Alpha-pinene ameliorate behavioral deficit induced by early postnatal hypoxia in the rat: study the inflammatory mechanism
Abstract Neonatal hypoxia has a negative impact on the developing brain during the sensitive period. Inflammation plays a key role in the physiological response to hypoxic stress. Considering the anti-inflammatory properties of alpha-pinene, which has received a lot of attention in recent years, in...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-56756-1 |
| _version_ | 1827316044081922048 |
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| author | Sharareh Bakhtazad Zohreh Ghotbeddin Mohammad Reza Tabandeh Kaveh Rahimi |
| author_facet | Sharareh Bakhtazad Zohreh Ghotbeddin Mohammad Reza Tabandeh Kaveh Rahimi |
| author_sort | Sharareh Bakhtazad |
| collection | DOAJ |
| description | Abstract Neonatal hypoxia has a negative impact on the developing brain during the sensitive period. Inflammation plays a key role in the physiological response to hypoxic stress. Considering the anti-inflammatory properties of alpha-pinene, which has received a lot of attention in recent years, in this research we focused on the impact of alpha-pinene on the behavioral responses and proinflammatory factors in rats subjected to the neonatal hypoxia. This study involved Wistar rats (7-day-old) that were divided into six experimental groups, including a control group, groups receiving different doses of alpha-pinene (5 and 10 mg/kg), a hypoxia group receiving 7% O2 and 93% N2, 90 min duration for 7 days, and groups receiving alpha-pinene 30 min before hypoxia. All injections were done intraperitoneally. The rats were evaluated for proinflammatory factors 24 h after exposure to hypoxia (PND14) and at the end of the behavioral test (PND54). The results showed that hypoxia led to decreased motor activity, coordination, and memory, as well as increased inflammation. However, the rats that received alpha-pinene showed improved behavioral responses and reduced inflammation compared to the hypoxia group (all cases p < 0.05). This suggests that alpha-pinene may have a protective effect via anti-inflammatory properties against the negative impacts of hypoxia on the developing brain. |
| first_indexed | 2024-04-24T23:06:49Z |
| format | Article |
| id | doaj.art-3c930e25c5754146add4ffdd0aa30db7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T23:06:49Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3c930e25c5754146add4ffdd0aa30db72024-03-17T12:25:35ZengNature PortfolioScientific Reports2045-23222024-03-0114111210.1038/s41598-024-56756-1Alpha-pinene ameliorate behavioral deficit induced by early postnatal hypoxia in the rat: study the inflammatory mechanismSharareh Bakhtazad0Zohreh Ghotbeddin1Mohammad Reza Tabandeh2Kaveh Rahimi3Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of AhvazDepartment of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of AhvazStem Cell and Transgenic Technology Research Center, Shahid Chamran University of AhvazDepartment of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of AhvazAbstract Neonatal hypoxia has a negative impact on the developing brain during the sensitive period. Inflammation plays a key role in the physiological response to hypoxic stress. Considering the anti-inflammatory properties of alpha-pinene, which has received a lot of attention in recent years, in this research we focused on the impact of alpha-pinene on the behavioral responses and proinflammatory factors in rats subjected to the neonatal hypoxia. This study involved Wistar rats (7-day-old) that were divided into six experimental groups, including a control group, groups receiving different doses of alpha-pinene (5 and 10 mg/kg), a hypoxia group receiving 7% O2 and 93% N2, 90 min duration for 7 days, and groups receiving alpha-pinene 30 min before hypoxia. All injections were done intraperitoneally. The rats were evaluated for proinflammatory factors 24 h after exposure to hypoxia (PND14) and at the end of the behavioral test (PND54). The results showed that hypoxia led to decreased motor activity, coordination, and memory, as well as increased inflammation. However, the rats that received alpha-pinene showed improved behavioral responses and reduced inflammation compared to the hypoxia group (all cases p < 0.05). This suggests that alpha-pinene may have a protective effect via anti-inflammatory properties against the negative impacts of hypoxia on the developing brain.https://doi.org/10.1038/s41598-024-56756-1Alpha-pineneBehavioral responsesHypoxiaInflammationRat |
| spellingShingle | Sharareh Bakhtazad Zohreh Ghotbeddin Mohammad Reza Tabandeh Kaveh Rahimi Alpha-pinene ameliorate behavioral deficit induced by early postnatal hypoxia in the rat: study the inflammatory mechanism Scientific Reports Alpha-pinene Behavioral responses Hypoxia Inflammation Rat |
| title | Alpha-pinene ameliorate behavioral deficit induced by early postnatal hypoxia in the rat: study the inflammatory mechanism |
| title_full | Alpha-pinene ameliorate behavioral deficit induced by early postnatal hypoxia in the rat: study the inflammatory mechanism |
| title_fullStr | Alpha-pinene ameliorate behavioral deficit induced by early postnatal hypoxia in the rat: study the inflammatory mechanism |
| title_full_unstemmed | Alpha-pinene ameliorate behavioral deficit induced by early postnatal hypoxia in the rat: study the inflammatory mechanism |
| title_short | Alpha-pinene ameliorate behavioral deficit induced by early postnatal hypoxia in the rat: study the inflammatory mechanism |
| title_sort | alpha pinene ameliorate behavioral deficit induced by early postnatal hypoxia in the rat study the inflammatory mechanism |
| topic | Alpha-pinene Behavioral responses Hypoxia Inflammation Rat |
| url | https://doi.org/10.1038/s41598-024-56756-1 |
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