Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches during Cold Storage
Both nitric oxide (NO) and hydrogen sulfide (H<sub>2</sub>S) have been shown to have positive effects on the maintenance of fruit quality during storage; however, the mechanisms by which NO regulates the endogenous H<sub>2</sub>S metabolism remain unknown. In this experiment,...
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| Format: | Article |
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MDPI AG
2019-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/9/401 |
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| author | Biao Geng Dandan Huang Shuhua Zhu |
| author_facet | Biao Geng Dandan Huang Shuhua Zhu |
| author_sort | Biao Geng |
| collection | DOAJ |
| description | Both nitric oxide (NO) and hydrogen sulfide (H<sub>2</sub>S) have been shown to have positive effects on the maintenance of fruit quality during storage; however, the mechanisms by which NO regulates the endogenous H<sub>2</sub>S metabolism remain unknown. In this experiment, peaches were immersed in solutions of NO, potassium 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, as an NO scavenger), N-nitro-<span style="font-variant: small-caps;">l</span>-arginine methyl ester (<span style="font-variant: small-caps;">l</span>-NAME, as an inhibitor of nitric oxide synthase (NOS)-like activity), and sodium tungstate (as an inhibitor of nitrate reductase), and the resulting changes in the H<sub>2</sub>S metabolism of peaches were studied. The results showed that exogenous NO reduced the contents of endogenous H<sub>2</sub>S, Cys, and sulfite; decreased the activities of <span style="font-variant: small-caps;">l</span>-/<span style="font-variant: small-caps;">d</span>-cysteine desulfhydrase (<span style="font-variant: small-caps;">l</span>-/<span style="font-variant: small-caps;">d</span>-CD), <i>O</i>-acetylserine (thiol)lyase (OAS-TL), and sulfite reductase (SiR); and increased the activity of β-cyanoalanine synthase (β-CAS). Both c-PTIO and sodium tungstate had similar roles in increasing the H<sub>2</sub>S content by sustaining the activities of <span style="font-variant: small-caps;">l</span>-/<span style="font-variant: small-caps;">d</span>-CDs, OAS-TL, and SiR. <span style="font-variant: small-caps;">l</span>-NAME increased the H<sub>2</sub>S content, mainly by maintaining the <span style="font-variant: small-caps;">d</span>-CD activity. The results suggest that NO, c-PTIO, <span style="font-variant: small-caps;">l</span>-NAME, and sodium tungstate differently regulate the H<sub>2</sub>S metabolism of peaches during storage. |
| first_indexed | 2024-03-12T09:43:57Z |
| format | Article |
| id | doaj.art-3c8ee39cf58742d8a9f2af98464c5452 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T09:43:57Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-3c8ee39cf58742d8a9f2af98464c54522023-09-02T13:03:21ZengMDPI AGAntioxidants2076-39212019-09-018940110.3390/antiox8090401antiox8090401Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches during Cold StorageBiao Geng0Dandan Huang1Shuhua Zhu2College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, ChinaCollege of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, ChinaCollege of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, ChinaBoth nitric oxide (NO) and hydrogen sulfide (H<sub>2</sub>S) have been shown to have positive effects on the maintenance of fruit quality during storage; however, the mechanisms by which NO regulates the endogenous H<sub>2</sub>S metabolism remain unknown. In this experiment, peaches were immersed in solutions of NO, potassium 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, as an NO scavenger), N-nitro-<span style="font-variant: small-caps;">l</span>-arginine methyl ester (<span style="font-variant: small-caps;">l</span>-NAME, as an inhibitor of nitric oxide synthase (NOS)-like activity), and sodium tungstate (as an inhibitor of nitrate reductase), and the resulting changes in the H<sub>2</sub>S metabolism of peaches were studied. The results showed that exogenous NO reduced the contents of endogenous H<sub>2</sub>S, Cys, and sulfite; decreased the activities of <span style="font-variant: small-caps;">l</span>-/<span style="font-variant: small-caps;">d</span>-cysteine desulfhydrase (<span style="font-variant: small-caps;">l</span>-/<span style="font-variant: small-caps;">d</span>-CD), <i>O</i>-acetylserine (thiol)lyase (OAS-TL), and sulfite reductase (SiR); and increased the activity of β-cyanoalanine synthase (β-CAS). Both c-PTIO and sodium tungstate had similar roles in increasing the H<sub>2</sub>S content by sustaining the activities of <span style="font-variant: small-caps;">l</span>-/<span style="font-variant: small-caps;">d</span>-CDs, OAS-TL, and SiR. <span style="font-variant: small-caps;">l</span>-NAME increased the H<sub>2</sub>S content, mainly by maintaining the <span style="font-variant: small-caps;">d</span>-CD activity. The results suggest that NO, c-PTIO, <span style="font-variant: small-caps;">l</span>-NAME, and sodium tungstate differently regulate the H<sub>2</sub>S metabolism of peaches during storage.https://www.mdpi.com/2076-3921/8/9/401nitric oxidehydrogen sulfidepeachstoragemetabolismquality |
| spellingShingle | Biao Geng Dandan Huang Shuhua Zhu Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches during Cold Storage Antioxidants nitric oxide hydrogen sulfide peach storage metabolism quality |
| title | Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches during Cold Storage |
| title_full | Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches during Cold Storage |
| title_fullStr | Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches during Cold Storage |
| title_full_unstemmed | Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches during Cold Storage |
| title_short | Regulation of Hydrogen Sulfide Metabolism by Nitric Oxide Inhibitors and the Quality of Peaches during Cold Storage |
| title_sort | regulation of hydrogen sulfide metabolism by nitric oxide inhibitors and the quality of peaches during cold storage |
| topic | nitric oxide hydrogen sulfide peach storage metabolism quality |
| url | https://www.mdpi.com/2076-3921/8/9/401 |
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