Antitubercular activity of the pigment from forest soil Streptomyces sp SFA5
Extracellular pigment from the forest soil Streptomyces sp SFA5 was produced by submerged fermentation using yeast extract malt extract broth. Crude pigment from the medium was extracted using ethyl acetate. Antitubercular activity of the pigment was tested against M. tuberculosis H37Rv by microplat...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2015-12-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/24238 |
| _version_ | 1831730625633058816 |
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| author | Radhakrishnan Manikkam Shekar Ponnuswamy Jerrine Joseph Vanaja Kumar |
| author_facet | Radhakrishnan Manikkam Shekar Ponnuswamy Jerrine Joseph Vanaja Kumar |
| author_sort | Radhakrishnan Manikkam |
| collection | DOAJ |
| description | Extracellular pigment from the forest soil Streptomyces sp SFA5 was produced by submerged fermentation using yeast extract malt extract broth. Crude pigment from the medium was extracted using ethyl acetate. Antitubercular activity of the pigment was tested against M. tuberculosis H37Rv by microplate alamar blue assay and luciferase reporter phage assay. The pigment was also tested for inhibitory activity against M. tuberculosis lysine aminotransferase by colorimetric method. In both microplate alamar blue and luciferase reporter phage assay, the crude pigment showed activity against M. tuberculosis H37Rv at 125 and 250 µg/mL concentration, respectively. The M. tuberculosis lysine aminotransferase was inhibited at the IC50 value of 4.5 µg/mL concentration. |
| first_indexed | 2024-12-21T09:49:52Z |
| format | Article |
| id | doaj.art-3c922324ed7142bd9646ec7a8d0646e2 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-21T09:49:52Z |
| publishDate | 2015-12-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-3c922324ed7142bd9646ec7a8d0646e22022-12-21T19:08:13ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882015-12-0111110.3329/bjp.v11i1.24238Antitubercular activity of the pigment from forest soil Streptomyces sp SFA5Radhakrishnan Manikkam0Shekar Ponnuswamy1Jerrine Joseph2Vanaja Kumar3Centre for Drug Discovery and Development, Sathyabama University, Chennai 600119Department of Chemical Engineering, SSN College of Engineering, Chennai 603110Centre for Drug Discovery and Development, Sathyabama University, Chennai 600119Centre for Drug Discovery and Development, Sathyabama University, Chennai 600119Extracellular pigment from the forest soil Streptomyces sp SFA5 was produced by submerged fermentation using yeast extract malt extract broth. Crude pigment from the medium was extracted using ethyl acetate. Antitubercular activity of the pigment was tested against M. tuberculosis H37Rv by microplate alamar blue assay and luciferase reporter phage assay. The pigment was also tested for inhibitory activity against M. tuberculosis lysine aminotransferase by colorimetric method. In both microplate alamar blue and luciferase reporter phage assay, the crude pigment showed activity against M. tuberculosis H37Rv at 125 and 250 µg/mL concentration, respectively. The M. tuberculosis lysine aminotransferase was inhibited at the IC50 value of 4.5 µg/mL concentration.https://www.banglajol.info/index.php/BJP/article/view/24238AntitubercularForest soilStreptomyces |
| spellingShingle | Radhakrishnan Manikkam Shekar Ponnuswamy Jerrine Joseph Vanaja Kumar Antitubercular activity of the pigment from forest soil Streptomyces sp SFA5 Bangladesh Journal of Pharmacology Antitubercular Forest soil Streptomyces |
| title | Antitubercular activity of the pigment from forest soil Streptomyces sp SFA5 |
| title_full | Antitubercular activity of the pigment from forest soil Streptomyces sp SFA5 |
| title_fullStr | Antitubercular activity of the pigment from forest soil Streptomyces sp SFA5 |
| title_full_unstemmed | Antitubercular activity of the pigment from forest soil Streptomyces sp SFA5 |
| title_short | Antitubercular activity of the pigment from forest soil Streptomyces sp SFA5 |
| title_sort | antitubercular activity of the pigment from forest soil streptomyces sp sfa5 |
| topic | Antitubercular Forest soil Streptomyces |
| url | https://www.banglajol.info/index.php/BJP/article/view/24238 |
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