An analysis on the divergence of Chironomid spp. based on the study of 18S rRNA and polytene chromosome organization in the species revealing the role of environment on speciation
Abstract Background Nine species of Chironomus evolved throughout the world were measured for their divergence with regard to their DNA sequences concerning 18S rRNA since it is conserved for a specific species. With the advancement of the field of molecular evolution, cytogenetics requires further...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-04-01
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| Series: | Journal of Basic and Applied Zoology |
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| Online Access: | https://doi.org/10.1186/s41936-021-00215-0 |
| _version_ | 1818580019843170304 |
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| author | Sanjay Kumar Dey Swapna Bhaduri Trilochan Midya |
| author_facet | Sanjay Kumar Dey Swapna Bhaduri Trilochan Midya |
| author_sort | Sanjay Kumar Dey |
| collection | DOAJ |
| description | Abstract Background Nine species of Chironomus evolved throughout the world were measured for their divergence with regard to their DNA sequences concerning 18S rRNA since it is conserved for a specific species. With the advancement of the field of molecular evolution, cytogenetics requires further correlation between molecular architecture and morphological features of a species to compare amongst others to decipher their role in speciation. Therefore, divergence of DNA sequences of the Chironomus were compared with differences in the polytene chromosome features of most of the species under this investigation to evaluate underlying correlation among them, if any, to finally establish a novel method of molecular classification broadly applicable in cytogenetics studies. Results When Chironomus javanus Kieffer was considered as a reference organism, an in silico pair-wise alignment of sequences for the 18S rRNA gene regions of the other eight different species of the same genus exhibited nucleotide sequence homology ranging from 67 to 98%. This divergence of the species under consideration might be due to environmental impact causing alteration of nitrogenous bases probably due to mismatch pairing in DNA replication. This may be suggested as a cause of evolution of species in nature. A concomitant study on the polytene chromosome band patterns of majority of these species belonging to this series also indicated a divergence ranging from 10% to 30%. Conclusions Sequence analysis based on 18S rRNA of nine species of Chironomus under this investigation shows a similarity in the polytene chromosome organization in most of the Chironomid species of the series. Hence, molecular divergence in the species is consistent with cytological difference among Chironomid species. Therefore, molecular data based on 18S rRNA and cytological characters based on the polytene chromosome features of the Chironomid species may be useful for their taxonomical recognition. Moreover, variations concerning two aspects of this study may be correlated to their environmental distinctions. |
| first_indexed | 2024-12-16T07:10:57Z |
| format | Article |
| id | doaj.art-3c8feaf5902a47b99d347ec61e9f736f |
| institution | Directory Open Access Journal |
| issn | 2090-990X |
| language | English |
| last_indexed | 2024-12-16T07:10:57Z |
| publishDate | 2021-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Basic and Applied Zoology |
| spelling | doaj.art-3c8feaf5902a47b99d347ec61e9f736f2022-12-21T22:39:54ZengSpringerOpenJournal of Basic and Applied Zoology2090-990X2021-04-0182111210.1186/s41936-021-00215-0An analysis on the divergence of Chironomid spp. based on the study of 18S rRNA and polytene chromosome organization in the species revealing the role of environment on speciationSanjay Kumar Dey0Swapna Bhaduri1Trilochan Midya2Department of Zoology and Molecular Biology & Genetics, Presidency UniversityDepartment of Zoology and Molecular Biology & Genetics, Presidency UniversityDepartment of Zoology and Molecular Biology & Genetics, Presidency UniversityAbstract Background Nine species of Chironomus evolved throughout the world were measured for their divergence with regard to their DNA sequences concerning 18S rRNA since it is conserved for a specific species. With the advancement of the field of molecular evolution, cytogenetics requires further correlation between molecular architecture and morphological features of a species to compare amongst others to decipher their role in speciation. Therefore, divergence of DNA sequences of the Chironomus were compared with differences in the polytene chromosome features of most of the species under this investigation to evaluate underlying correlation among them, if any, to finally establish a novel method of molecular classification broadly applicable in cytogenetics studies. Results When Chironomus javanus Kieffer was considered as a reference organism, an in silico pair-wise alignment of sequences for the 18S rRNA gene regions of the other eight different species of the same genus exhibited nucleotide sequence homology ranging from 67 to 98%. This divergence of the species under consideration might be due to environmental impact causing alteration of nitrogenous bases probably due to mismatch pairing in DNA replication. This may be suggested as a cause of evolution of species in nature. A concomitant study on the polytene chromosome band patterns of majority of these species belonging to this series also indicated a divergence ranging from 10% to 30%. Conclusions Sequence analysis based on 18S rRNA of nine species of Chironomus under this investigation shows a similarity in the polytene chromosome organization in most of the Chironomid species of the series. Hence, molecular divergence in the species is consistent with cytological difference among Chironomid species. Therefore, molecular data based on 18S rRNA and cytological characters based on the polytene chromosome features of the Chironomid species may be useful for their taxonomical recognition. Moreover, variations concerning two aspects of this study may be correlated to their environmental distinctions.https://doi.org/10.1186/s41936-021-00215-0ChironomusPolytene chromosome18S rRNAPair-wise alignmentConserved sitesBiological indicator |
| spellingShingle | Sanjay Kumar Dey Swapna Bhaduri Trilochan Midya An analysis on the divergence of Chironomid spp. based on the study of 18S rRNA and polytene chromosome organization in the species revealing the role of environment on speciation Journal of Basic and Applied Zoology Chironomus Polytene chromosome 18S rRNA Pair-wise alignment Conserved sites Biological indicator |
| title | An analysis on the divergence of Chironomid spp. based on the study of 18S rRNA and polytene chromosome organization in the species revealing the role of environment on speciation |
| title_full | An analysis on the divergence of Chironomid spp. based on the study of 18S rRNA and polytene chromosome organization in the species revealing the role of environment on speciation |
| title_fullStr | An analysis on the divergence of Chironomid spp. based on the study of 18S rRNA and polytene chromosome organization in the species revealing the role of environment on speciation |
| title_full_unstemmed | An analysis on the divergence of Chironomid spp. based on the study of 18S rRNA and polytene chromosome organization in the species revealing the role of environment on speciation |
| title_short | An analysis on the divergence of Chironomid spp. based on the study of 18S rRNA and polytene chromosome organization in the species revealing the role of environment on speciation |
| title_sort | analysis on the divergence of chironomid spp based on the study of 18s rrna and polytene chromosome organization in the species revealing the role of environment on speciation |
| topic | Chironomus Polytene chromosome 18S rRNA Pair-wise alignment Conserved sites Biological indicator |
| url | https://doi.org/10.1186/s41936-021-00215-0 |
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