Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus)
Glucocorticoids have a wide range of pharmacological activities. Generally speaking, the steroid drugs, such as dexamethasone (DEX) can have severe side effects on the histology of different organs. In fact, glucocorticoids have been known as powerful medicines which can cure inflammation and work w...
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Razi Vaccine and Serum Research Institute
2022-02-01
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| Series: | Archives of Razi Institute |
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| Online Access: | https://archrazi.areeo.ac.ir/article_125234_07b9ddaa4fa973223679971bbdf33466.pdf |
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| author | N Hammadi Jasim D Adel Kareem M. F Majeed AL Ali B. A Abbas |
| author_facet | N Hammadi Jasim D Adel Kareem M. F Majeed AL Ali B. A Abbas |
| author_sort | N Hammadi Jasim |
| collection | DOAJ |
| description | Glucocorticoids have a wide range of pharmacological activities. Generally speaking, the steroid drugs, such as dexamethasone (DEX) can have severe side effects on the histology of different organs. In fact, glucocorticoids have been known as powerful medicines which can cure inflammation and work with the immune system to treat a wide range of health problems. Therefore, this study aimed to investigate the effects of DEX on the histological changes of the liver and kidney, as well as blood biochemical parameters. In total, 13 specific pathogen-free male Lepus cuniculus rabbits aged 8-10 months old, with a mean weight of 1.12±0.13 kg were randomly divided into three groups. Group I (n=3) did not receive DEX, and they only received saline solution as a placebo (control). In Group II (n=5), the animals received 0.25 mg DEX/kg body weight/day for a period of 56 days, and the animals in Group III (n=5) received 0.5 mg DEX/kg body weight/day for 56 days. Blood was aspirated from the rabbit's marginal ear vein. All blood samples were centrifuged at 3000×g for 10 min to separate serum samples. Blood lipids and trace elements (zinc, copper, calcium, and iron) were measured. The microscopical analyses of the liver and kidney tissues were performed through the observation of the histological changes in the tissues. The results showed a significant (P≤0.05) decrease in the body and organ weight, as well as serum concentrations for the trace elements. On the other hand, lipid profile showed a significant increase (P≤0.05) in cholesterol, triglycerides, and low-density lipoprotein. However, a significant decrease was recorded in high-density lipoprotein in both treated groups with DEX, compared to the control group. The results of the histological evaluation showed some degrees of degeneration, necrosis, cell vacuolation, and lymphocyte infiltration in the kidney and liver tissues in the treatment groups. |
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| language | English |
| last_indexed | 2024-04-13T22:02:52Z |
| publishDate | 2022-02-01 |
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| series | Archives of Razi Institute |
| spelling | doaj.art-3c8a971bf521446699bfce09842bc3d22022-12-22T02:28:02ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722022-02-0177133334310.22092/ari.2021.356468.1847125234Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus)N Hammadi Jasim0D Adel Kareem1M. F Majeed AL Ali2B. A Abbas3Department of Physiology and Pharmacology and Chemistry, College of Veterinary Medicine, University of Basrah, Basrah, IraqDepartment of Anatomy and Histology, College of Veterinary Medicine, University of Basrah, Basrah, IraqDepartment of Anatomy and Histology, College of Veterinary Medicine, University of Basrah, Basrah, IraqDepartment of Microbiology and Parasitology, College of Veterinary Medicine, University of Basrah, Basrah, IraqGlucocorticoids have a wide range of pharmacological activities. Generally speaking, the steroid drugs, such as dexamethasone (DEX) can have severe side effects on the histology of different organs. In fact, glucocorticoids have been known as powerful medicines which can cure inflammation and work with the immune system to treat a wide range of health problems. Therefore, this study aimed to investigate the effects of DEX on the histological changes of the liver and kidney, as well as blood biochemical parameters. In total, 13 specific pathogen-free male Lepus cuniculus rabbits aged 8-10 months old, with a mean weight of 1.12±0.13 kg were randomly divided into three groups. Group I (n=3) did not receive DEX, and they only received saline solution as a placebo (control). In Group II (n=5), the animals received 0.25 mg DEX/kg body weight/day for a period of 56 days, and the animals in Group III (n=5) received 0.5 mg DEX/kg body weight/day for 56 days. Blood was aspirated from the rabbit's marginal ear vein. All blood samples were centrifuged at 3000×g for 10 min to separate serum samples. Blood lipids and trace elements (zinc, copper, calcium, and iron) were measured. The microscopical analyses of the liver and kidney tissues were performed through the observation of the histological changes in the tissues. The results showed a significant (P≤0.05) decrease in the body and organ weight, as well as serum concentrations for the trace elements. On the other hand, lipid profile showed a significant increase (P≤0.05) in cholesterol, triglycerides, and low-density lipoprotein. However, a significant decrease was recorded in high-density lipoprotein in both treated groups with DEX, compared to the control group. The results of the histological evaluation showed some degrees of degeneration, necrosis, cell vacuolation, and lymphocyte infiltration in the kidney and liver tissues in the treatment groups.https://archrazi.areeo.ac.ir/article_125234_07b9ddaa4fa973223679971bbdf33466.pdfdexamethasoneessential mineralhistologylipid profile |
| spellingShingle | N Hammadi Jasim D Adel Kareem M. F Majeed AL Ali B. A Abbas Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus) Archives of Razi Institute dexamethasone essential mineral histology lipid profile |
| title | Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus) |
| title_full | Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus) |
| title_fullStr | Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus) |
| title_full_unstemmed | Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus) |
| title_short | Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus) |
| title_sort | effect of long term treatment with dexamethasone on the liver and kidney histopathology as well as blood biochemistry in male rabbits lepus cuniculus |
| topic | dexamethasone essential mineral histology lipid profile |
| url | https://archrazi.areeo.ac.ir/article_125234_07b9ddaa4fa973223679971bbdf33466.pdf |
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