Damage of <it>Streptococcus mutans </it>biofilms by carolacton, a secondary metabolite from the myxobacterium <it>Sorangium cellulosum</it>
<p>Abstract</p> <p>Background</p> <p><it>Streptococcus mutans </it>is a major pathogen in human dental caries. One of its important virulence properties is the ability to form biofilms (dental plaque) on tooth surfaces. Eradication of such biofilms is extrem...
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| Format: | Article |
| Language: | English |
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BMC
2010-07-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/199 |
| _version_ | 1818492899000582144 |
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| author | Irschik Herbert Schummer Dietmar Lemme André Dötsch Andreas Reck Michael Kunze Brigitte Steinmetz Heinrich Wagner-Döbler Irene |
| author_facet | Irschik Herbert Schummer Dietmar Lemme André Dötsch Andreas Reck Michael Kunze Brigitte Steinmetz Heinrich Wagner-Döbler Irene |
| author_sort | Irschik Herbert |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Streptococcus mutans </it>is a major pathogen in human dental caries. One of its important virulence properties is the ability to form biofilms (dental plaque) on tooth surfaces. Eradication of such biofilms is extremely difficult. We therefore screened a library of secondary metabolites from myxobacteria for their ability to damage biofilms of <it>S. mutans</it>.</p> <p>Results</p> <p>Here we show that carolacton, a secondary metabolite isolated from <it>Sorangium cellulosum</it>, has high antibacterial activity against biofilms of <it>S. mutans</it>. Planktonic growth of bacteria was only slightly impaired and no acute cytotoxicity against mouse fibroblasts could be observed. Carolacton caused death of <it>S. mutans </it>biofilm cells, elongation of cell chains, and changes in cell morphology. At a concentration of 10 nM carolacton, biofilm damage was already at 35% under anaerobic conditions. A knock-out mutant for <it>comD</it>, encoding a histidine kinase specific for the competence stimulating peptide (CSP), was slightly less sensitive to carolacton than the wildtype. Expression of the competence related alternate sigma factor ComX was strongly reduced by carolacton, as determined by a <it>pcomX </it>luciferase reporter strain.</p> <p>Conclusions</p> <p>Carolacton possibly interferes with the density dependent signalling systems in <it>S. mutans </it>and may represent a novel approach for the prevention of dental caries.</p> |
| first_indexed | 2024-12-10T17:48:20Z |
| format | Article |
| id | doaj.art-3c8c52debec84e00981aa3872d723061 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T17:48:20Z |
| publishDate | 2010-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-3c8c52debec84e00981aa3872d7230612022-12-22T01:39:09ZengBMCBMC Microbiology1471-21802010-07-0110119910.1186/1471-2180-10-199Damage of <it>Streptococcus mutans </it>biofilms by carolacton, a secondary metabolite from the myxobacterium <it>Sorangium cellulosum</it>Irschik HerbertSchummer DietmarLemme AndréDötsch AndreasReck MichaelKunze BrigitteSteinmetz HeinrichWagner-Döbler Irene<p>Abstract</p> <p>Background</p> <p><it>Streptococcus mutans </it>is a major pathogen in human dental caries. One of its important virulence properties is the ability to form biofilms (dental plaque) on tooth surfaces. Eradication of such biofilms is extremely difficult. We therefore screened a library of secondary metabolites from myxobacteria for their ability to damage biofilms of <it>S. mutans</it>.</p> <p>Results</p> <p>Here we show that carolacton, a secondary metabolite isolated from <it>Sorangium cellulosum</it>, has high antibacterial activity against biofilms of <it>S. mutans</it>. Planktonic growth of bacteria was only slightly impaired and no acute cytotoxicity against mouse fibroblasts could be observed. Carolacton caused death of <it>S. mutans </it>biofilm cells, elongation of cell chains, and changes in cell morphology. At a concentration of 10 nM carolacton, biofilm damage was already at 35% under anaerobic conditions. A knock-out mutant for <it>comD</it>, encoding a histidine kinase specific for the competence stimulating peptide (CSP), was slightly less sensitive to carolacton than the wildtype. Expression of the competence related alternate sigma factor ComX was strongly reduced by carolacton, as determined by a <it>pcomX </it>luciferase reporter strain.</p> <p>Conclusions</p> <p>Carolacton possibly interferes with the density dependent signalling systems in <it>S. mutans </it>and may represent a novel approach for the prevention of dental caries.</p>http://www.biomedcentral.com/1471-2180/10/199 |
| spellingShingle | Irschik Herbert Schummer Dietmar Lemme André Dötsch Andreas Reck Michael Kunze Brigitte Steinmetz Heinrich Wagner-Döbler Irene Damage of <it>Streptococcus mutans </it>biofilms by carolacton, a secondary metabolite from the myxobacterium <it>Sorangium cellulosum</it> BMC Microbiology |
| title | Damage of <it>Streptococcus mutans </it>biofilms by carolacton, a secondary metabolite from the myxobacterium <it>Sorangium cellulosum</it> |
| title_full | Damage of <it>Streptococcus mutans </it>biofilms by carolacton, a secondary metabolite from the myxobacterium <it>Sorangium cellulosum</it> |
| title_fullStr | Damage of <it>Streptococcus mutans </it>biofilms by carolacton, a secondary metabolite from the myxobacterium <it>Sorangium cellulosum</it> |
| title_full_unstemmed | Damage of <it>Streptococcus mutans </it>biofilms by carolacton, a secondary metabolite from the myxobacterium <it>Sorangium cellulosum</it> |
| title_short | Damage of <it>Streptococcus mutans </it>biofilms by carolacton, a secondary metabolite from the myxobacterium <it>Sorangium cellulosum</it> |
| title_sort | damage of it streptococcus mutans it biofilms by carolacton a secondary metabolite from the myxobacterium it sorangium cellulosum it |
| url | http://www.biomedcentral.com/1471-2180/10/199 |
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