Antimicrobial activities of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L.
The extracts of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L. were investigated for antimicrobial activities against the following common microorganisms using an agar diffusion method: Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Proteus vulgaris, Saccharomyces cerevisiae,...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Julius Kühn-Institut
2013-05-01
|
| Series: | Journal of Applied Botany and Food Quality |
| Subjects: | |
| Online Access: | https://ojs.openagrar.de/index.php/JABFQ/article/view/2300 |
| _version_ | 1818413099133173760 |
|---|---|
| author | Jin Cai Shulian Xie Jia Feng |
| author_facet | Jin Cai Shulian Xie Jia Feng |
| author_sort | Jin Cai |
| collection | DOAJ |
| description | The extracts of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L. were investigated for antimicrobial activities against the following common microorganisms using an agar diffusion method: Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Proteus vulgaris, Saccharomyces cerevisiae, and Candida albicans. The extracts strongly inhibited the growth of Gram-positive bacteria, but did not show inhibitory activity against Gram-negative bacteria and yeast. In this study, novel methods for extraction of antimicrobial substances from N. obtusa and C. vulgaris were reported. The optimum extraction conditions were investigated using single factor experimental design and L273(13) orthogonal experimental design. Results showed that the optimum extraction conditions for N. obtusa are: solid to liquid ratio 1:15, temperature 85°C, ethanol concentration 50%, extraction time 6h; and the optimum extraction conditions for C. vulgaris are: solid to liquid ratio 1:15, temperature 85°C, ethanol concentration 70%, extraction time 10h. Stability study demonstrated neuter and alkaline conditions enhanced the antimicrobial activities from N. obtusa and C. vulgaris, and both extracts were stable under ultraviolet (UV). These data suggest that extracts from both N. obtusa and C. vulgaris might be of potential use as bactericidal agents. |
| first_indexed | 2024-12-14T10:57:49Z |
| format | Article |
| id | doaj.art-3c8c3594163f47b08dc734cf4f85e583 |
| institution | Directory Open Access Journal |
| issn | 1613-9216 1439-040X |
| language | English |
| last_indexed | 2024-12-14T10:57:49Z |
| publishDate | 2013-05-01 |
| publisher | Julius Kühn-Institut |
| record_format | Article |
| series | Journal of Applied Botany and Food Quality |
| spelling | doaj.art-3c8c3594163f47b08dc734cf4f85e5832022-12-21T23:04:52ZengJulius Kühn-InstitutJournal of Applied Botany and Food Quality1613-92161439-040X2013-05-0186110.5073/JABFQ.2013.086.0042300Antimicrobial activities of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L.Jin CaiShulian XieJia FengThe extracts of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L. were investigated for antimicrobial activities against the following common microorganisms using an agar diffusion method: Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Proteus vulgaris, Saccharomyces cerevisiae, and Candida albicans. The extracts strongly inhibited the growth of Gram-positive bacteria, but did not show inhibitory activity against Gram-negative bacteria and yeast. In this study, novel methods for extraction of antimicrobial substances from N. obtusa and C. vulgaris were reported. The optimum extraction conditions were investigated using single factor experimental design and L273(13) orthogonal experimental design. Results showed that the optimum extraction conditions for N. obtusa are: solid to liquid ratio 1:15, temperature 85°C, ethanol concentration 50%, extraction time 6h; and the optimum extraction conditions for C. vulgaris are: solid to liquid ratio 1:15, temperature 85°C, ethanol concentration 70%, extraction time 10h. Stability study demonstrated neuter and alkaline conditions enhanced the antimicrobial activities from N. obtusa and C. vulgaris, and both extracts were stable under ultraviolet (UV). These data suggest that extracts from both N. obtusa and C. vulgaris might be of potential use as bactericidal agents.https://ojs.openagrar.de/index.php/JABFQ/article/view/2300Antimicrobial activityChara vulgaris L.Extraction optimizationExtracts stabilityNitellopsis obtusa (Desvaux) Groves |
| spellingShingle | Jin Cai Shulian Xie Jia Feng Antimicrobial activities of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L. Journal of Applied Botany and Food Quality Antimicrobial activity Chara vulgaris L. Extraction optimization Extracts stability Nitellopsis obtusa (Desvaux) Groves |
| title | Antimicrobial activities of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L. |
| title_full | Antimicrobial activities of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L. |
| title_fullStr | Antimicrobial activities of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L. |
| title_full_unstemmed | Antimicrobial activities of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L. |
| title_short | Antimicrobial activities of Nitellopsis obtusa (Desvaux) Groves and Chara vulgaris L. |
| title_sort | antimicrobial activities of nitellopsis obtusa desvaux groves and chara vulgaris l |
| topic | Antimicrobial activity Chara vulgaris L. Extraction optimization Extracts stability Nitellopsis obtusa (Desvaux) Groves |
| url | https://ojs.openagrar.de/index.php/JABFQ/article/view/2300 |
| work_keys_str_mv | AT jincai antimicrobialactivitiesofnitellopsisobtusadesvauxgrovesandcharavulgarisl AT shulianxie antimicrobialactivitiesofnitellopsisobtusadesvauxgrovesandcharavulgarisl AT jiafeng antimicrobialactivitiesofnitellopsisobtusadesvauxgrovesandcharavulgarisl |