Purification of Cry1Ab protein by multi-cycles ion-exchange chromatography and its peptide mass mapping(多次循环离子交换色谱纯化Cry1Ab蛋白及其肽质量谱图的分析)
采用Q-Sepharose FF离子交换柱对初步纯化的Cry1Ab杀虫蛋白样品进行了多次循环精细纯化，洗脱模式为离子强度台阶梯度，流动相为Tris-HCl缓冲液加NaCl，盐浓度从0.1 mol •L-1变化到1.0 mol • L-1.结果表明，Cry1Ab蛋白在NaCl浓度为0.4 mol • L-1时从柱上被洗脱，并与杂蛋白很好地分离.色谱分离机制符合顶替色谱机制.经3次循环分离后可获得高纯度的Cry1Ab蛋白，用SDS-PAGE分析了蛋白的纯度及表观分子量，电泳胶上分离的蛋白进行原位酶切后，用基质辅助激光解析-飞行时间质谱对纯化蛋白进行肽质量谱的分析，Mascot数据库搜索结果确证了该...
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2007-09-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2007.34.5.529-532 |
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| author | LUANMing-ming(栾明明) WUJian-min(邬建敏) YEQing-fu(叶庆富) |
| author_facet | LUANMing-ming(栾明明) WUJian-min(邬建敏) YEQing-fu(叶庆富) |
| author_sort | LUANMing-ming(栾明明) |
| collection | DOAJ |
| description | 采用Q-Sepharose FF离子交换柱对初步纯化的Cry1Ab杀虫蛋白样品进行了多次循环精细纯化，洗脱模式为离子强度台阶梯度，流动相为Tris-HCl缓冲液加NaCl，盐浓度从0.1 mol •L-1变化到1.0 mol • L-1.结果表明，Cry1Ab蛋白在NaCl浓度为0.4 mol • L-1时从柱上被洗脱，并与杂蛋白很好地分离.色谱分离机制符合顶替色谱机制.经3次循环分离后可获得高纯度的Cry1Ab蛋白，用SDS-PAGE分析了蛋白的纯度及表观分子量，电泳胶上分离的蛋白进行原位酶切后，用基质辅助激光解析-飞行时间质谱对纯化蛋白进行肽质量谱的分析，Mascot数据库搜索结果确证了该纯化蛋白酶解肽段80%符合已知的Cry1Ab蛋白特征舦段. |
| first_indexed | 2024-04-24T17:00:21Z |
| format | Article |
| id | doaj.art-3c7ffa161e804bb48d66df1936f29700 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:00:21Z |
| publishDate | 2007-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-3c7ffa161e804bb48d66df1936f297002024-03-29T01:58:25ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972007-09-01345529532zjup/1008-9497.2007.34.5.529-532Purification of Cry1Ab protein by multi-cycles ion-exchange chromatography and its peptide mass mapping(多次循环离子交换色谱纯化Cry1Ab蛋白及其肽质量谱图的分析)LUANMing-ming(栾明明)0WUJian-min(邬建敏)1YEQing-fu(叶庆富)2 1.Department of Chemistry, Zhejiang University, Hangzhou 310027, China( 1.浙江大学化学系，浙江 杭州 310027) 1.Department of Chemistry, Zhejiang University, Hangzhou 310027, China( 1.浙江大学化学系，浙江 杭州 310027) 2.Institute of Nucleat-Agricultural Science, Zhejiang University, Hangzhou 310029, China( 2.浙江大学核农所，浙江 杭州 310029)采用Q-Sepharose FF离子交换柱对初步纯化的Cry1Ab杀虫蛋白样品进行了多次循环精细纯化，洗脱模式为离子强度台阶梯度，流动相为Tris-HCl缓冲液加NaCl，盐浓度从0.1 mol •L-1变化到1.0 mol • L-1.结果表明，Cry1Ab蛋白在NaCl浓度为0.4 mol • L-1时从柱上被洗脱，并与杂蛋白很好地分离.色谱分离机制符合顶替色谱机制.经3次循环分离后可获得高纯度的Cry1Ab蛋白，用SDS-PAGE分析了蛋白的纯度及表观分子量，电泳胶上分离的蛋白进行原位酶切后，用基质辅助激光解析-飞行时间质谱对纯化蛋白进行肽质量谱的分析，Mascot数据库搜索结果确证了该纯化蛋白酶解肽段80%符合已知的Cry1Ab蛋白特征舦段.https://doi.org/zjup/1008-9497.2007.34.5.529-532cry1ab蛋白离子交换色谱循环分离肽质量谱图分析 |
| spellingShingle | LUANMing-ming(栾明明) WUJian-min(邬建敏) YEQing-fu(叶庆富) Purification of Cry1Ab protein by multi-cycles ion-exchange chromatography and its peptide mass mapping(多次循环离子交换色谱纯化Cry1Ab蛋白及其肽质量谱图的分析) Zhejiang Daxue xuebao. Lixue ban cry1ab蛋白 离子交换色谱 循环分离 肽质量谱图分析 |
| title | Purification of Cry1Ab protein by multi-cycles ion-exchange chromatography and its peptide mass mapping(多次循环离子交换色谱纯化Cry1Ab蛋白及其肽质量谱图的分析) |
| title_full | Purification of Cry1Ab protein by multi-cycles ion-exchange chromatography and its peptide mass mapping(多次循环离子交换色谱纯化Cry1Ab蛋白及其肽质量谱图的分析) |
| title_fullStr | Purification of Cry1Ab protein by multi-cycles ion-exchange chromatography and its peptide mass mapping(多次循环离子交换色谱纯化Cry1Ab蛋白及其肽质量谱图的分析) |
| title_full_unstemmed | Purification of Cry1Ab protein by multi-cycles ion-exchange chromatography and its peptide mass mapping(多次循环离子交换色谱纯化Cry1Ab蛋白及其肽质量谱图的分析) |
| title_short | Purification of Cry1Ab protein by multi-cycles ion-exchange chromatography and its peptide mass mapping(多次循环离子交换色谱纯化Cry1Ab蛋白及其肽质量谱图的分析) |
| title_sort | purification of cry1ab protein by multi cycles ion exchange chromatography and its peptide mass mapping 多次循环离子交换色谱纯化cry1ab蛋白及其肽质量谱图的分析 |
| topic | cry1ab蛋白 离子交换色谱 循环分离 肽质量谱图分析 |
| url | https://doi.org/zjup/1008-9497.2007.34.5.529-532 |
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