Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage
Refrigerated storage is widely applied in order to maintain peach quality but it can also induce chilling injuries (CIs) such as flesh browning and bleeding, and mealiness. Peach fruit from three cultivars (‘Red Haven’, RH, ‘Regina di Londa’, RL, and ‘Flaminia’, FL) were stored for 4 weeks under low...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-05-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.00706/full |
| _version_ | 1819075756160974848 |
|---|---|
| author | Stefano Brizzolara Maarten Hertog Roberta Tosetti Bart Nicolai Bart Nicolai Pietro Tonutti |
| author_facet | Stefano Brizzolara Maarten Hertog Roberta Tosetti Bart Nicolai Bart Nicolai Pietro Tonutti |
| author_sort | Stefano Brizzolara |
| collection | DOAJ |
| description | Refrigerated storage is widely applied in order to maintain peach quality but it can also induce chilling injuries (CIs) such as flesh browning and bleeding, and mealiness. Peach fruit from three cultivars (‘Red Haven’, RH, ‘Regina di Londa’, RL, and ‘Flaminia’, FL) were stored for 4 weeks under low temperatures (0.5 and 5.5°C). GC-MS was employed to study changes in both metabolome and volatilome induced by cold storage in the mesocarp. CIs were assessed both at the end of each week of storage and after subsequent shelf-life (SL) at 20°C. Flesh browning and mealiness appeared to be more related to 5.5°C storage, while flesh bleeding revealed high incidence following 0.5°C storage. Compared to RL and FL, RH showed a marked lower incidence of CIs. Multivariate statistical analyses indicate that RH peaches indeed differ from RL and FL in particular when considering data from samples collected at the end of the cold storage. Common and divergent responses have been identified in terms of metabolic responses to the applied low temperatures. In all three cultivars raffinose, glucose-6P, fucose, xylose, sorbitol, GABA, epicatechin, catechin, and putrescine markedly increased during cold storage, while citramalic, glucuronic, mucic and shikimic acids decreased. Among volatile organic compounds (VOCs), aldehydes and alcohols generally accumulated more under low temperature conditions while esters and lactones evolved during subsequent SL. The main cultivar differences developed after cold storage during SL although some common responses (e.g., an increased production of ethyl acetate) were observed. The lower levels of flesh browning and bleeding displayed by RH peaches were related to compounds with antioxidant activity, or acting as osmotic protectants and membrane stabilizer. Indeed, RH showed higher levels of amino acids and urea, together with a marked increase in putrescine, sorbitol, maltitol, myoinositol and sucrose detected during storage and SL. |
| first_indexed | 2024-12-21T18:30:28Z |
| format | Article |
| id | doaj.art-3c80e04d4b0149c9b63405877a7a767d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T18:30:28Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-3c80e04d4b0149c9b63405877a7a767d2022-12-21T18:54:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-05-01910.3389/fpls.2018.00706373893Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold StorageStefano Brizzolara0Maarten Hertog1Roberta Tosetti2Bart Nicolai3Bart Nicolai4Pietro Tonutti5Istituto di Scienze della Vita, Scuola Superiore Sant’Anna, Pisa, ItalyDivision of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST), KU Leuven, Leuven, BelgiumIstituto di Scienze della Vita, Scuola Superiore Sant’Anna, Pisa, ItalyDivision of Mechatronics, Biostatistics and Sensors (MeBioS), Department of Biosystems (BIOSYST), KU Leuven, Leuven, BelgiumFlanders Centre of Postharvest Technology (VCBT), Leuven, BelgiumIstituto di Scienze della Vita, Scuola Superiore Sant’Anna, Pisa, ItalyRefrigerated storage is widely applied in order to maintain peach quality but it can also induce chilling injuries (CIs) such as flesh browning and bleeding, and mealiness. Peach fruit from three cultivars (‘Red Haven’, RH, ‘Regina di Londa’, RL, and ‘Flaminia’, FL) were stored for 4 weeks under low temperatures (0.5 and 5.5°C). GC-MS was employed to study changes in both metabolome and volatilome induced by cold storage in the mesocarp. CIs were assessed both at the end of each week of storage and after subsequent shelf-life (SL) at 20°C. Flesh browning and mealiness appeared to be more related to 5.5°C storage, while flesh bleeding revealed high incidence following 0.5°C storage. Compared to RL and FL, RH showed a marked lower incidence of CIs. Multivariate statistical analyses indicate that RH peaches indeed differ from RL and FL in particular when considering data from samples collected at the end of the cold storage. Common and divergent responses have been identified in terms of metabolic responses to the applied low temperatures. In all three cultivars raffinose, glucose-6P, fucose, xylose, sorbitol, GABA, epicatechin, catechin, and putrescine markedly increased during cold storage, while citramalic, glucuronic, mucic and shikimic acids decreased. Among volatile organic compounds (VOCs), aldehydes and alcohols generally accumulated more under low temperature conditions while esters and lactones evolved during subsequent SL. The main cultivar differences developed after cold storage during SL although some common responses (e.g., an increased production of ethyl acetate) were observed. The lower levels of flesh browning and bleeding displayed by RH peaches were related to compounds with antioxidant activity, or acting as osmotic protectants and membrane stabilizer. Indeed, RH showed higher levels of amino acids and urea, together with a marked increase in putrescine, sorbitol, maltitol, myoinositol and sucrose detected during storage and SL.https://www.frontiersin.org/article/10.3389/fpls.2018.00706/fullchilling injurycold storagecorrelation analysismesocarpmetabolomicsPrunus persica |
| spellingShingle | Stefano Brizzolara Maarten Hertog Roberta Tosetti Bart Nicolai Bart Nicolai Pietro Tonutti Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage Frontiers in Plant Science chilling injury cold storage correlation analysis mesocarp metabolomics Prunus persica |
| title | Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage |
| title_full | Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage |
| title_fullStr | Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage |
| title_full_unstemmed | Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage |
| title_short | Metabolic Responses to Low Temperature of Three Peach Fruit Cultivars Differently Sensitive to Cold Storage |
| title_sort | metabolic responses to low temperature of three peach fruit cultivars differently sensitive to cold storage |
| topic | chilling injury cold storage correlation analysis mesocarp metabolomics Prunus persica |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.00706/full |
| work_keys_str_mv | AT stefanobrizzolara metabolicresponsestolowtemperatureofthreepeachfruitcultivarsdifferentlysensitivetocoldstorage AT maartenhertog metabolicresponsestolowtemperatureofthreepeachfruitcultivarsdifferentlysensitivetocoldstorage AT robertatosetti metabolicresponsestolowtemperatureofthreepeachfruitcultivarsdifferentlysensitivetocoldstorage AT bartnicolai metabolicresponsestolowtemperatureofthreepeachfruitcultivarsdifferentlysensitivetocoldstorage AT bartnicolai metabolicresponsestolowtemperatureofthreepeachfruitcultivarsdifferentlysensitivetocoldstorage AT pietrotonutti metabolicresponsestolowtemperatureofthreepeachfruitcultivarsdifferentlysensitivetocoldstorage |