Evaluation of in-vitro antibacterial effect of crude venom of Pseudocerastes Persicus snake
Objectives: This study aimed to assess the antibacterial activity of the crude venom of Pseudocerastes persicus against some Gram-negative and Gram-positive bacteria using an antimicrobial susceptibility test.Methods: The susceptibility of Methicillin-Resistant Staphylococcus aureus (MRSA), Listeria...
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| Format: | Article |
| Language: | English |
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Kashan University of Medical Sciences
2024-02-01
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| Series: | International Archives of Health Sciences |
| Subjects: | |
| Online Access: | http://iahsj.kaums.ac.ir/article_187499_f2cad41c32b0bc8f6cce72c59f4dfe85.pdf |
| _version_ | 1797220325450055680 |
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| author | Behrooz Fathi Abdoulah Jamshidi Sadaf Sabzevari |
| author_facet | Behrooz Fathi Abdoulah Jamshidi Sadaf Sabzevari |
| author_sort | Behrooz Fathi |
| collection | DOAJ |
| description | Objectives: This study aimed to assess the antibacterial activity of the crude venom of Pseudocerastes persicus against some Gram-negative and Gram-positive bacteria using an antimicrobial susceptibility test.Methods: The susceptibility of Methicillin-Resistant Staphylococcus aureus (MRSA), Listeria monocytogenes, Bacillus subtilis, Salmonella typhimurium, and E. coli O157:H7 to the crude venom of Pseudocerastes persicus was investigated at a concentration of 100 µg/ml. Standard antibiotic disks were utilized as positive controls. Furthermore, the minimum inhibitory concentration (MIC) against Staphylococcus aureus and MRSA was determined through the dilution method (160-1.25 µg/ml). These MIC values were compared with those of conventional drugs such as streptomycin (25 µg), tetracycline (30 µg), and neomycin (25 µg).Results: The crude venom exhibited significant antibacterial activity against Staphylococcus aureus, MRSA, Listeria monocytogenes, and E. coli O157:H7. It displayed moderate effects on Salmonella typhimurium but showed no significant impact on Bacillus subtilis. The MIC values against these bacteria ranged from 160 to 80 µg/ml.Conclusion: The venom from Pseudocerastes persicus demonstrates antibacterial properties and shows potential therapeutic value. Further investigations involving fractionation are necessary to fully explore its therapeutic potential. |
| first_indexed | 2024-04-24T12:47:45Z |
| format | Article |
| id | doaj.art-3c8fca94e5914ba1b8d937b25a340366 |
| institution | Directory Open Access Journal |
| issn | 2383-2568 |
| language | English |
| last_indexed | 2024-04-24T12:47:45Z |
| publishDate | 2024-02-01 |
| publisher | Kashan University of Medical Sciences |
| record_format | Article |
| series | International Archives of Health Sciences |
| spelling | doaj.art-3c8fca94e5914ba1b8d937b25a3403662024-04-06T16:39:10ZengKashan University of Medical SciencesInternational Archives of Health Sciences2383-25682024-02-01111364110.48307/iahsj.2023.413997.1053187499Evaluation of in-vitro antibacterial effect of crude venom of Pseudocerastes Persicus snakeBehrooz Fathi0Abdoulah Jamshidi1Sadaf Sabzevari2Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranObjectives: This study aimed to assess the antibacterial activity of the crude venom of Pseudocerastes persicus against some Gram-negative and Gram-positive bacteria using an antimicrobial susceptibility test.Methods: The susceptibility of Methicillin-Resistant Staphylococcus aureus (MRSA), Listeria monocytogenes, Bacillus subtilis, Salmonella typhimurium, and E. coli O157:H7 to the crude venom of Pseudocerastes persicus was investigated at a concentration of 100 µg/ml. Standard antibiotic disks were utilized as positive controls. Furthermore, the minimum inhibitory concentration (MIC) against Staphylococcus aureus and MRSA was determined through the dilution method (160-1.25 µg/ml). These MIC values were compared with those of conventional drugs such as streptomycin (25 µg), tetracycline (30 µg), and neomycin (25 µg).Results: The crude venom exhibited significant antibacterial activity against Staphylococcus aureus, MRSA, Listeria monocytogenes, and E. coli O157:H7. It displayed moderate effects on Salmonella typhimurium but showed no significant impact on Bacillus subtilis. The MIC values against these bacteria ranged from 160 to 80 µg/ml.Conclusion: The venom from Pseudocerastes persicus demonstrates antibacterial properties and shows potential therapeutic value. Further investigations involving fractionation are necessary to fully explore its therapeutic potential.http://iahsj.kaums.ac.ir/article_187499_f2cad41c32b0bc8f6cce72c59f4dfe85.pdfpseudocerastes persicussnakeantibacterial effect |
| spellingShingle | Behrooz Fathi Abdoulah Jamshidi Sadaf Sabzevari Evaluation of in-vitro antibacterial effect of crude venom of Pseudocerastes Persicus snake International Archives of Health Sciences pseudocerastes persicus snake antibacterial effect |
| title | Evaluation of in-vitro antibacterial effect of crude venom of Pseudocerastes Persicus snake |
| title_full | Evaluation of in-vitro antibacterial effect of crude venom of Pseudocerastes Persicus snake |
| title_fullStr | Evaluation of in-vitro antibacterial effect of crude venom of Pseudocerastes Persicus snake |
| title_full_unstemmed | Evaluation of in-vitro antibacterial effect of crude venom of Pseudocerastes Persicus snake |
| title_short | Evaluation of in-vitro antibacterial effect of crude venom of Pseudocerastes Persicus snake |
| title_sort | evaluation of in vitro antibacterial effect of crude venom of pseudocerastes persicus snake |
| topic | pseudocerastes persicus snake antibacterial effect |
| url | http://iahsj.kaums.ac.ir/article_187499_f2cad41c32b0bc8f6cce72c59f4dfe85.pdf |
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