Structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysis
Abstract Cellulose I is not completely saccharified to glucose at a low cellulase concentration. In this study, sugarcane cellulose saccharification residues were investigated. Transmission electron microscopy images indicated that the cellulose microfibrils became shorter in the early stages of sac...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-02-01
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| Series: | Journal of Wood Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s10086-020-01859-2 |
| _version_ | 1818406801976066048 |
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| author | Makiko Imai Yoshiki Horikawa Shingo Kiyoto Tomoya Imai Junji Sugiyama |
| author_facet | Makiko Imai Yoshiki Horikawa Shingo Kiyoto Tomoya Imai Junji Sugiyama |
| author_sort | Makiko Imai |
| collection | DOAJ |
| description | Abstract Cellulose I is not completely saccharified to glucose at a low cellulase concentration. In this study, sugarcane cellulose saccharification residues were investigated. Transmission electron microscopy images indicated that the cellulose microfibrils became shorter in the early stages of saccharification and gradually became narrower. The degree of polymerization also decreased in the early stages of saccharification. Cellulose saccharification residues were deuterated by immersing them in deuterium oxide. Infra-red spectra of the deuterated residues indicated that the deuterated hydroxyl group ratio decreased as saccharification progressed. This indicated that cellulose microfibrils were hydrolyzed in their hydrophobic planes by cellulase as if the surfaces were scraped. The increase of hydrophobic planes caused microfibril aggregation, poor accessibility of cellulase to the microfibrils, and a low saccharification rate. |
| first_indexed | 2024-12-14T09:17:43Z |
| format | Article |
| id | doaj.art-3c8ba5b64caa429e92c68b00639ac511 |
| institution | Directory Open Access Journal |
| issn | 1435-0211 1611-4663 |
| language | English |
| last_indexed | 2024-12-14T09:17:43Z |
| publishDate | 2020-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Wood Science |
| spelling | doaj.art-3c8ba5b64caa429e92c68b00639ac5112022-12-21T23:08:24ZengSpringerOpenJournal of Wood Science1435-02111611-46632020-02-016611810.1186/s10086-020-01859-2Structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysisMakiko Imai0Yoshiki Horikawa1Shingo Kiyoto2Tomoya Imai3Junji Sugiyama4Research Institute for Sustainable Humanosphere, Kyoto UniversityInstitute of Agriculture, Tokyo University of Agriculture and TechnologyResearch Institute for Sustainable Humanosphere, Kyoto UniversityResearch Institute for Sustainable Humanosphere, Kyoto UniversityResearch Institute for Sustainable Humanosphere, Kyoto UniversityAbstract Cellulose I is not completely saccharified to glucose at a low cellulase concentration. In this study, sugarcane cellulose saccharification residues were investigated. Transmission electron microscopy images indicated that the cellulose microfibrils became shorter in the early stages of saccharification and gradually became narrower. The degree of polymerization also decreased in the early stages of saccharification. Cellulose saccharification residues were deuterated by immersing them in deuterium oxide. Infra-red spectra of the deuterated residues indicated that the deuterated hydroxyl group ratio decreased as saccharification progressed. This indicated that cellulose microfibrils were hydrolyzed in their hydrophobic planes by cellulase as if the surfaces were scraped. The increase of hydrophobic planes caused microfibril aggregation, poor accessibility of cellulase to the microfibrils, and a low saccharification rate.https://doi.org/10.1186/s10086-020-01859-2CelluloseCellulaseDeuterationHydrophobic planeHydrophilic planeSAXS |
| spellingShingle | Makiko Imai Yoshiki Horikawa Shingo Kiyoto Tomoya Imai Junji Sugiyama Structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysis Journal of Wood Science Cellulose Cellulase Deuteration Hydrophobic plane Hydrophilic plane SAXS |
| title | Structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysis |
| title_full | Structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysis |
| title_fullStr | Structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysis |
| title_full_unstemmed | Structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysis |
| title_short | Structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysis |
| title_sort | structural changes in sugarcane bagasse cellulose caused by enzymatic hydrolysis |
| topic | Cellulose Cellulase Deuteration Hydrophobic plane Hydrophilic plane SAXS |
| url | https://doi.org/10.1186/s10086-020-01859-2 |
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