Antibacterial and Antibiofilm Effects of Lactobacilli Strains against Clinical Isolates of <i>Pseudomonas aeruginosa</i> under Conditions Relevant to Cystic Fibrosis
Therapy of lung infections sustained by <i>Pseudomonas aeruginosa</i> in cystic fibrosis (CF) patients is challenging due to the presence of a sticky mucus in the airways and the ability of the bacterium to form biofilm, which exhibits increased antibiotic tolerance. A lung-directed bact...
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MDPI AG
2023-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/7/1158 |
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| author | Giovanna Batoni Elisa Catelli Esingül Kaya Arianna Pompilio Marta Bianchi Emilia Ghelardi Giovanni Di Bonaventura Semih Esin Giuseppantonio Maisetta |
| author_facet | Giovanna Batoni Elisa Catelli Esingül Kaya Arianna Pompilio Marta Bianchi Emilia Ghelardi Giovanni Di Bonaventura Semih Esin Giuseppantonio Maisetta |
| author_sort | Giovanna Batoni |
| collection | DOAJ |
| description | Therapy of lung infections sustained by <i>Pseudomonas aeruginosa</i> in cystic fibrosis (CF) patients is challenging due to the presence of a sticky mucus in the airways and the ability of the bacterium to form biofilm, which exhibits increased antibiotic tolerance. A lung-directed bacteriotherapy through the airway administration of probiotics could represent an alternative approach to probiotic diet supplementation to improve the benefits and clinical outcomes of this kind of intervention in CF patients. This study aims to evaluate the ability of probiotic strains to grow in artificial sputum medium (ASM), mimicking the CF lung microenvironment, and to affect the planktonic and biofilm growth of CF clinical strains of <i>P. aeruginosa</i> in the same conditions. The results demonstrate that <i>Lacticaseibacillus rhamnosus</i> and <i>Lactiplantibacillus plantarum</i> (LP) can grow in ASM. LP inhibited the planktonic growth of <i>P. aeruginosa</i>, while both lactobacilli reduced the pre-formed biofilm of <i>P. aeruginosa</i>. Interestingly, LP was demonstrated to reduce the amount of polysaccharides in the extracellular matrix of <i>P. aeruginosa</i> biofilms and to potentiate the antibiofilm effects of tobramycin. Overall, the results indicated that LP is a promising candidate as an adjuvant in the antimicrobial therapy of <i>P. aeruginosa</i> infections in CF patients. |
| first_indexed | 2024-03-11T01:21:26Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T01:21:26Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-3c80534423164c4f932f2639a5e1301a2023-11-18T18:03:04ZengMDPI AGAntibiotics2079-63822023-07-01127115810.3390/antibiotics12071158Antibacterial and Antibiofilm Effects of Lactobacilli Strains against Clinical Isolates of <i>Pseudomonas aeruginosa</i> under Conditions Relevant to Cystic FibrosisGiovanna Batoni0Elisa Catelli1Esingül Kaya2Arianna Pompilio3Marta Bianchi4Emilia Ghelardi5Giovanni Di Bonaventura6Semih Esin7Giuseppantonio Maisetta8Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Medical, Oral, and Biotechnological Sciences, G. d’Annunzio University of Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Medical, Oral, and Biotechnological Sciences, G. d’Annunzio University of Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via S. Zeno 37, 56123 Pisa, ItalyTherapy of lung infections sustained by <i>Pseudomonas aeruginosa</i> in cystic fibrosis (CF) patients is challenging due to the presence of a sticky mucus in the airways and the ability of the bacterium to form biofilm, which exhibits increased antibiotic tolerance. A lung-directed bacteriotherapy through the airway administration of probiotics could represent an alternative approach to probiotic diet supplementation to improve the benefits and clinical outcomes of this kind of intervention in CF patients. This study aims to evaluate the ability of probiotic strains to grow in artificial sputum medium (ASM), mimicking the CF lung microenvironment, and to affect the planktonic and biofilm growth of CF clinical strains of <i>P. aeruginosa</i> in the same conditions. The results demonstrate that <i>Lacticaseibacillus rhamnosus</i> and <i>Lactiplantibacillus plantarum</i> (LP) can grow in ASM. LP inhibited the planktonic growth of <i>P. aeruginosa</i>, while both lactobacilli reduced the pre-formed biofilm of <i>P. aeruginosa</i>. Interestingly, LP was demonstrated to reduce the amount of polysaccharides in the extracellular matrix of <i>P. aeruginosa</i> biofilms and to potentiate the antibiofilm effects of tobramycin. Overall, the results indicated that LP is a promising candidate as an adjuvant in the antimicrobial therapy of <i>P. aeruginosa</i> infections in CF patients.https://www.mdpi.com/2079-6382/12/7/1158lactobacilli<i>Pseudomonas aeruginosa</i>cystic fibrosisartificial sputum mediumbiofilmtobramycin |
| spellingShingle | Giovanna Batoni Elisa Catelli Esingül Kaya Arianna Pompilio Marta Bianchi Emilia Ghelardi Giovanni Di Bonaventura Semih Esin Giuseppantonio Maisetta Antibacterial and Antibiofilm Effects of Lactobacilli Strains against Clinical Isolates of <i>Pseudomonas aeruginosa</i> under Conditions Relevant to Cystic Fibrosis Antibiotics lactobacilli <i>Pseudomonas aeruginosa</i> cystic fibrosis artificial sputum medium biofilm tobramycin |
| title | Antibacterial and Antibiofilm Effects of Lactobacilli Strains against Clinical Isolates of <i>Pseudomonas aeruginosa</i> under Conditions Relevant to Cystic Fibrosis |
| title_full | Antibacterial and Antibiofilm Effects of Lactobacilli Strains against Clinical Isolates of <i>Pseudomonas aeruginosa</i> under Conditions Relevant to Cystic Fibrosis |
| title_fullStr | Antibacterial and Antibiofilm Effects of Lactobacilli Strains against Clinical Isolates of <i>Pseudomonas aeruginosa</i> under Conditions Relevant to Cystic Fibrosis |
| title_full_unstemmed | Antibacterial and Antibiofilm Effects of Lactobacilli Strains against Clinical Isolates of <i>Pseudomonas aeruginosa</i> under Conditions Relevant to Cystic Fibrosis |
| title_short | Antibacterial and Antibiofilm Effects of Lactobacilli Strains against Clinical Isolates of <i>Pseudomonas aeruginosa</i> under Conditions Relevant to Cystic Fibrosis |
| title_sort | antibacterial and antibiofilm effects of lactobacilli strains against clinical isolates of i pseudomonas aeruginosa i under conditions relevant to cystic fibrosis |
| topic | lactobacilli <i>Pseudomonas aeruginosa</i> cystic fibrosis artificial sputum medium biofilm tobramycin |
| url | https://www.mdpi.com/2079-6382/12/7/1158 |
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