Antimicrobial Susceptibility of Salmonella Isolated from Chickens and Humans in Wau, South Sudan
Background. Salmonella infections are a public health problem across the globe. In South Sudan, there is little information regarding the prevalence and antibiotic resistance patterns of Salmonella. Therefore, this study assessed the prevalence and antimicrobial susceptibility of Salmonella isolates...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2022-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/8570081 |
| _version_ | 1797797253252907008 |
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| author | Shereen Saad Ambros Jubara Charles Wani Musso Munyeme Alfateh Taha Steward Mudenda Mildred Zulu Wizaso Mwasinga Mulemba Samutela Rabecca Tembo Walter Muleya Geoffrey Kwenda Bernard Mudenda Hang’ombe |
| author_facet | Shereen Saad Ambros Jubara Charles Wani Musso Munyeme Alfateh Taha Steward Mudenda Mildred Zulu Wizaso Mwasinga Mulemba Samutela Rabecca Tembo Walter Muleya Geoffrey Kwenda Bernard Mudenda Hang’ombe |
| author_sort | Shereen Saad |
| collection | DOAJ |
| description | Background. Salmonella infections are a public health problem across the globe. In South Sudan, there is little information regarding the prevalence and antibiotic resistance patterns of Salmonella. Therefore, this study assessed the prevalence and antimicrobial susceptibility of Salmonella isolates from chickens and humans in South Sudan. Fecal samples were collected and cultured on Xylose Lysine Deoxycholate Agar for the isolation of Salmonella and confirmed using biochemical tests and PCR through the amplification of the invA gene. A total of 417 fecal samples were examined, of which 270 (64.7%) were chicken cloacal swabs while 147 (35.3%) were humans’ stool specimens. Results. Eleven (11) Salmonella isolates were isolated from humans while nine were from chickens. All 11 isolates from humans were susceptible to sulfamethoxazole-trimethoprim, chloramphenicol, streptomycin, cefotaxime, nalidixic acid, and gentamicin. However, 4 (36.7%) isolates showed resistance to ciprofloxacin, 2 (18.9%) to ampicillin, and 1 (9.1%) to tetracycline. All chicken isolates were susceptible to chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim, ciprofloxacin, cefotaxime, nalidixic acid, and gentamicin but showed resistance to tetracycline 2 (22.2%) and ampicillin 1 (11.1%). Conclusion. Antimicrobial resistant isolates were isolated in both chickens and humans. Further, MDR isolates were found in both chicken and human samples, and this is a public health concern. This, therefore, calls for concerted efforts to educate producers and consumers on public health, food safety, food hygiene in food production, and enhancement of surveillance programmes on zoonotic bacteria and antimicrobial susceptibility. |
| first_indexed | 2024-03-13T03:45:22Z |
| format | Article |
| id | doaj.art-3c916995b856441d88e4367143b805a5 |
| institution | Directory Open Access Journal |
| issn | 1687-9198 |
| language | English |
| last_indexed | 2024-03-13T03:45:22Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj.art-3c916995b856441d88e4367143b805a52023-06-23T00:00:07ZengHindawi LimitedInternational Journal of Microbiology1687-91982022-01-01202210.1155/2022/8570081Antimicrobial Susceptibility of Salmonella Isolated from Chickens and Humans in Wau, South SudanShereen Saad0Ambros Jubara1Charles Wani2Musso Munyeme3Alfateh Taha4Steward Mudenda5Mildred Zulu6Wizaso Mwasinga7Mulemba Samutela8Rabecca Tembo9Walter Muleya10Geoffrey Kwenda11Bernard Mudenda Hang’ombe12Department of Clinical StudiesDepartment of Clinical StudiesDepartment of Clinical StudiesDepartment of Disease ControlDepartment of Clinical StudiesDepartment of PharmacyDepartment of Pathology and MicrobiologyDepartment of Disease ControlDepartment of Biomedical SciencesDepartment of Pathology and MicrobiologyDepartment of BiomedicalDepartment of PharmacyDepartment of Paraclinical StudiesBackground. Salmonella infections are a public health problem across the globe. In South Sudan, there is little information regarding the prevalence and antibiotic resistance patterns of Salmonella. Therefore, this study assessed the prevalence and antimicrobial susceptibility of Salmonella isolates from chickens and humans in South Sudan. Fecal samples were collected and cultured on Xylose Lysine Deoxycholate Agar for the isolation of Salmonella and confirmed using biochemical tests and PCR through the amplification of the invA gene. A total of 417 fecal samples were examined, of which 270 (64.7%) were chicken cloacal swabs while 147 (35.3%) were humans’ stool specimens. Results. Eleven (11) Salmonella isolates were isolated from humans while nine were from chickens. All 11 isolates from humans were susceptible to sulfamethoxazole-trimethoprim, chloramphenicol, streptomycin, cefotaxime, nalidixic acid, and gentamicin. However, 4 (36.7%) isolates showed resistance to ciprofloxacin, 2 (18.9%) to ampicillin, and 1 (9.1%) to tetracycline. All chicken isolates were susceptible to chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim, ciprofloxacin, cefotaxime, nalidixic acid, and gentamicin but showed resistance to tetracycline 2 (22.2%) and ampicillin 1 (11.1%). Conclusion. Antimicrobial resistant isolates were isolated in both chickens and humans. Further, MDR isolates were found in both chicken and human samples, and this is a public health concern. This, therefore, calls for concerted efforts to educate producers and consumers on public health, food safety, food hygiene in food production, and enhancement of surveillance programmes on zoonotic bacteria and antimicrobial susceptibility.http://dx.doi.org/10.1155/2022/8570081 |
| spellingShingle | Shereen Saad Ambros Jubara Charles Wani Musso Munyeme Alfateh Taha Steward Mudenda Mildred Zulu Wizaso Mwasinga Mulemba Samutela Rabecca Tembo Walter Muleya Geoffrey Kwenda Bernard Mudenda Hang’ombe Antimicrobial Susceptibility of Salmonella Isolated from Chickens and Humans in Wau, South Sudan International Journal of Microbiology |
| title | Antimicrobial Susceptibility of Salmonella Isolated from Chickens and Humans in Wau, South Sudan |
| title_full | Antimicrobial Susceptibility of Salmonella Isolated from Chickens and Humans in Wau, South Sudan |
| title_fullStr | Antimicrobial Susceptibility of Salmonella Isolated from Chickens and Humans in Wau, South Sudan |
| title_full_unstemmed | Antimicrobial Susceptibility of Salmonella Isolated from Chickens and Humans in Wau, South Sudan |
| title_short | Antimicrobial Susceptibility of Salmonella Isolated from Chickens and Humans in Wau, South Sudan |
| title_sort | antimicrobial susceptibility of salmonella isolated from chickens and humans in wau south sudan |
| url | http://dx.doi.org/10.1155/2022/8570081 |
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