Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine
The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spec...
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| Format: | Article |
| Language: | English |
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Hitit University
2023-03-01
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| Series: | Hittite Journal of Science and Engineering |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/2914196 |
| _version_ | 1827796215677321216 |
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| author | Aykut Arif Topçu |
| author_facet | Aykut Arif Topçu |
| author_sort | Aykut Arif Topçu |
| collection | DOAJ |
| description | The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution.
The maximum adsorption capacity (Qmax) of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin (BSA). Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles. |
| first_indexed | 2024-03-11T19:03:10Z |
| format | Article |
| id | doaj.art-3c8e37261a0b4f129fc4ff6ceb0964ad |
| institution | Directory Open Access Journal |
| issn | 2148-4171 |
| language | English |
| last_indexed | 2024-03-11T19:03:10Z |
| publishDate | 2023-03-01 |
| publisher | Hitit University |
| record_format | Article |
| series | Hittite Journal of Science and Engineering |
| spelling | doaj.art-3c8e37261a0b4f129fc4ff6ceb0964ad2023-10-10T11:17:27ZengHitit UniversityHittite Journal of Science and Engineering2148-41712023-03-01101778210.17350/HJSE19030000294150Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamineAykut Arif Topçu0Aksaray ÜniversitesiThe surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the poly(hydroxyethyl methacrylate) [p(HEMA)] cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution. The maximum adsorption capacity (Qmax) of lysozyme was found as 108.9 mg/g and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin (BSA). Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.https://dergipark.org.tr/tr/download/article-file/2914196lysozymeadsorptionmolecular imprintingp(hema)dopaminecryogel.lysozymeadsorptionmolecular imprintingp(hema)dopaminecryogel. |
| spellingShingle | Aykut Arif Topçu Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine Hittite Journal of Science and Engineering lysozyme adsorption molecular imprinting p(hema) dopamine cryogel. lysozyme adsorption molecular imprinting p(hema) dopamine cryogel. |
| title | Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine |
| title_full | Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine |
| title_fullStr | Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine |
| title_full_unstemmed | Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine |
| title_short | Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine |
| title_sort | lysozyme imprinted poly hydroxyethyl methacrylate cryogel membrane with the assistance of polydopamine |
| topic | lysozyme adsorption molecular imprinting p(hema) dopamine cryogel. lysozyme adsorption molecular imprinting p(hema) dopamine cryogel. |
| url | https://dergipark.org.tr/tr/download/article-file/2914196 |
| work_keys_str_mv | AT aykutariftopcu lysozymeimprintedpolyhydroxyethylmethacrylatecryogelmembranewiththeassistanceofpolydopamine |