Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol<sup>3</sup>
The microbiome of the colon is characterized by its great diversity. This varies not only intra- but also interindividually and is influenced by endogenous and exogenous factors, such as dietary and lifestyle factors. The aim of this work was to investigate the extent to which the degradation of the...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/16/8/1128 |
| _version_ | 1827728897147404288 |
|---|---|
| author | Dariah-Sohreh Seradj Regine Beeck Annika Haase Julius Krause Philipp Schick Werner Weitschies |
| author_facet | Dariah-Sohreh Seradj Regine Beeck Annika Haase Julius Krause Philipp Schick Werner Weitschies |
| author_sort | Dariah-Sohreh Seradj |
| collection | DOAJ |
| description | The microbiome of the colon is characterized by its great diversity. This varies not only intra- but also interindividually and is influenced by endogenous and exogenous factors, such as dietary and lifestyle factors. The aim of this work was to investigate the extent to which the degradation of the drug sulfasalazine is influenced by different microbiota. Therefore, the in vitro model MimiCol<sup>3</sup> was used, which represents the physiological conditions of the ascending colon. In addition to a representative physiological volume, the pH value, redox potential and an anaerobic atmosphere are important to provide the bacteria with the best possible growth conditions. Stool samples were taken from three healthy subjects, comparing omnivorous, vegetarian and meat-rich diets, and cultured for 24 h. However, the nutrient medium used for cultivation led to the alignment of the bacterial composition of the microbiota. The previously observed differences between the diets could not be maintained. Nevertheless, the similar degradation of sulfasalazine was observed in all microbiota studied in MimiCol<sup>3</sup>. This makes MimiCol<sup>3</sup> a suitable in vitro model for metabolism studies in the gut microbiome. |
| first_indexed | 2024-03-10T23:39:22Z |
| format | Article |
| id | doaj.art-3c8e6386dd9647209ec07db6e4e8123c |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T23:39:22Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-3c8e6386dd9647209ec07db6e4e8123c2023-11-19T02:34:22ZengMDPI AGPharmaceuticals1424-82472023-08-01168112810.3390/ph16081128Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol<sup>3</sup>Dariah-Sohreh Seradj0Regine Beeck1Annika Haase2Julius Krause3Philipp Schick4Werner Weitschies5Center of Drug Absorption and Transport, Department of Biopharmaceutics and Pharmaceutical Technology, University of Greifswald, Felix-Hausdorff-Str. 3, D-17489 Greifswald, GermanyCenter of Drug Absorption and Transport, Department of Biopharmaceutics and Pharmaceutical Technology, University of Greifswald, Felix-Hausdorff-Str. 3, D-17489 Greifswald, GermanyCenter of Drug Absorption and Transport, Department of Biopharmaceutics and Pharmaceutical Technology, University of Greifswald, Felix-Hausdorff-Str. 3, D-17489 Greifswald, GermanyCenter of Drug Absorption and Transport, Department of Biopharmaceutics and Pharmaceutical Technology, University of Greifswald, Felix-Hausdorff-Str. 3, D-17489 Greifswald, GermanyCenter of Drug Absorption and Transport, Department of Biopharmaceutics and Pharmaceutical Technology, University of Greifswald, Felix-Hausdorff-Str. 3, D-17489 Greifswald, GermanyCenter of Drug Absorption and Transport, Department of Biopharmaceutics and Pharmaceutical Technology, University of Greifswald, Felix-Hausdorff-Str. 3, D-17489 Greifswald, GermanyThe microbiome of the colon is characterized by its great diversity. This varies not only intra- but also interindividually and is influenced by endogenous and exogenous factors, such as dietary and lifestyle factors. The aim of this work was to investigate the extent to which the degradation of the drug sulfasalazine is influenced by different microbiota. Therefore, the in vitro model MimiCol<sup>3</sup> was used, which represents the physiological conditions of the ascending colon. In addition to a representative physiological volume, the pH value, redox potential and an anaerobic atmosphere are important to provide the bacteria with the best possible growth conditions. Stool samples were taken from three healthy subjects, comparing omnivorous, vegetarian and meat-rich diets, and cultured for 24 h. However, the nutrient medium used for cultivation led to the alignment of the bacterial composition of the microbiota. The previously observed differences between the diets could not be maintained. Nevertheless, the similar degradation of sulfasalazine was observed in all microbiota studied in MimiCol<sup>3</sup>. This makes MimiCol<sup>3</sup> a suitable in vitro model for metabolism studies in the gut microbiome.https://www.mdpi.com/1424-8247/16/8/1128MimiCol<sup>3</sup>dynamic colon modelin vitro metabolizationcolonic microbiotadietsulfasalazine |
| spellingShingle | Dariah-Sohreh Seradj Regine Beeck Annika Haase Julius Krause Philipp Schick Werner Weitschies Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol<sup>3</sup> Pharmaceuticals MimiCol<sup>3</sup> dynamic colon model in vitro metabolization colonic microbiota diet sulfasalazine |
| title | Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol<sup>3</sup> |
| title_full | Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol<sup>3</sup> |
| title_fullStr | Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol<sup>3</sup> |
| title_full_unstemmed | Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol<sup>3</sup> |
| title_short | Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiCol<sup>3</sup> |
| title_sort | influence of different diets on the degradation of sulfasalazine by colon bacteria determined using mimicol sup 3 sup |
| topic | MimiCol<sup>3</sup> dynamic colon model in vitro metabolization colonic microbiota diet sulfasalazine |
| url | https://www.mdpi.com/1424-8247/16/8/1128 |
| work_keys_str_mv | AT dariahsohrehseradj influenceofdifferentdietsonthedegradationofsulfasalazinebycolonbacteriadeterminedusingmimicolsup3sup AT reginebeeck influenceofdifferentdietsonthedegradationofsulfasalazinebycolonbacteriadeterminedusingmimicolsup3sup AT annikahaase influenceofdifferentdietsonthedegradationofsulfasalazinebycolonbacteriadeterminedusingmimicolsup3sup AT juliuskrause influenceofdifferentdietsonthedegradationofsulfasalazinebycolonbacteriadeterminedusingmimicolsup3sup AT philippschick influenceofdifferentdietsonthedegradationofsulfasalazinebycolonbacteriadeterminedusingmimicolsup3sup AT wernerweitschies influenceofdifferentdietsonthedegradationofsulfasalazinebycolonbacteriadeterminedusingmimicolsup3sup |