Fang Dong Ren-Qing Li Lei Li Qing-Hua Zou Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s Virulence salmonella typhimurium type vi secretion system(t6ss) hemolysin-coregulatory protein (hcp) virulence flagella |
| title | Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s |
| title_full | Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s |
| title_fullStr | Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s |
| title_full_unstemmed | Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s |
| title_short | Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s |
| title_sort | roles of the hcp family proteins in the pathogenicity of salmonella typhimurium 14028s |
| topic | salmonella typhimurium type vi secretion system(t6ss) hemolysin-coregulatory protein (hcp) virulence flagella |
| url | http://dx.doi.org/10.1080/21505594.2020.1854538 |
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