Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK
Morchella angusticeps Peck, one of the most popular edible mushrooms, has attracted great attention due to its delicious taste and healthy properties. However, both its biological effects and the possible mechanism of action have not yet been known. We investigated the anti-proliferative activity of...
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| Format: | Article |
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Elsevier
2020-11-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311920633226 |
| _version_ | 1819065692621635584 |
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| author | Fu-hua LI Shao-jie ZHENG Ji-chun ZHAO Xia LIAO Su-rui WU Jian MING |
| author_facet | Fu-hua LI Shao-jie ZHENG Ji-chun ZHAO Xia LIAO Su-rui WU Jian MING |
| author_sort | Fu-hua LI |
| collection | DOAJ |
| description | Morchella angusticeps Peck, one of the most popular edible mushrooms, has attracted great attention due to its delicious taste and healthy properties. However, both its biological effects and the possible mechanism of action have not yet been known. We investigated the anti-proliferative activity of the phenolic extract derived from Morchella angusticeps Peck (MPE) against HepG2 human hepatocellular carcinoma cells. Results showed that MPE at non-cytotoxicity doses significantly inhibited the proliferation of HepG2 cells in a dose-dependent manner with inhibitory rates ranging from 18 to 90% (P<0.01). The possible mechanism might be that MPE induced apoptosis through initiating the mitochondrial death pathway by regulating Bax, Bcl-2 and cleaved caspase-3. On the other hand, MPE might trigger cell cycle arrest at G0/G1/S phases by managing p21, Cyclin D1, cyclin-dependent kinases-4 (CDK4) and proliferating cell nuclear antigen (PCNA). Additionally, MPE downregulated TRAF-2 and p-p53, while upregulated p-ASK1 and p-p38. Therefore, it could be inferred that MPE might induce the anti-proliferative function to HepG2 cells through the p38/MAPK signal transduction pathway. |
| first_indexed | 2024-12-21T15:50:30Z |
| format | Article |
| id | doaj.art-3c88c648e1b74510b6c0582b509d913e |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-21T15:50:30Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-3c88c648e1b74510b6c0582b509d913e2022-12-21T18:58:14ZengElsevierJournal of Integrative Agriculture2095-31192020-11-01191128292838Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPKFu-hua LI0Shao-jie ZHENG1Ji-chun ZHAO2Xia LIAO3Su-rui WU4Jian MING5College of Food Science, Southwest University, Chongqing 400715, P.R.China; Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, P.R.ChinaCollege of Food Science, Southwest University, Chongqing 400715, P.R.ChinaCollege of Food Science, Southwest University, Chongqing 400715, P.R.China; Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, P.R.ChinaCollege of Food Science, Southwest University, Chongqing 400715, P.R.ChinaKunming Edible Fungi Institute, All China Federation of Supply and Marketing Cooperatives, Kunming 650223, P.R.ChinaCollege of Food Science, Southwest University, Chongqing 400715, P.R.China; Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, P.R.China; Correspondence MING Jian, Tel/Fax: +86-23-68251947Morchella angusticeps Peck, one of the most popular edible mushrooms, has attracted great attention due to its delicious taste and healthy properties. However, both its biological effects and the possible mechanism of action have not yet been known. We investigated the anti-proliferative activity of the phenolic extract derived from Morchella angusticeps Peck (MPE) against HepG2 human hepatocellular carcinoma cells. Results showed that MPE at non-cytotoxicity doses significantly inhibited the proliferation of HepG2 cells in a dose-dependent manner with inhibitory rates ranging from 18 to 90% (P<0.01). The possible mechanism might be that MPE induced apoptosis through initiating the mitochondrial death pathway by regulating Bax, Bcl-2 and cleaved caspase-3. On the other hand, MPE might trigger cell cycle arrest at G0/G1/S phases by managing p21, Cyclin D1, cyclin-dependent kinases-4 (CDK4) and proliferating cell nuclear antigen (PCNA). Additionally, MPE downregulated TRAF-2 and p-p53, while upregulated p-ASK1 and p-p38. Therefore, it could be inferred that MPE might induce the anti-proliferative function to HepG2 cells through the p38/MAPK signal transduction pathway.http://www.sciencedirect.com/science/article/pii/S2095311920633226Morchella angusticeps Peckphenolic extractcell cycleapoptosisanti-proliferation |
| spellingShingle | Fu-hua LI Shao-jie ZHENG Ji-chun ZHAO Xia LIAO Su-rui WU Jian MING Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK Journal of Integrative Agriculture Morchella angusticeps Peck phenolic extract cell cycle apoptosis anti-proliferation |
| title | Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK |
| title_full | Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK |
| title_fullStr | Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK |
| title_full_unstemmed | Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK |
| title_short | Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK |
| title_sort | phenolic extract of morchella angusticeps peck inhibited the proliferation of hepg2 cells in vitro by inducing the signal transduction pathway of p38 mapk |
| topic | Morchella angusticeps Peck phenolic extract cell cycle apoptosis anti-proliferation |
| url | http://www.sciencedirect.com/science/article/pii/S2095311920633226 |
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