High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil
Abstract Leishmaniasis is an anthropozoonosis with vector transmission, and knowledge regarding the occurrence of this parasitosis in sentinels can contribute to infection and disease control measures in humans. The objectives of this study were to evaluate the occurrence of Leishmania exposure and...
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Colégio Brasileiro de Parasitologia Veterinaria
2023-06-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612023000200405&lng=en&tlng=en |
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| author | Ana Carolina Cavallieri Débora Sayuri Katto Luciane Holsback Eloiza Teles Caldart Luana Zaboski Pena Pablo Menegon Castilho Fernanda Pinheiro Filgueiras Ellen de Souza Marquez Mariza Fordellone Rosa Cruz Kerlei Cristina Médici Regina Mitsuka-Breganó Italmar Teodorico Navarro |
| author_facet | Ana Carolina Cavallieri Débora Sayuri Katto Luciane Holsback Eloiza Teles Caldart Luana Zaboski Pena Pablo Menegon Castilho Fernanda Pinheiro Filgueiras Ellen de Souza Marquez Mariza Fordellone Rosa Cruz Kerlei Cristina Médici Regina Mitsuka-Breganó Italmar Teodorico Navarro |
| author_sort | Ana Carolina Cavallieri |
| collection | DOAJ |
| description | Abstract Leishmaniasis is an anthropozoonosis with vector transmission, and knowledge regarding the occurrence of this parasitosis in sentinels can contribute to infection and disease control measures in humans. The objectives of this study were to evaluate the occurrence of Leishmania exposure and infection in dogs from urban and rural areas in the North Pioneer Mesoregion of the state of Paraná, to evaluate possible risk factors, and to analyze the statistical agreement between the serological techniques that were used. Using a convenience sampling, serum and whole blood samples were collected to perform serological and molecular assays, respectively. The enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT) identified 29/204 (14.2%) and 20/204 (9.8%) seropositive dogs, respectively. Five dogs (2.4%) were seropositive for both serological tests, and four dogs presented high titers in the IFAT. None of the samples tested positive for Leishmania spp. DNA according to polymerase chain reaction analysis. No factors were significantly associated with infection. Leishmania parasites circulate in urban and rural dogs in the North Pioneer Mesoregion of the state of Paraná. Despite the absence of clinical cases, seropositive animals with high antibody titers should serve as a warning to the local population that should be properly informed regarding the prevention. |
| first_indexed | 2024-03-13T07:07:24Z |
| format | Article |
| id | doaj.art-3c8d07cc1e0541829eae868df772b2fd |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-03-13T07:07:24Z |
| publishDate | 2023-06-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-3c8d07cc1e0541829eae868df772b2fd2023-06-06T07:34:24ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612023-06-0132210.1590/s1984-29612023023High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, BrazilAna Carolina Cavallierihttps://orcid.org/0000-0002-1852-0181Débora Sayuri KattoLuciane HolsbackEloiza Teles CaldartLuana Zaboski PenaPablo Menegon CastilhoFernanda Pinheiro FilgueirasEllen de Souza MarquezMariza Fordellone Rosa CruzKerlei Cristina MédiciRegina Mitsuka-BreganóItalmar Teodorico NavarroAbstract Leishmaniasis is an anthropozoonosis with vector transmission, and knowledge regarding the occurrence of this parasitosis in sentinels can contribute to infection and disease control measures in humans. The objectives of this study were to evaluate the occurrence of Leishmania exposure and infection in dogs from urban and rural areas in the North Pioneer Mesoregion of the state of Paraná, to evaluate possible risk factors, and to analyze the statistical agreement between the serological techniques that were used. Using a convenience sampling, serum and whole blood samples were collected to perform serological and molecular assays, respectively. The enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT) identified 29/204 (14.2%) and 20/204 (9.8%) seropositive dogs, respectively. Five dogs (2.4%) were seropositive for both serological tests, and four dogs presented high titers in the IFAT. None of the samples tested positive for Leishmania spp. DNA according to polymerase chain reaction analysis. No factors were significantly associated with infection. Leishmania parasites circulate in urban and rural dogs in the North Pioneer Mesoregion of the state of Paraná. Despite the absence of clinical cases, seropositive animals with high antibody titers should serve as a warning to the local population that should be properly informed regarding the prevention.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612023000200405&lng=en&tlng=enLeishmania braziliensisELISAIFATcutaneous leishmaniasis |
| spellingShingle | Ana Carolina Cavallieri Débora Sayuri Katto Luciane Holsback Eloiza Teles Caldart Luana Zaboski Pena Pablo Menegon Castilho Fernanda Pinheiro Filgueiras Ellen de Souza Marquez Mariza Fordellone Rosa Cruz Kerlei Cristina Médici Regina Mitsuka-Breganó Italmar Teodorico Navarro High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil Revista Brasileira de Parasitologia Veterinária Leishmania braziliensis ELISA IFAT cutaneous leishmaniasis |
| title | High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil |
| title_full | High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil |
| title_fullStr | High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil |
| title_full_unstemmed | High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil |
| title_short | High titers of anti-Leishmania spp. antibodies in apparently healthy dogs in the North Pioneer Mesoregion of the state of Paraná, Brazil |
| title_sort | high titers of anti leishmania spp antibodies in apparently healthy dogs in the north pioneer mesoregion of the state of parana brazil |
| topic | Leishmania braziliensis ELISA IFAT cutaneous leishmaniasis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612023000200405&lng=en&tlng=en |
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